We narrowed to 10,998 results for: ENA;
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Plasmid#219749PurposeDVK_AF CIDAR vector encoding hispidin-synthase from Mycena citricolor under control of CMV promoter, for mammalian expressionDepositorInsertpCMV - mcitHispS - tSV40
UseTagsExpressionMammalianMutationPromoterAvailable SinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV Syn hBE Rh Guanylyl Cyclase1 2A tDimer
Plasmid#66779PurposeThe rhodopsin-guanylyl cyclase of the aquatic fungus Blastocladiella emersonii enables fast optical control of cGMP signalingDepositorInsertshbeRhGC
red fluorescent protein
UseAAVTagsExpressionMammalianMutationPromoterhuman SynapsinAvailable SinceOct. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
MtPT4-p3
Plasmid#160003PurposeMultigene construct containing the betalain pigment biosynthetic genes under the control of the MtPT4 promoter for visualisation of arbuscular mycorrhizal colonisation in Medicago truncatula roots.DepositorInsertsDsRed (reverse orientation)
DODAα1
CYP76AD1
cDOPA5GT
UseTagsExpressionPlantMutationPromoterArabidopsis thaliana Ubiquitin 10 Promoter (AtUb1…Available SinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFB-U6-Chrna5-gRNA-hSyn-mCherry-WPRE-SV40pA
Plasmid#128340PurposepAAV encoding gRNA sequence for loss-of-function indelsDepositorAvailable SinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-S586A-VLCAD-FLAG
Plasmid#55824PurposeExpresses S586A VLCAD mutant in mammalian cellsDepositorInsertVery long-chain specific acyl-CoA dehydrogenase (ACADVL Human)
UseTagsFLAG tagExpressionMammalianMutationchanged Serine 586 toAlaninePromoterCMVAvailable SinceJuly 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAR-9.47_VP2_AS
Plasmid#74241PurposeOriginal plasmid described in Choudhury et al, 2015 Mol. Ther. for production of AAV-AS vectors. Encodes VP2_AS chimeric protein with 19 alanines fused to the N-terminus of AAV9.47 VP2.DepositorInsertsAAV2 Rep
AAV9.47-VP2_AS
UseAAVTags19 alanines fused to VP2 of AAV9.47ExpressionMutationPromoterAvailable SinceApril 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-nAChr-Alpha6
Plasmid#48812Purposethis report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRFDepositorAvailable SinceApril 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET21b-LOV-ipaA L514K L531E
Plasmid#40253DepositorInsertLOV-ipaA L514K L531E
UseTags6xHisExpressionBacterialMutationL514K; L531E; Jα helix region hybridized (AsLOV2 …PromoterT7Available SinceSept. 4, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1-nAChr-beta3
Plasmid#48811PurposeThis report is the first to directly measure nAChR subunit stoichiometry using FRET and plasma membrane localization of α6- and β3-containing receptors using TIRF.DepositorInsertnAChr Beta 3 subunit, isoform 1 (Chrnb3 Mouse)
UseTagsExpressionMammalianMutation*PromoterCMVAvailable SinceSept. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
C-His-GvpC-Calpain
Plasmid#153295PurposeExpresses calpain-sensitive variant of Anabaena flos-aquae GvpC in E.coliDepositorInsertCalpain-sensitive Gas vesicle protein C (his-tagged)
UseTagsHis-tagExpressionBacterialMutation1) Contains an extra glycine after the methionine…PromoterT7Available SinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
delNC C-His Ana GvpC in pET28a
Plasmid#85736PurposeExpresses a truncated His-tagged GvpC from Anabaena flos-aquae without the N-and C-terminal regions in E.coliDepositorInsertGas vesicle protein C (truncated and his-tagged)
UseTagsHis-tagExpressionBacterialMutationDeletion of N and C terminal regions, addition of…PromoterT7Available SinceMarch 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFB-U6-Chrna3-gRNA-hSyn-mCherry-WPRE-SV40pA
Plasmid#128338PurposepAAV encoding gRNA sequence for loss-of-function indelsDepositorAvailable SinceJuly 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFB-U6-Chrna2-gRNA-hSyn-mCherry-WPRE-SV40pA
Plasmid#128337PurposepAAV encoding gRNA sequence for loss-of-function indelsDepositorAvailable SinceJuly 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFB-U6-Chrna4-gRNA-hSyn-mCherry-WPRE-SV40pA
Plasmid#128339PurposepAAV encoding gRNA sequence for loss-of-function indelsDepositorAvailable SinceJuly 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFB-U6-Chrnb3-gRNA-hSyn-mCherry-WPRE-SV40pA
Plasmid#128344PurposepAAV encoding gRNA sequence for loss-of-function indelsDepositorAvailable SinceJuly 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCyno-AAVS1-TetOn-ZIM3-KRAB-dCas9-P2A-mCherry
Plasmid#212830PurposeDoxycycline-inducible (Zim3)KRAB-dCas9-HA-P2A-mCherry cassette for integration at the AAVS1-locus of the cynomolgus genome.DepositorInsertZIM3-KRAB-dCas9-P2A-mCherry
UseTagsHA-tag and ZIM3-KRABExpressionBacterial and MammalianMutationPromoterCAG, TetOnAvailable SinceJune 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGorilla-AAVS1-TetOn-ZIM3-KRAB-dCas9-P2A-mCherry
Plasmid#212831PurposeDoxycycline-inducible (Zim3)KRAB-dCas9-HA-P2A-mCherry cassette for integration at the AAVS1-locus of the gorilla genome.DepositorInsertZIM3-KRAB-dCas9-P2A-mCherry
UseTagsHA-tag and ZIM3-KRABExpressionBacterial and MammalianMutationPromoterCAG, TetOnAvailable SinceJune 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pXPR_068
Plasmid#164555PurposeLentiviral vector that enables Flp-mediated sgRNA expression. Also encodes the fluorophore violet-excited GFP (Vex) as a marker of transduction.DepositorInsertsgRNA cassette
UseCRISPR and Lentiviral; Flp/frtTagsExpressionMammalianMutationPromoterHuman U6Available SinceNov. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFB-U6-Chrna6-gRNA-hSyn-mCherry-WPRE-SV40pA
Plasmid#128341PurposepAAV encoding gRNA sequence for loss-of-function indelsDepositorAvailable SinceJuly 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDF1-ADAMTS18 (Q1002X)
Plasmid#29557DepositorInsertADAMTS18 (ADAMTS18 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationchanged glutamine 1002 to stop codonPromoterAvailable SinceJuly 22, 2011AvailabilityAcademic Institutions and Nonprofits only