We narrowed to 18,501 results for: MUT

1. pCW-ND-Cdt1-mCherry-Puro

   Plasmid

   #193766

   Purpose

   Doxycyline-inducible (TetOn) human Cdt1 (SV40 NLS localization and C-terminal mCherry tag) with mutations which abroggate its S phase degradation, expressed from all-in-one pCW vector (TetOn + rtTA)

   Depositor

   Tobias Meyer

   Article

   Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do

   Insert

   ND-Cdt1-mCherry (CDT1 Human)
   

   Use

   Lentiviral

   Tags

   mCherry

   Mutation

   amino acids 1-19 deleted, ΔCy mutation (aa68-70 t…

   Promoter

   TRE

   Available Since

   Jan. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCW57.1 ISCU D71A

   Plasmid

   #169880

   Purpose

   Expresses ISCU iron-sulfur cluster trapping mutant (dominant negative)

   Depositor

   Richard Possemato

   Article

   Terzi et al Sci Adv. 2021 May 26;7(22). pii: 7/22/ea

   Insert

   ISCU (ISCU Human, Synthetic)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   Codon Optimized, D71A iron-sulfur cluster trappin…

   Promoter

   TRE

   Available Since

   May 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. p(HA)HIF1alpha(401delta603)R27G

   Plasmid

   #52215

   Purpose

   mammalian expression of HA tagged HIF1alpha with a deletion of the N-terminal activation domain and R27G mutation

   Depositor

   Eric Huang

   Article

   Koshiji et al EMBO J. 2004 May 5;23(9):1949-56. Epub 2

   Insert

   HIF1alpha (401delta603) R27G (HIF1A Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   aa 401-603 deleted, removes the oxygen-dependent …

   Promoter

   CMV

   Available Since

   April 7, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pcDNA3-Lyso-PTEN(A4)-mCherry

   Plasmid

   #184054

   Purpose

   Lysosome-targeted constitutively active mutant of phosphatase and tensin homolog (PTEN); tagged with mCherry.

   Depositor

   Jin Zhang

   Article

   Chen et al ACS Cent Sci. 2021 Dec 22;7(12):2009-202

   Insert

   Lyso-PTEN(A4)-mCherry (PTEN Human)
   

   Tags

   Lysosome-associated membrane protein 1 (LAMP1). a…

   Expression

   Mammalian

   Mutation

   Ser 380/385 and Thr 382/383 in PTEN mutated to Al…

   Promoter

   CMV

   Available Since

   June 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDONR223_RIT1_WT

   Plasmid

   #82882

   Purpose

   Gateway Donor vector containing RIT1, part of the Target Accelerator Plasmid Collection.

   Depositor

   Jesse Boehm, Matthew Meyerson, David Root

   Article

   Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

   Insert

   RIT1 (RIT1 Human)
   

   Use

   Gateway entry vector

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pACEBac1-PARP1-Flag-His-7xKQ

   Plasmid

   #111573

   Purpose

   Expresses full length PARP1 with K to Q mutations in automodification domain in Sf9 (insect) cells

   Depositor

   Thomas Muir

   Article

   Liszczak et al Nat Chem Biol. 2018 Jul 16. pii: 10.1038

   Insert

   PARP1 (PARP1 Human)
   

   Tags

   C-terminal 6xHis and C-terminal Flag

   Expression

   Insect

   Mutation

   lysine to glutamine mutation at the following ami…

   Promoter

   polyhedrin promoter

   Available Since

   July 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDONR223_ZBTB24_WT

   Plasmid

   #82887

   Purpose

   Gateway Donor vector containing ZBTB24, part of the Target Accelerator Plasmid Collection.

   Depositor

   Jesse Boehm, Matthew Meyerson, David Root

   Article

   Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

   Insert

   ZBTB24 (ZBTB24 Human)
   

   Use

   Gateway entry vector

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti-puro/RAF1-P261A

   Plasmid

   #131728

   Purpose

   Mammalian Expression of RAF1

   Depositor

   Jacques De Grève

   Article

   Noeparast et al Oncogene. 2019 Aug;38(31):5933-5941. doi

   Insert

   RAF1 (RAF1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   P261A

   Promoter

   CMV

   Available Since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSLPB2B-FKBP12_F36V-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin

   Plasmid

   #187951

   Purpose

   FKBP12 (F36V mutant) degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.

   Depositor

   Edda Schulz

   Article

   Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

   Insert

   FKBP12_F36V-SpdCas9-tagRFPt-P2A-tagBFP
   

   Use

   CRISPR and Synthetic Biology

   Tags

   GGS linker

   Expression

   Mammalian

   Available Since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDONR223_NUP88_WT_V5

    Plasmid

    #83011

    Purpose

    Gateway Donor vector containing NUP88, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, Matthew Meyerson, David Root

    Article

    Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

    Insert

    NUP88 (NUP88 Human)
    

    Use

    Gateway entry vector

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHAGE-PIK3CB-E1051K

    Plasmid

    #116553

    Purpose

    Lentiviral expression of PIK3CB E1051K

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    PIK3CB (PIK3CB Human)
    

    Use

    Lentiviral

    Mutation

    E1051K

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHAGE-PIK3CG-E267K

    Plasmid

    #116577

    Purpose

    Lentiviral expression of PIK3CG E267K

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    PIK3CG (PIK3CG Human)
    

    Use

    Lentiviral

    Mutation

    E267K

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-EFNB2-RBD-Fc (D62Q-Q130L-V167L)

    Plasmid

    #200980

    Purpose

    Mammalian expression plasmid for soluble EFNB2 (D62Q-Q130L-V167L specificity mutant) fused to IgG1 Fc

    Depositor

    Erik Procko

    Article

    Narayanan et al J Virol. 2023 Nov 30;97(11):e0062123. do

    Insert

    EFNB2 (EFNB2 Human)
    

    Tags

    IgG1 Fc

    Expression

    Mammalian

    Mutation

    Soluble Receptor-Binding Domain; D62Q-Q130L-V167L…

    Promoter

    CMV

    Available Since

    Oct. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLgw EcoDam-V5-ELK1

    Plasmid

    #98604

    Purpose

    Mammalian DamID lentiviral vector for ELK1 with Dam-V5 using Gateway cloning

    Depositor

    Richard Harvey

    Article

    Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

    Insert

    ELK1 (Elk1 Mouse)
    

    Use

    Lentiviral; Damid

    Tags

    Dam (DNA adenine methyltransferase) and V5

    Expression

    Mammalian

    Promoter

    Heat Shock Minimal Promoter

    Available Since

    July 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. CMV-ShadowY-CBD(H83L/H86L)-P2A-Clover(T153M/F223R)-Cdc42

    Plasmid

    #165436

    Purpose

    Expresses ShadowY-CBD(H83L/H86L) and Clover(T153M/F223R)-Cdc42 in mammalian cells as a negative control for FLIM imaging.

    Depositor

    Hideji Murakoshi

    Article

    Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

    Insert

    ShadowY-CBD(H83L/H86L)-P2A-Clover(T153M/F223R)-Cdc42
    

    Tags

    ShadowY, Clover(T153M/F223R)

    Expression

    Mammalian

    Mutation

    H83L/H86L: CDB(Cdc42 Binding Domain, Pak3, residu…

    Promoter

    CMV

    Available Since

    March 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1-myc-AP1S3

    Plasmid

    #58292

    Purpose

    mammalian expression of AP1S3

    Depositor

    Francesca Capon

    Article

    Setta-Kaffetzi et al Am J Hum Genet. 2014 May 1;94(5):790-7.

    Insert

    AP1S3 (AP1S3 Human)
    

    Tags

    6xHis and myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 1, 2014

    Availability

    Academic Institutions and Nonprofits only

17. EIF2B3

    Plasmid

    #172072

    Purpose

    In vitro transcription for rescue experiments

    Depositor

    Cheol-Hee Kim

    Article

    Lee et al Hum Mol Genet. 2021 Apr 27;30(5):331-342

    Insert

    EIF2B3 (EIF2B3 Human)
    

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV

    Available Since

    July 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJV132

    Plasmid

    #124254

    Purpose

    Lentiviral vector (pRRL) containing KRAS G12V specific T cell receptor, clone 3

    Depositor

    Stanley Riddell

    Article

    Veatch et al Cancer Immunol Res. 2019 Jun;7(6):910-92

    Insert

    T cell receptor (KRAS Human)
    

    Use

    Lentiviral

    Promoter

    MSCV

    Available Since

    May 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLU-CMV-Flag-hATG5 E122D

    Plasmid

    #111804

    Purpose

    Mammalian Expression of hATG5

    Depositor

    Jun Hee Lee

    Article

    Kim et al Elife. 2016 Jan 26;5. pii: e12245. doi:

    Insert

    hATG5 (ATG5 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    E122D

    Available Since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDONR223_AKR1B1_WT

    Plasmid

    #82928

    Purpose

    Gateway Donor vector containing AKR1B1, part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, Matthew Meyerson, David Root

    Article

    Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

    Insert

    AKR1B1 (AKR1B1 Human)
    

    Use

    Gateway entry vector

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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