We narrowed to 20,110 results for: Cre

1. sgRNA scaffold-spacer-human U6

   Plasmid

   #214697

   Purpose

   provide "sgRNA scaffold-spacer-human U6" fragment

   Depositor

   Yin Shen

   Article

   Ren et al Mol Cell. 2023 Dec 21;83(24):4633-4645.e

   Insert

   sgRNA scaffold-spacer-human U6
   

   Use

   CRISPR

   Available Since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. mGFAP-Halo

   Plasmid

   #169474

   Purpose

   the host vector was obtained by substitution of the EGFP tag from a pEGFP-N3-mGFAP plasmid for a HaloTag using BamHI and NotI restriction sites

   Depositor

   Federico Herrera

   Article

   Letra-Vilela et al Experimental Results (2020), Volume 1, e

   Insert

   GFAP (Gfap Mouse)
   

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   Changed R 236 to H

   Available Since

   June 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pXPR_003 Intergenic control guide 2

   Plasmid

   #193585

   Purpose

   sgRNA control; induces CAS9 cutting in an intergenic region

   Depositor

   William Hahn

   Article

   Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

   Insert

   sgRNA control
   

   Use

   Lentiviral

   Available Since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pXPR_003 sgNFkB2 guide 2

   Plasmid

   #193594

   Purpose

   NFkB2 knockout

   Depositor

   William Hahn

   Article

   Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

   Insert

   sgNFkB2 guide 2 (NFKB2 Human)
   

   Use

   Lentiviral

   Available Since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGWB528

   Plasmid

   #74870

   Purpose

   Gateway cloning compatible binary vector for C-terminal fusion with TAP (no promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Expression

   Plant

   Available Since

   July 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pYSD153

   Plasmid

   #212929

   Purpose

   Encodes the mRuby2 fluorescent protein (Part3b') with the 111AA SCW4 TFP (Part3a') assembled with S. cerevisiae pTEF1 promoter, tTDH1 terminator, LEU2 selectable marker and CEN/ARS yeast origin.

   Depositor

   Paul Young

   Article

   O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

   Insert

   mRuby2
   

   Expression

   Yeast

   Available Since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pYSD141

   Plasmid

   #212928

   Purpose

   Encodes the mRuby2 fluorescent protein (Part3b') with the Aga2 signal peptide (Part3a') assembled with S. cerevisiae pTEF1 promoter, tTDH1 terminator, LEU2 selectable marker and CEN/ARS yeast origin.

   Depositor

   Paul Young

   Article

   O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

   Insert

   mRuby2
   

   Expression

   Yeast

   Available Since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. CD200RCd4d3+4-bio

   Plasmid

   #32406

   Purpose

   A positive control receptor bait (rat CD4d3+4-bio) and prey (rat CD200 receptor) EXPRESs plasmid for The Basic AVEXIS kit

   Depositor

   Gavin Wright

   Article

   Bushell et al Genome Res. 2008 Apr;18(4):622-30. Epub

   Insert

   CD200 Receptor extracellular domain (Cd200r1 Rat)
   

   Tags

   Cd4d3+4 and biotinylation peptide

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 17, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBabe puro RalA (long) wt

   Plasmid

   #19717

   Depositor

   Channing Der

   Article

   Lim et al Curr Biol. 2006 Dec 19. 16(24):2385-94.

   Insert

   V-ral simian leukemia viral oncogene homolog A (RALA Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   NOTE: long splice variant with 3bp deletion and 1…

   Available Since

   Oct. 27, 2008

   Availability

   Academic Institutions and Nonprofits only

10. pCMV-bArrestin2-TevC-P2A-TdTomato

    Plasmid

    #89869

    Purpose

    expresses iTango bArrestin2-TevC component and TdTomato bicistronically

    Depositor

    Hyungbae Kwon

    Article

    Lee et al Nat Methods. 2017 May;14(5):495-503. doi

    Insert

    bArrestin2-TevC-P2A-TdTomato
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Mutation

    R173H in TdTomato (please see depositor comments …

    Promoter

    CMV

    Available Since

    May 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. KN206: pMVP/eCFP-DEST

    Plasmid

    #121854

    Purpose

    pMVP destination vector, empty expression vector backbone w/ eCFP selection cassette

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Pmvp gateway destination vector

    Expression

    Mammalian

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pOCC267

    Plasmid

    #118887

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and N-terminal HALO tag

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HIS6-MBP-3C-HALO-NotI-ccdB-AscI-stop-HindIII cassette
    

    Tags

    HIS6-MBP, cleavable with 3C protease; HALO tag

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. CS2 Flag-Smad2 (100-467)

    Plasmid

    #14928

    Depositor

    Joan Massague

    Article

    Hata et al Nature. 1997 Jul 3. 388(6637):82-7.

    Insert

    Smad2 (100-467) (SMAD2 Human)
    

    Use

    Xenopus/mammalian/avian/zebrafish

    Tags

    Flag

    Mutation

    amino acids 100-467

    Available Since

    May 17, 2007

    Availability

    Academic Institutions and Nonprofits only

14. PAK4-FL-3'UTR

    Plasmid

    #113088

    Purpose

    Luciferase reporter containing PAK4- FL-3'UTR

    Depositor

    James Donahue

    Article

    Phatak et al Oncotarget. 2018 Jun 19;9(47):28391-2840

    Insert

    p21 Activated kinase 4 (PAK4 Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    none

    Promoter

    PGK

    Available Since

    Aug. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCDNAIIIB rhoA WT

    Plasmid

    #61631

    Purpose

    mammalian expression of rhoA

    Depositor

    JS Gutkind

    Article

    Coso et al Cell. 1995 Jun 30;81(7):1137-46.

    Insert

    rhoA (RHOA Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

16. CS2 Flag-Smad2 (1-185)

    Plasmid

    #14929

    Depositor

    Joan Massague

    Article

    Hata et al Nature. 1997 Jul 3. 388(6637):82-7.

    Insert

    Smad2 (1-185) (SMAD2 Human)
    

    Use

    Xenopus/mammalian/avian/zebrafish

    Tags

    Flag

    Mutation

    amino acids 1-185

    Available Since

    May 17, 2007

    Availability

    Academic Institutions and Nonprofits only

17. pLib6.4-Aalb_744

    Plasmid

    #176663

    Purpose

    Expression of sgRNA under Ae. albopictus U6 (AALF029744) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

    Depositor

    Norbert Perrimon

    Article

    Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

    Type

    Empty backbone

    Use

    Crispr

    Expression

    Insect

    Available Since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. DsRed2_pLENTI-CAG-IRES-GFP

    Plasmid

    #177010

    Purpose

    Mammalian lentiviral expression vector encoding DsRed2

    Depositor

    Roger Reeves

    Article

    Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

    Insert

    DsRed2
    

    Use

    Lentiviral

    Promoter

    CAG

    Available Since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTYF-sGFAP-LOx-IRES-tdTomato

    Plasmid

    #166919

    Purpose

    Express the bacterial lactate oxidase (LOx) enzyme in astrocytes

    Depositor

    Sergey Kasparov

    Article

    Vaccari Cardoso et al Glia. 2021 Jan 5. doi: 10.1002/glia.2396

    Insert

    Lactate oxidase
    

    Use

    Lentiviral

    Tags

    tdTomato

    Expression

    Mammalian

    Promoter

    sGFAP

    Available Since

    April 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDendra2-Hygro

    Plasmid

    #202407

    Purpose

    Dendra2 expression plasmid

    Depositor

    Morito Kurata

    Article

    Sugita et al Commun Biol. 2023 Jun 1;6(1):582. doi: 1

    Insert

    Hygromycin
    

    Tags

    Dendra2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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