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BASIC_8_RBS34- mCherry -B0015

Plasmid

#68142

Purpose

storage vector for BASIC part: RBS-RFP(ORF)

Depositor

Geoffrey Baldwin

Article

Storch et al ACS Synth Biol. 2015 Apr 16.

Insert

RBS34-mcherry-B0015

Expression

Bacterial

Available Since

Aug. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pBbS9a-RFP

Plasmid

#126172

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23113

Available Since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

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pLX-AS

Plasmid

#188870

Purpose

Compact T-DNA binary vector of the pLX series suitable for assembly of plant virus infectious clones and their Agrobacterium-mediated delivery to plants

Depositor

Fabio Pasin

Article

Pasin et al STAR Protoc. 2022 Dec 16;3(4):101716. do

Insert

none

Use

Synthetic Biology; Binary t-dna vector

Expression

Plant

Promoter

cauliflower mosaic virus (CaMV) 35S promoter

Available Since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

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Arp3-TagRFP

Plasmid

#195091

Purpose

Gene Overexpression of Human Arp3

Depositor

Jukka Westermarck

Article

Butt et al J Cell Sci. 2022 Oct 15;135(20):jcs26062

Insert

Arp3 (APOBEC3A Human)

Expression

Mammalian

Available Since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pGWB2-CYCD3;1

Plasmid

#140750

Purpose

Inducing plant cells to enter mitosis

Depositor

Bo Liu

Article

Xu et al New Phytol. 2019 Nov 2. doi: 10.1111/nph

Insert

Cyclin D3;1 (CYCD3;1 Mustard Weed)

Expression

Plant

Promoter

35S

Available Since

May 6, 2020

Availability

Academic Institutions and Nonprofits only

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pTRANS_211

Plasmid

#91110

Purpose

Transformation, Type: T-DNA, Plant Selection: 2x35S:hpt II, Viral Replication: BeYDV

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Expression

Plant

Promoter

35S

Available Since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pET28b-pchG WT

Plasmid

#87107

Purpose

expresses PchG WT, the thiazolinyl imine reductase from Pseudomonas aeruginosa

Depositor

Audrey Lamb

Article

Meneely et al Biochemistry. 2016 Sep 27;55(38):5423-33

Insert

pchG WT

Tags

his-tag

Expression

Bacterial

Promoter

T7

Available Since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-CaM-V5-sTb(N)

Plasmid

#219779

Purpose

sTb(N) half of CaST for HEK cell expression

Depositor

Christina Kim

Article

Zhang et al Nat Methods. 2024 Sep;21(9):1725-1735. d

Insert

CaM-V5-sTb(N)

Use

AAV

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pTmpGm

Plasmid

#176229

Purpose

A template plasmid for amplification of the cI-hok-aacC4 cassette

Depositor

Sergey Sineoky

Article

Bubnov et al Nucleic Acids Res. 2022 Aug 26;50(15):89

Insert

cI of phage lambda, hok of the R1 plasmid, aacC4

Use

Synthetic Biology

Expression

Bacterial

Available Since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pUCBB-oriT

Plasmid

#32556

Depositor

Claudia Schmidt-dannert

Article

Vick et al Appl Microbiol Biotechnol. 2011 Dec;92(6

Insert

oriT

Use

Synthetic Biology

Expression

Bacterial

Promoter

N/A

Available Since

Oct. 18, 2011

Availability

Academic Institutions and Nonprofits only

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pGST2-TSG101 (2-145)

Plasmid

#184810

Purpose

Bacterial expression of TSG101 UEV domain (aa 2-145)

Depositor

James Hurley

Article

Im et al Structure. 2010 Nov 10. 18(11):1536-47.

Insert

TSG101 (TSG101 Human)

Tags

GST

Expression

Bacterial

Mutation

aa 2-145 only

Promoter

tac

Available Since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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pET-6xHis-GFP(+18)

Plasmid

#199162

Purpose

used to express a supercharged GFP variant in E. coli

Depositor

Allie Obermeyer

Article

Yeong et al ACS Cent Sci. 2020 Dec 23;6(12):2301-231

Insert

GFP(+18)

Tags

MGHHHHHHGGA

Mutation

E16R, D29K, E42K, E44K, D86K, D112K, T115K, D127R…

Promoter

T7

Available Since

May 10, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-L26-Flag

Plasmid

#19972

Depositor

Moshe Oren

Article

Ofir-Rosenfeld et al Mol Cell. 2008 Oct 24. 32(2):180-9.

Insert

Rpl26 (RPL26 Human)

Tags

Flag

Expression

Mammalian

Available Since

Jan. 29, 2009

Availability

Academic Institutions and Nonprofits only
pET-6xHis-GFP(+12)

Plasmid

#199161

Purpose

used to express a supercharged GFP variant in E. coli

Depositor

Allie Obermeyer

Article

Yeong et al ACS Cent Sci. 2020 Dec 23;6(12):2301-231

Insert

GFP(+12)

Tags

MGHHHHHHGGA

Mutation

E16R, E44K, D86K, D112K, T115K, D127R, I138R, E15…

Promoter

T7

Available Since

May 10, 2023

Availability

Academic Institutions and Nonprofits only

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pGL2_163

Plasmid

#199095

Purpose

Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

pAMβ1 ORI plus NGS barcode 3, gentamicin resistance cassette, mScarlet-I cassette, oriT

Use

Synthetic Biology

Available Since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA-ZapCY1

Plasmid

#36319

Depositor

Amy Palmer

Article

Qin et al Proc Natl Acad Sci U S A. 2011 May 3;108

Insert

ZapCY1 high affinity Zn(II) sensor

Expression

Mammalian

Promoter

CMV

Available Since

May 18, 2012

Availability

Academic Institutions and Nonprofits only

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pSUPER.retro.puro-shNC

Plasmid

#113535

Purpose

Control retroviral vector.

Depositor

Blerta Xhemalce

Article

Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

Insert

shNC (scrambled control shRNA)

Use

Retroviral

Available Since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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pHCKan-yibDp-ald*-alaE

Plasmid

#134939

Purpose

Low phosphate inducible alanine dehydrogenase and alanine exporter

Depositor

Michael Lynch

Article

Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

Insert

alanine dehydrogenase and L-alanine exporter

Expression

Bacterial

Promoter

yibD

Available Since

May 5, 2020

Availability

Academic Institutions and Nonprofits only

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pAV0765

Plasmid

#133501

Purpose

Expression of mCherry targeted to the nucleus with one NLS signals

Depositor

Sophie Martin

Article

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pLys3(BstZ17I)-p(tdh1)-NLSSV40-mCherry-terminator(ScADH1)-kanMX

Expression

Yeast

Available Since

Nov. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pAV0532

Plasmid

#133502

Purpose

Expression of mTagBFP2 targeted to the nucleus with one NLS signals

Depositor

Sophie Martin

Article

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pLys3(BstZ17I)-p(tdh1)-NLSSV40-mTagBFP2-terminator(tdh1)-kanMX

Expression

Yeast

Available Since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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