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Showing: 61 - 80 of 8975 results

  2. pCG-A-sacB(SP)-B

    Plasmid
    #87370
    Purpose
    An ORF part-containing vector harbouring the signal peptide from SacB with 4 bp overhangs specific for the ‘signal peptide’ position within an ORF assembly
    Depositor
    Tom Ellis
    Article
    Gilbert et al ACS Synth Biol. 2017 Mar 7. doi: 10.1021
    Insert
    SacB signal peptide
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    87370-plasmid-map-sequence-id-183403

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  3. pVP65KR-gb-SacB(-)

    Plasmid
    #171646
    Purpose
    Equipped with an autoinducible promoter. Possible sucrose selection. Works in a general host and in a general medium. Real-time monitoring of the protein production. 8xHis at the N-terminus.
    Depositor
    Young Kee Chae
    Article
    Chae et al Protein Pept Lett. 2021;28(12):1398-1407
    Insert
    MBP-mCherry-8xHis-SacB(-)
    Use
    Tags
    8xHis
    Expression
    Mutation
    internal HindIII site in the SacB gene was remove…
    Promoter
    Available since
    July 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    171646-plasmid-map-sequence-id-338426

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  4. pcDNA3-sACE2v1

    Plasmid
    #145148
    Purpose
    Mammalian expression plasmid for soluble ACE2 (protease domain) high affinity variant 1
    Depositor
    Erik Procko
    Article
    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.
    Insert
    Angiotensin-converting enzyme 2 (ACE2 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Extracellular, soluble protease domain (a.a. M1-D…
    Promoter
    CMV
    Available since
    April 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    145148-plasmid-map-sequence-id-292584

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  7. pME_Cp_0_3_018_psaC_5'UTR_Cr

    Plasmid
    #235843
    Purpose
    psaC 5'UTR Chlamydomonas reinhardtii
    Depositor
    Tobias Erb
    Insert
    psaC 5'UTR
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    none
    Promoter
    Available since
    May 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    235843-plasmid-map-sequence-id-471446

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  11. DG SaCas9 GFP V3

    Plasmid
    #226961
    Purpose
    CBh-SaCas9-2A-GFP, and 2X hU6-sgRNA (Sa) with BbsI golden gate cloning backbone for dual gRNAs. sgRNA uses 3TC scaffold for increased sgRNA expression.
    Depositor
    Paul Thomas
    Article
    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    226961-plasmid-map-sequence-id-450824

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  12. SaCas9 GFP V3

    Plasmid
    #226962
    Purpose
    CBh-SaCas9-2A-GFP, and hU6-sgRNA (Sa) with a BbsI golden gate cloning backbone for sgRNA. sgRNA uses 3TC scaffold for increased sgRNA expression.
    Depositor
    Paul Thomas
    Article
    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    226962-plasmid-map-sequence-id-450821

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  13. DG SaCas9 puro V3

    Plasmid
    #226960
    Purpose
    CBh-SaCas9-2A-Puro, and 2X hU6-sgRNA (Sa) with BbsI golden gate cloning backbone for dual gRNAs. sgRNA uses 3TC scaffold for increased sgRNA expression.
    Depositor
    Paul Thomas
    Article
    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    226960-plasmid-map-sequence-id-450810

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  14. pTFG027_Spike_sACE2-resistant_D614G_F486S_3xFlag

    Plasmid
    #191572
    Purpose
    Mutated SARS-CoV-2 Spike under CMV with D614G, C-term 19 amino acid truncation, and 3x FLAG. Encodes F486S mutation. For expression and pseudotyping lentiviral particles.
    Depositor
    Joshua Leonard
    Article
    Gunnels et al Small. 2022 May;18(19):e2200125. doi: 10
    Insert
    SARS-CoV-2 Spike Protein (S Synthetic)
    Use
    Tags
    3x FLAG
    Expression
    Mammalian
    Mutation
    D614G and F486S (confers reduced binding affinity…
    Promoter
    CMV
    Available since
    Aug. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    191572-plasmid-map-sequence-id-441975

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  15. pBP-C_sacB

    Plasmid
    #204113
    Purpose
    Type 0 CDS part
    Depositor
    Mikhail Shapiro
    Insert
    Toxic levansucrase sacB
    Use
    Tags
    Expression
    Bacterial
    Mutation
    None
    Promoter
    Available since
    Jan. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    204113-plasmid-map-sequence-id-418236

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  19. pcDNA3-sACE2v5

    Plasmid
    #145152
    Purpose
    Mammalian expression plasmid for soluble ACE2 (protease domain) high affinity variant 5
    Depositor
    Erik Procko
    Article
    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.
    Insert
    Angiotensin-converting enzyme 2 (ACE2 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Extracellular, soluble protease domain (a.a. M1-D…
    Promoter
    CMV
    Available since
    April 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    145152-plasmid-map-sequence-id-292590

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  20. pcDNA3-sACE2v3

    Plasmid
    #145150
    Purpose
    Mammalian expression plasmid for soluble ACE2 (protease domain) high affinity variant 3
    Depositor
    Erik Procko
    Article
    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.
    Insert
    Angiotensin-converting enzyme 2 (ACE2 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Extracellular, soluble protease domain (a.a. M1-D…
    Promoter
    CMV
    Available since
    April 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    145150-plasmid-map-sequence-id-292586

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Showing: 61 - 80 of 8975 results