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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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Showing: 781 - 800 of 250849 results

  1. pTF258 (pFA6a-PSc-6xGly-His3MX)

    Plasmid
    #44062
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    (XhoI-KpnI-XbaI)-containing spacer, 6xGly linker,…
    Available Since
    April 18, 2013
    Availability
    Academic Institutions and Nonprofits only
    44062-plasmid-map-sequence-id-61661

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  2. pTF261 (pFA6a-TEV-6xGly-HphMX)

    Plasmid
    #44080
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    (XhoI-KpnI-XbaI)-containing spacer, 6xGly linker,…
    Available Since
    April 2, 2013
    Availability
    Academic Institutions and Nonprofits only
    44080-plasmid-map-sequence-id-61664

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  3. pTF262 (pFA6a-PSc-6xGly-HphMX)

    Plasmid
    #44074
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    (XhoI-KpnI-XbaI)-containing spacer, 6xGly linker,…
    Available Since
    April 2, 2013
    Availability
    Academic Institutions and Nonprofits only
    44074-plasmid-map-sequence-id-61665

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  4. pTF259 (pFA6a-TEV-6xGly-KanMX)

    Plasmid
    #44092
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    (XhoI-KpnI-XbaI)-containing spacer, 6xGly linker,…
    Available Since
    April 2, 2013
    Availability
    Academic Institutions and Nonprofits only
    44092-plasmid-map-sequence-id-61662

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  5. PP-MAPK2 (3RP9)

    Plasmid
    #32862
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    MAPK2
    Tags
    His tag with TEV cleavage
    Expression
    Bacterial
    Mutation
    amino acids 123-548 only, P421L
    Available Since
    Jan. 10, 2013
    Availability
    Industry, Academic Institutions, and Nonprofits

  6. MPP8 (3R93)

    Plasmid
    #32050
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    MPP8 (MPHOSPH8 Human)
    Tags
    His (with TEV cleavage)
    Expression
    Bacterial
    Available Since
    Jan. 6, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  7. integrin beta6 Y762F pBabe puro

    Plasmid
    #13597
    Depositor
    Dean Sheppard
    Insert
    integrin beta 6 Y762F (ITGB6 Human)
    Use
    Retroviral
    Expression
    Mammalian
    Mutation
    Y762F
    Available Since
    Jan. 8, 2007
    Availability
    Academic Institutions and Nonprofits only

  8. FOR human NANOS3 TALEN

    Plasmid
    #59722
    Purpose
    TALEN expressing vector used to allow Knock-in of NANOS3-P2A-mCherry knockin donor plasmid (addgene #59721)
    Depositor
    Jacob Hanna
    Article
    Irie et al Cell. 2015 Jan 15;160(1-2):253-68. doi:
    Insert
    HD HD NG NH HD NG HD NG HD HD HD NG HD HD NI NG
    Use
    TALEN
    Expression
    Mammalian
    Promoter
    CAGGS
    Available Since
    Dec. 29, 2014
    Availability
    Academic Institutions and Nonprofits only

  9. REV human NANOS3 TALEN

    Plasmid
    #59723
    Purpose
    TALEN expressing vector used to allow Knock-in of NANOS3-P2A-mCherry knockin donor plasmid (addgene #59721)
    Depositor
    Jacob Hanna
    Article
    Irie et al Cell. 2015 Jan 15;160(1-2):253-68. doi:
    Insert
    NG NH HD HD HD NI HD HD NG NH NG NI NH NH HD NI
    Use
    TALEN
    Expression
    Mammalian
    Promoter
    CAGGS
    Available Since
    Dec. 29, 2014
    Availability
    Academic Institutions and Nonprofits only

  10. VVT1

    Plasmid
    #65779
    Purpose
    The Joung Lab recommends using BPK2660 (Addgene plasmid 70709) instead of VVT1 as guide RNAs cloned into BPK2660 are more effective than this plasmid. Human expression plasmid for S. aureus Cas9 sgRNA
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    SaCas9 gRNA backbone, without spacer sequence
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    June 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    65779-plasmid-map-sequence-id-112660

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  14. pTFAP2B.1.0-gDNA

    Plasmid
    #113791
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor TFAP2B
    Depositor
    Kevin White
    Insert
    TFAP2B (TFAP2B Human)
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    113791-plasmid-map-sequence-id-230594

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  15. AAV pCAG-FLEX-mScarlet-WPRE

    Plasmid
    #99280
    Purpose
    Can be used to generate AAV virus that will express mScarlet in the presence of Cre
    Depositor
    Rylan Larsen
    Insert
    mScarlet
    Use
    AAV and Cre/Lox
    Available Since
    Aug. 18, 2017
    Availability
    Academic Institutions and Nonprofits only
    99280-plasmid-map-sequence-id-487711

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  16. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-EGFP

    Plasmid
    #118156
    Purpose
    Catalytically inactive Cas9 from S. pyogenes with P2A-EGFP under the EF1a core promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Jorge Ferrer
    Insert
    KRAB-dCas9-P2A-EGFP
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Promoter
    EF1a core and U6
    Available Since
    April 1, 2019
    Availability
    Academic Institutions and Nonprofits only
    118156-plasmid-map-sequence-id-239644

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  18. pLenti-MetGFP

    Plasmid
    #37560
    Depositor
    David Rimm
    Insert
    MET (MET Human)
    Use
    Lentiviral
    Tags
    GFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    July 13, 2012
    Availability
    Academic Institutions and Nonprofits only
    37560-plasmid-map-sequence-id-339875

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  19. pNR4A1.1.0-gDNA

    Plasmid
    #112391
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor NR4A1
    Depositor
    Kevin White
    Insert
    NR4A1 (NR4A1 Human)
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Dec. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    112391-plasmid-map-sequence-id-230237

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Showing: 781 - 800 of 250849 results