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Showing: 781 - 800 of 28680 results

  1. TRUPATH Triple Ga12

    Plasmid
    #196061
    Purpose
    Encodes a G alpha subunit (GNA12) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins
    Depositor
    Justin English
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Inserts
    GAlpha12-RLuc8 (GNA12 Human)
    GGamma9-GFP2
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    196061-plasmid-map-sequence-id-385874

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  2. pLenti6.2-ccdB-Nanoluc

    Plasmid
    #87075
    Purpose
    Lentiviral Gateway-compatible expression vector backbone with a C-terminal Nanoluc luciferase
    Depositor
    Mikko Taipale
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Nanoluc luciferase
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    87075-plasmid-map-sequence-id-215540

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  3. pLEW-pHluorin2-PTS1

    Plasmid
    #213776
    Purpose
    Expresses the fluorescent protein pH sensor pHlorin2 in the glycosomes of Trypanosomal brucei
    Depositor
    Kenneth Christensen
    Insert
    pHlorin2
    Use
    Trypanosoma brucei expression
    Tags
    Expression
    Mutation
    Appended a 2 glycine linker and a T. brucei perox…
    Promoter
    T. brucei rRNA promotor
    Available since
    Feb. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    213776-plasmid-map-sequence-id-423197

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  7. pLenti-mIFT88-EGFP

    Plasmid
    #149697
    Purpose
    Expresses mouse IFT88-EGFP in mammalian cells
    Depositor
    Ken-Ichi Takemaru
    Insert
    IFT88 (Ift88 Mouse)
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mutation
    Promoter
    EF1 alpha
    Available since
    Sept. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    149697-plasmid-map-sequence-id-307392

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  10. TRUPATH Triple Gai3

    Plasmid
    #196050
    Purpose
    Encodes a G alpha subunit (GNAl3) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins
    Depositor
    Justin English
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Inserts
    GAlphai3-RLuc8 (GNAI3 Human)
    GGamma9-GFP2
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    196050-plasmid-map-sequence-id-385864

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  11. pLEX307-DPP4-blast

    Plasmid
    #158452
    Purpose
    Constitutive lentiviral expression of human DPP4.
    Depositor
    Sho Iketani, Alejandro Chavez
    Insert
    DPP4 (DPP4 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    158452-plasmid-map-sequence-id-308162

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  12. mApple-LAMP1-phLuorin-N-8

    Plasmid
    #54918
    Purpose
    Localization: Lysosome Membrane, Excitation: 568, Emission: 592
    Depositor
    Michael Davidson
    Insert
    LAMP1 phLuorin
    Use
    Tags
    mApple
    Expression
    Mammalian
    Mutation
    Lysosomes-20 double labeled with phLuorin
    Promoter
    CMV
    Available since
    Aug. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    54918-plasmid-map-sequence-id-372787

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  13. plex_307-ccdB-blasticidin

    Plasmid
    #201136
    Purpose
    pLEX_307 plasmid with blasticidin marker
    Depositor
    Alejandro Chavez
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1α
    Available since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    201136-plasmid-map-sequence-id-395507

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  14. pLenti-mCherry-CAAX

    Plasmid
    #129285
    Purpose
    Lentiviral plasmid for expression of fluorescent reporter mCherry targeted to the plasma membrane via CAAX polybasic sequence
    Depositor
    Carlos Carmona-Fontaine
    Article
    Janska et al Dis Model Mech. 2021 Aug 1;14(8). pii: 2
    Insert
    Fluorescent reporter mCherry fused to CAAX polybasic sequence
    Use
    Lentiviral
    Tags
    CAAX
    Expression
    Mammalian
    Mutation
    Promoter
    CMV promoter
    Available since
    June 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    129285-plasmid-map-sequence-id-252373

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  16. plex_306-ccdB-hygro

    Plasmid
    #201135
    Purpose
    pLEX_306 plasmid with hygro marker
    Depositor
    Alejandro Chavez
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    SV40
    Available since
    June 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    201135-plasmid-map-sequence-id-396789

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  17. TRUPATH Triple Ga15

    Plasmid
    #196060
    Purpose
    Encodes a G alpha subunit (GNA15) with RLuc8, a G gamma subunit (GNG13) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins
    Depositor
    Justin English
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Inserts
    GAlpha15-RLuc8 (GNA15 Human)
    GGamma13-GFP2 (GNG13 Human)
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    196060-plasmid-map-sequence-id-385870

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  18. pLenti-PE2-BSD

    Plasmid
    #161514
    Purpose
    Expression of PE2 in mammalian cells. Can be used to generate stable cell lines by Blasticidin selection
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Biotechnol. 2020 Sep 21. pii: 10.103
    Insert
    PE2-P2A-BSD
    Use
    Lentiviral
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1α core promoter
    Available since
    Nov. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    161514-plasmid-map-sequence-id-317692

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  19. LLP773_pLenti-6xHRBKET-sgRNA

    Plasmid
    #211766
    Purpose
    Multiplexed gRNA plasmid targeting six different gene promoters (PACC1-2, EPCAM-2, B2M-4, KL-3, RBM3-1, HINT1-1), with mCherry selection
    Depositor
    Ryan Lister
    Article
    Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.
    Insert
    gRNAs against six different human gene promoters (PACC1-2, EPCAM-2, B2M-4, KL-3, RBM3-1, HINT1-1)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    pCMV and U6
    Available since
    Dec. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    211766-plasmid-map-sequence-id-417396

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  20. pLEX307-TMPRSS2-puro

    Plasmid
    #158457
    Purpose
    Constitutive lentiviral expression of human TMPRSS2.
    Depositor
    Sho Iketani, Alejandro Chavez
    Insert
    TMPRSS2 (TMPRSS2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    158457-plasmid-map-sequence-id-324267

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Showing: 781 - 800 of 28680 results