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Plasmid#22661DepositorInsertLamin A (LMNA Human)
UseLentiviralTagsOnestrep TagExpressionMammalianMutationPromoterAvailable sinceFeb. 4, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCDHblast MCSNard OST-LMNAd50
Plasmid#22662DepositorInsertLaminAd50 (=Progerin)
UseLentiviralTagsOnestrep TagExpressionMammalianMutation50AA deletion creating Progerin instead of mature…PromoterAvailable sinceDec. 29, 2009AvailabilityAcademic Institutions and Nonprofits only -
pGL0_20 [panARS-opt ORI]
Plasmid#198933PurposeLevel 0 partDepositorInsertpanARS-opt ORI
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(-)-C2C1-ErNaR-TS-EYFP-ES
Plasmid#209851PurposeExpression of human codon-optimized light-driven sodium pump ErNaR in mammalian cellsDepositorInsertlight-driven sodium pump ErNaR
UseTagsEYFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pT7-V5-SBP-C1-HsUpf1-siRNAres_P
Plasmid#147196PurposeMammalian Expression of HsUpf1-siRNAresDepositorInsertHsUpf1-siRNAres (UPF1 Human)
UseTagsExpressionMammalianMutation5 non silent mutations A69S, E167G, K282R, A442G…PromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEBG-IFNAR2(265-515)
Plasmid#12437DepositorInsertIFNAR2 a.a. 265-515 (IFNAR2 Human)
UseTagsGSTExpressionMammalianMutationpEBG vector is from Bruce Mayer, may be different…PromoterAvailable sinceJan. 4, 2007AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S(-10AC12)_Psyn-sgRNArodA
Plasmid#149661Purposeall-in-one CRISPRi vector for targeting B. burgdorferi rodADepositorInsertdCas9, lacI, sgRNArodA
UseCRISPRTagsExpressionBacterialMutationPromoterPflaB, PpQE30(-10AC12), PsynAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLNCX2-mCherry-CHMP2A-siRNAres
Plasmid#115332Purposeexpresses siRNA-resistant mCherry-CHMP2A in mammalian cellsDepositorInsertCHMP2A (CHMP2A Human)
UseRetroviralTagsmCherryExpressionMammalianMutationmutated to be resistant to siRNA knockdownPromoterCMVAvailable sinceSept. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
PX390 Flavobacterium columnare Cas9
Plasmid#68327PurposeFlavobacterium columnare Cas9DepositorInsertFcCas9
UseCRISPRTagsHA and NLSExpressionMammalianMutationPromoterCMVAvailable sinceAug. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S(RBSmut)_Psyn-sgRNArodA
Plasmid#149656Purposeall-in-one CRISPRi vector for targeting B. burgdorferi rodADepositorInsertdCas9, lacI, sgRNArodA
UseCRISPRTagsExpressionBacterialMutationPromoterPflaB, PpQE30, PsynAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
Bov.hsc70.RSET.a1-554.wt(NarI)
Plasmid#12533DepositorInsertBovine 70 kDa heat shock cognate protein fragment amino acids 1-554
UseTagsExpressionBacterialMutationdeleted carboxy-terminal residues 555-650 of bovi…PromoterAvailable sinceSept. 1, 2006AvailabilityAcademic Institutions and Nonprofits only -
pMXS-IP DEPP1 dTSnare-mCherry
Plasmid#224561PurposeExpresses DEPP1 dTsnare fused to mCherry in mammalian cellsDepositorInsertDEPP1 (DEPP1 Human)
UseRetroviralTagsmCherryExpressionMammalianMutationremoved Tsnare domainPromoterAvailable sinceFeb. 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJL CusS NarX Chimera
Plasmid#225652PurposeExpresses a NarX-based chimera capable of sensing copper (II). For use with NarL-YdfI and reporter plasmids from this study.DepositorInsertCusS-NarX
UseCell free expressionTagsMycExpressionBacterialMutationTransmembrane and sensing domain replaced to dete…PromoterT7Available sinceDec. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJL NarL YdfI Flag
Plasmid#225650PurposeExpresses NarL with a mutated DNA binding domain (YdfI). For use with NarX chimeras and reporter plasmids from this study.DepositorInsertNarL-YdfI (narL Synthetic)
UseCell free expressionTagsFLAGExpressionBacterialMutationNarL with native DNA-binding domain replaced with…PromoterT7Available sinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJL NrsS NarX Chimera
Plasmid#225653PurposeExpresses a NarX-based chimera capable of sensing nickel (II). For use with NarL-YdfI and reporter plasmids from this study.DepositorInsertNrsS-NarX
UseCell free expressionTagsMycExpressionBacterialMutationTransmembrane and sensing domain replaced to dete…PromoterT7Available sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJL RssA NarX Chimera
Plasmid#225654PurposeExpresses a NarX-based chimera capable of sensing iron (III). For use with NarL-YdfI and reporter plasmids from this study.DepositorInsertRssA-NarX
UseCell free expressionTagsMycExpressionBacterialMutationTransmembrane and sensing domain replaced to dete…PromoterT7Available sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJL VanS NarX Chimera
Plasmid#225655PurposeExpresses a NarX-based chimera capable of sensing vancomycin. For use with NarL-YdfI and reporter plasmids from this study.DepositorInsertVanS-NarX
UseCell free expressionTagsMycExpressionBacterialMutationTransmembrane and sensing domain replaced to dete…PromoterT7Available sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
mCherry-C1-FKBPflex-OSBP-siRNAr
Plasmid#220066PurposesiRNA resistant ORP5 attcahed with anFKBP and a mCherry fluorophoreDepositorInsertOSBPL5 siRNA resistant (OSBPL5 Human)
UseTagsExpressionMammalianMutationsiRNA resistantPromoterAvailable sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-HA-DmGIGYF_1-640-dsRNAres_AH
Plasmid#148926PurposeInsect Expression of DmGIGYF1_1-640-dsRNAresDepositorInsertDmGIGYF1_1-640-dsRNAres (Gyf Fly)
UseTagsExpressionInsectMutationone silent mutation compared to the sequence give…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-HA-DmGIGYF-Y58AY60AM65A-dsRNAres_AH
Plasmid#148929PurposeInsect Expression of DmGIGYF1-Y58AY60AM65A-dsRNAresDepositorInsertDmGIGYF1-Y58AY60AM65A-dsRNAres (Gyf Fly)
UseTagsExpressionInsectMutationone silent mutation compared to the sequence give…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only