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VRER variant

Plasmid

#138561

Purpose

Expresses human codon-optimized SpCas9-VRER and blasticidin resistance: EFS promoter-VRER-NLS-FLAG-P2A-BSD

Depositor

Hyongbum Kim

Article

Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

Insert

VRER

Use

CRISPR and Lentiviral

Tags

BSD, FLAG, and NLS

Expression

Mammalian

Mutation

D1135V, G1218R, R1335E, T1337R

Promoter

EFS

Available Since

June 9, 2020

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2puro-Ptgs2_Balbc

Plasmid

#170301

Purpose

A knock-out vector for the mouse Ptgs2 in Balb/c

Depositor

Michiyuki Matsuda

Article

Konishi et al Cancer Res. 2021 May 25. pii: 0008-5472.

Insert

A gRNA targeting the mouse tyrosinase gene.

Use

CRISPR and Lentiviral

Available Since

July 8, 2021

Availability

Academic Institutions and Nonprofits only

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pXW117As(RS-E11)

Plasmid

#123145

Purpose

Arsenic sensor with two-layered amplifier cascade. pSB4A3 carrying J117-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-31ECF11(ASV)-t-Pecf11-30gfp-t

Depositor

Baojun Wang

Article

Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

Insert

J117-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-31ECF11(ASV)-t-Pecf11-30gfp-t

Use

Synthetic Biology

Expression

Bacterial

Promoter

J117, ParsR, PhrpLE, Pecf11

Available Since

May 9, 2019

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2puro-Gnaq

Plasmid

#170302

Purpose

A knock-out vector for the mouse Gnaq

Depositor

Michiyuki Matsuda

Article

Konishi et al Cancer Res. 2021 May 25. pii: 0008-5472.

Insert

A gRNA targeting the mouse Gnaq gene.

Use

CRISPR and Lentiviral

Available Since

July 8, 2021

Availability

Academic Institutions and Nonprofits only

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pCarCBAE

Plasmid

#117755

Purpose

Codon optimized CarCBAE enzymes from Pectobacterium carotovorum in pBbaA5k backbone

Depositor

Greg Bokinsky

Article

Shomar et al Nat Chem Biol. 2018 Aug;14(8):794-800. d

Insert

Codon optimised Carbapenem synthase (CarC), Carboxymethylproline synthase (CarB), Carbapenam synthetase (CarA), Ferredoxin CarE

Expression

Bacterial

Promoter

PLac(UV5)

Available Since

Nov. 27, 2018

Availability

Academic Institutions and Nonprofits only

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WPXL LEFTY2 gRNA

Plasmid

#101924

Purpose

LEFTY2 gRNA used for targeted demethylation is inserted into the WPXL lentiviral backbone.

Depositor

Danwei Huangfu

Article

Verma et al Nat Genet. 2018 Jan;50(1):83-95. doi: 10

Insert

LEFTY2 enhancer targeting gRNA

Use

Lentiviral

Available Since

Dec. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pKD265

Plasmid

#125095

Purpose

Expression of torR(1-134aa)-psdR(134-237aa), ompR137 chimera under PLtetO-1, output Promoter PpsdA110

Depositor

Jeffrey Tabor

Article

Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

Inserts

torR(REC)-psdR(DBD)137
sfgfp
tetR

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pSR322

Plasmid

#125116

Purpose

Expression of uhpA(1-123aa)-ydfI(126-213aa), narL131 chimera under PLtetO-1, output Promoter PydfJ115

Depositor

Jeffrey Tabor

Article

Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

Inserts

uhpA(REC)-ydfI(DBD)131
sfgfp
tetR

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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pSR40.15

Plasmid

#125082

Purpose

Expression of ompR(1-137aa)-ccaR(127-234aa) chimera under PLtetO-1, output Promoter PcpcG2-172

Depositor

Jeffrey Tabor

Article

Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

Inserts

ompR(REC)-ccaR(DBD)137
sfgfp
tetR

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 23, 2019

Availability

Academic Institutions and Nonprofits only

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pSR40.29

Plasmid

#125088

Purpose

Expression of ompR(1-139aa)-ccaR(129-234aa) chimera under PLtetO-1, output Promoter PcpcG2-172

Depositor

Jeffrey Tabor

Article

Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

Inserts

ompR(REC)-ccaR(DBD)139
sfgfp
tetR

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 23, 2019

Availability

Academic Institutions and Nonprofits only

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sgMLST8_2

Plasmid

#124906

Purpose

Targeting human MLST8 gene by CRISPR-Cas9

Depositor

Jin Chen

Article

Hwang et al Cancer Res. 2019 Jul 1;79(13):3178-3184.

Insert

MLST8 (MLST8 Human)

Use

CRISPR and Lentiviral

Available Since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pHH0103_SH3RF2-3/3

Plasmid

#91281

Purpose

Protein expression and purification of human SH3 domain construct SH3RF2-3/3

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SH3RF2-3/3 (SH3RF2 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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WPXL SOX10 gRNA

Plasmid

#101923

Purpose

SOX10 gRNA used for targeted demethylation is inserted into the WPXL lentiviral backbone.

Depositor

Danwei Huangfu

Article

Verma et al Nat Genet. 2018 Jan;50(1):83-95. doi: 10

Insert

SOX10 promoter targeting gRNA

Use

Lentiviral

Available Since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

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CS2-Myc-mASUN

Plasmid

#47044

Purpose

Expression of tagged-mouse ASUN

Depositor

Laura Lee

Article

Jodoin et al Mol Biol Cell. 2012 Dec;23(24):4713-24.

Insert

Asunder (Asun Mouse)

Tags

Myc

Expression

Mammalian

Available Since

Sept. 10, 2013

Availability

Academic Institutions and Nonprofits only
Plxnc1-AP-His

Plasmid

#72005

Purpose

Expresses the extracellular region of the PlexinC1 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Plxnc1 (Plxnc1 Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only

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pCRII-Topo Diap3 in situ probe

Plasmid

#45602

Depositor

Jeffrey Macklis

Article

Arlotta et al Neuron. 2005 Jan 20;45(2):207-21.

Insert

Diap3 in situ probe (Diaph3 Mouse)

Use

In situ

Mutation

fragment contains bp# 1589-2321 of BC028920

Available Since

July 5, 2013

Availability

Academic Institutions and Nonprofits only
pCRII-Topo Crim1 in situ probe

Plasmid

#45600

Depositor

Jeffrey Macklis

Article

Arlotta et al Neuron. 2005 Jan 20;45(2):207-21.

Insert

Crim1 in situ probe (Crim1 Mouse)

Use

In situ

Mutation

insert contains bp#4062-4502 of NM_015800.3

Available Since

July 5, 2013

Availability

Academic Institutions and Nonprofits only
pJC2281 - pHR-SFFV-rtTA3-PGK-Bsr

Plasmid

#250247

Purpose

Expression of reverse tet transactivator for use in the Tet-On system.

Depositor

Jacob Corn

Article

Muhar et al Nature. 2025 Feb;638(8050):519-527. doi:

Insert

rtTA3

Use

Lentiviral

Promoter

SFFV

Available Since

Jan. 15, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV2-Sst44-mTagBFP2

Plasmid

#211807

Purpose

Expresses BFP in a subset of cortical somatostatin neurons.

Depositor

Christopher Harvey

Article

Green et al Nature. 2023 Aug;620(7973):366-373. doi:

Insert

mTagBFP2

Use

AAV

Tags

NLS, FLAG

Expression

Mammalian

Promoter

Sst44 enhancer, modified CMV promoter

Available Since

Jan. 5, 2026

Availability

Academic Institutions and Nonprofits only

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pEP0003_pIVTRup(BsmBI)_ABEmax

Plasmid

#242537

Purpose

IVT

Depositor

Christoph Bock, Paul Datlinger

Article

Datlinger et al Nature. 2025 Oct;646(8086):963-972. doi:

Insert

ABEmax (NGG-ABE)

Use

CRISPR

Mutation

D10A

Available Since

Nov. 21, 2025

Availability

Academic Institutions and Nonprofits only

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