We narrowed to 162,191 results for: addgene

1. pMpGWB-327-Ef1-MpCKA-K151M-D258N-TagRFP

   Plasmid

   #238530

   Purpose

   Plant expression of M. polymorpha CK2 alpha K151M, D258N, tagged with TagRFP in plants

   Depositor

   Michael Hothorn

   Insert

   MpCKA
   

   Use

   Tags

   TagRFP

   Expression

   Plant

   Mutation

   K151M, D258N

   Promoter

   Available since

   June 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. NL63_NSP13_BV

   Plasmid

   #234380

   Purpose

   Baculovirus expression for structure determination. May not contain entire coding region of gene.

   Depositor

   Cheryl Arrowsmith

   Insert

   Nsp13_NL63
   

   Use

   Baculovirus expresssion

   Tags

   MHHHHHHSSGRENLYFQG

   Expression

   Mutation

   Promoter

   Polyhedrin

   Available since

   April 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHR-mCh-Cry2WT_NLS

   Plasmid

   #221925

   Purpose

   Expression of SV40 nuclear localization signal (NLS) fused to optogenetic protein mCh-Cry2WT

   Depositor

   Daniel Bose

   Insert

   SV40 NLS
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   insertion of SV40 NLS

   Promoter

   Available since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. PATL2_G288-Y543_BV_his

   Plasmid

   #194760

   Depositor

   Cheryl Arrowsmith

   Insert

   PATL2 (PATL2 Human)
   

   Use

   Baculovirus expression

   Tags

   Expression

   Mutation

   Promoter

   Polyhedrin

   Available since

   July 19, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pLV Nup88-V5

   Plasmid

   #175117

   Purpose

   Lentiviral expression of mouse Nup88-V5

   Depositor

   Larry Gerace

   Insert

   Nup88 (Nup88 Mouse)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLV Rbmxl1-V5

   Plasmid

   #175124

   Purpose

   Lentiviral expression of mouse Rbmxl1-V5

   Depositor

   Larry Gerace

   Insert

   Rbmxl1 (Rbmxl1 Mouse)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRARB.1.0-gDNA

   Plasmid

   #132471

   Purpose

   CRISPR/Cas9 plasmid to create GFP fusion proteins

   Depositor

   Kevin White

   Insert

   RARB (RARB Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. 6NEY

   Plasmid

   #127295

   Purpose

   Bacterial expression for structure determination. May not contain entire coding region of gene

   Depositor

   Cheryl Arrowsmith

   Insert

   PP-BRD3
   

   Use

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 26, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. 6MF9

   Plasmid

   #127290

   Purpose

   Bacterial expression for structure determination. May not contain entire coding region of gene

   Depositor

   Cheryl Arrowsmith

   Insert

   PP-BRD12
   

   Use

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 18, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pUPD1:P1-MAR

    Plasmid

    #106213

    Purpose

    Provides Glycine max P1 matrix attachment region as a level 0 GoldenBraid part

    Depositor

    Tomáš Moravec

    Insert

    Glycine max P1 matrix attachment region
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. 5CBD

    Plasmid

    #69970

    Purpose

    Baculovirus expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    PVX_084705:MAC04G-C11:C228845
    

    Use

    Baculovirus expression

    Tags

    His and TEV cleavage site

    Expression

    Mutation

    Promoter

    polyhedrin

    Available since

    Oct. 7, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

12. 5C4Q

    Plasmid

    #69963

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    LdBPK_363130.1:MAC055-F03:C235029
    

    Use

    Tags

    His and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lacO

    Available since

    Oct. 7, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

13. XE174 Xenopus Wnt-5A-myc-pCDbeta-G

    Plasmid

    #16873

    Depositor

    Randall Moon

    Insert

    Wnt-5A (wnt5a.S Frog)
    

    Use

    Xenopus expression

    Tags

    myc

    Expression

    Mutation

    Promoter

    Available since

    March 26, 2013

    Availability

    Academic Institutions and Nonprofits only

14. XE176 Xenopus Wnt-8-myc-pCDbeta-G

    Plasmid

    #16864

    Depositor

    Randall Moon

    Insert

    Wnt-8 (wnt8a.L Frog)
    

    Use

    Xenopus expression

    Tags

    myc

    Expression

    Mutation

    Promoter

    Available since

    March 14, 2008

    Availability

    Academic Institutions and Nonprofits only

15. ShuttleNSE

    Plasmid

    #50958

    Purpose

    A backbone for adenovirus creation by the AdEasy method, similar to ShuttleCMV (Addgene Plasmid # 16403), except it uses a Neuron-specific enolase (NSE) promoter instead of the CMV promoter

    Depositor

    James Bamburg

    Article

    Mi et al PLoS One. 2013 Dec 31;8(12):e83609. doi:

    Type

    Empty backbone

    Use

    Adenoviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    NSE

    Available since

    Feb. 20, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pLG1-puro Non-targeting sgRNA 3

    Plasmid

    #109003

    Purpose

    CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

    Depositor

    Jacob Corn

    Article

    Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

    Insert

    Non-targeting sgRNA #3
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6 promoter

    Available since

    Aug. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLG1-puro Non-targeting sgRNA 1

    Plasmid

    #109002

    Purpose

    CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

    Depositor

    Jacob Corn

    Article

    Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

    Insert

    Non-targeting sgRNA #1
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6 promoter

    Available since

    Aug. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. Oct4-Cr1

    Plasmid

    #66989

    Purpose

    Expresses gRNA that targets stop codon for OCT4

    Depositor

    Danwei Huangfu

    Article

    Zhu et al Stem Cell Reports. 2015 May 28. pii: S22

    Insert

    gRNA for Crispr targeting the OCT4 stop codon
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 22, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pGL3-U6-sgRNA-PGK-puromycin-AflII

    Plasmid

    #106404

    Purpose

    Empty guide RNA cloning vector with an AflII site added from Addgene 41824

    Depositor

    Jason Gertz

    Article

    Carleton et al Cell Syst. 2017 Oct 25;5(4):333-344.e5.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1-IARS2-H112A/P115A

    Plasmid

    #232338

    Purpose

    Expresses human IARS2 with putative isoleucine binding deficiency mutations (H112A and P115A) in mammalian cells, also carrying synonymous mutations described in Addgene #232340

    Depositor

    Quanfu Ma

    Insert

    IARS2 (IARS2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Putative isoleucine binding deficiency mutation H…

    Promoter

    CMV

    Available since

    June 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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