We narrowed to 1,235 results for: sumo

1. PIAS2

   Plasmid

   #40743

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   PIAS2 (PIAS2 )
   

   Use

   Tags

   6xHis TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 18, 2012

   Availability

   Industry, Academic Institutions, and Nonprofits

2. TBXTA-c021

   Plasmid

   #139758

   Purpose

   Protein expression/crystallography (PDB:5QT0)

   Depositor

   Opher Gileadi

   Insert

   TBXT, DNA-binding domain, G177D (TBXT Human)
   

   Use

   Tags

   His6-SUMO

   Expression

   Bacterial

   Mutation

   G177D; contains only amino acids E41-N211

   Promoter

   T7

   Available since

   May 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Coxsackievirus B3 (CVB3) 3C protease isolate KR-2002

   Plasmid

   #228639

   Purpose

   Bacterial expression of the Coxsackievirus 33 isoform B 3C protease

   Depositor

   ASAP Discovery, Frank von Delft

   Insert

   Coxsackievirus 33 isoform B 3C protease
   

   Use

   Tags

   His-SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 14, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. Enterovirus A71 (EV-A71) isolate TW-2553-1998 3C protease

   Plasmid

   #228636

   Purpose

   Bacterial expression of the A71 3C protease (isoform B) used in crystallisation

   Depositor

   ASAP Discovery, Frank von Delft

   Insert

   Enterovirus A71 3C protease isoform B
   

   Use

   Tags

   His-SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 14, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. Echovirus 11 (E11 3C) protease A isolate E11-PLA-NECK-23-E0-62-P1-FRA23

   Plasmid

   #228645

   Purpose

   Bacterial expression of the E11 3C protease

   Depositor

   ASAP Discovery, Frank von Delft

   Insert

   Echo virus 11 3C protease
   

   Use

   Tags

   His-SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 18, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. Coxsackievirus B4 (CVB4) 3C protease isolate CVB4/USA/9R1/2012

   Plasmid

   #228640

   Purpose

   Bacterial expression of the Coxsackievirus 43 isoform B 3C protease

   Depositor

   ASAP Discovery, Frank von Delft

   Insert

   Coxsackievirus 43 isoform B 3C protease
   

   Use

   Tags

   His-SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 18, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. CNN3A-c002

   Plasmid

   #175456

   Purpose

   Protein expression in bacterial cells. Entire CDS, M1-Y329.

   Depositor

   Opher Gileadi

   Insert

   CNN3 (CNN3 Human)
   

   Use

   Tags

   His6-SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. CNN3A-c007

   Plasmid

   #175457

   Purpose

   Protein expression in bacterial cells. Lacking N-terminal 10 residues, G11-Y329.

   Depositor

   Opher Gileadi

   Insert

   CNN3 (CNN3 Human)
   

   Use

   Tags

   His6-SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET29a-human histone 4-in-One

   Plasmid

   #223237

   Purpose

   Polycistronic bacterial coexpression construct for human His-Sumo-H2A, H2B, H3, and Dual-StrepII-H4

   Depositor

   Weixing Zhao

   Inserts

   His6-VSVG-Histone H2A
   Histone H2B
   Histone H3
   Histone H4-TEV-His6-Dual StrepII
   

   Use

   Tags

   Dual StrepII, His6, TEV protease site, and VSVG

   Expression

   Bacterial

   Mutation

   A103G

   Promoter

   T7

   Available since

   Aug. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pALS3-Ma-sfGFP-WT

    Plasmid

    #212119

    Purpose

    Fluorescence control plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses sfGFP-WT and M. alvus Pyl-tRNA(6). p15a origin. Tetracycline resistance.

    Depositor

    Ryan Mehl

    Inserts

    SUMO-sfGFP-WT
    M. alvus Pyl-tRNA(6)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    araC and lpp

    Available since

    Sept. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Cys-3HB-SPYcatcher

    Plasmid

    #214838

    Purpose

    This plasmid encodes a protein (Cys-3HB-SPYcatcher) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.

    Depositor

    Hironori Funabiki

    Article

    Arimura et al bioRxiv 2024.01.21.576499.

    Insert

    Cys-3HB-SPYcatcher
    

    Use

    Tags

    His-TEV-SUMO-

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Cys-60nmSAH-SPYcatcher

    Plasmid

    #214840

    Purpose

    This plasmid encodes a protein (Cys-60nmSAH-SPYcatcher) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.

    Depositor

    Hironori Funabiki

    Article

    Arimura et al bioRxiv 2024.01.21.576499.

    Insert

    Cys-60nmSAH-SPYcatcher
    

    Use

    Tags

    His-TEV-SUMO-

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. SPYtag-GFPnanobody

    Plasmid

    #214837

    Purpose

    This plasmid encodes a protein (SPYtag-GFPnanobody) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.

    Depositor

    Hironori Funabiki

    Article

    Arimura et al bioRxiv 2024.01.21.576499.

    Insert

    SPYtag-GFPnanobody
    

    Use

    Tags

    His-TEV-SUMO-

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Cys-30nmSAH-SPYcatcher

    Plasmid

    #214839

    Purpose

    This plasmid encodes a protein (Cys-30nmSAH-SPYcatcher) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.

    Depositor

    Hironori Funabiki

    Article

    Arimura et al bioRxiv 2024.01.21.576499.

    Insert

    Cys-30nmSAH-SPYcatcher
    

    Use

    Tags

    His-TEV-SUMO-

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. DENV2 NS2B-NS3 protease: Co-expression of DENV-2 NS2B and NS3

    Plasmid

    #204808

    Purpose

    Bacterial co-expression of Dengue2 NS2B and NS3 proteins

    Depositor

    ASAP Discovery, Frank von Delft

    Insert

    Dengue2 NS2B (POLY Dengue2)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 18, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. 6CKO (DOT1L)

    Plasmid

    #119962

    Purpose

    Bacterial expression for structure determination

    Depositor

    Cheryl Arrowsmith

    Insert

    DOT1L (DOT1L Human)
    

    Use

    Tags

    6xHIS, thrombin,SUMO and TEV cleavage

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 1, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. WNV NS2B-NS3 protease: Co-expression of NS2B and NS3

    Plasmid

    #204806

    Purpose

    Bacterial co-expression of West Nile NS2B and NS3 proteins

    Depositor

    ASAP Discovery, Frank von Delft

    Insert

    West Nile NS2B
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 18, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pSF1389

    Plasmid

    #104962

    Purpose

    Bacterial expression plasmid of bdSENP1 protease

    Depositor

    Dirk Görlich

    Article

    Frey et al J Chromatogr A. 2014 Apr 11;1337:95-105.

    Insert

    SENP1
    

    Use

    Tags

    His14 tag and Tev protease cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    lacUV5

    Available since

    Jan. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. 6CCR (GID4)

    Plasmid

    #119960

    Purpose

    Bacterial expression for structure determination

    Depositor

    Cheryl Arrowsmith

    Insert

    GID4 (C17orf39 Human)
    

    Use

    Tags

    6xHIS, thrombin,SUMO and TEV cleavage

    Expression

    Bacterial

    Mutation

    contains amino acids 116-300

    Promoter

    T7

    Available since

    Jan. 8, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pESC-URA-GFP-Ubc9wt

    Plasmid

    #20368

    Depositor

    Judith Frydman

    Article

    Kaganovich et al Nature. 2008 Aug 28. 454(7208):1088-95.

    Insert

    SUMO-conjugating enzyme Ubc9 (UBC9 Budding Yeast)
    

    Use

    Yeast/bacteria shuttle

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 28, 2009

    Availability

    Academic Institutions and Nonprofits only