We narrowed to 58,607 results for: plasmid
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Plasmid#113626PurposesgRNA/CAS9 expression plasmid to induce a 3’ double-strand break in the 4-μHOM reporter 8 nts. upstream from the microhomologyDepositorInsert7i sgRNA
UseCRISPRExpressionMammalianAvailable SinceAug. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYPQ166
Plasmid#109328PurposeGateway entry plasmid (attL1 & attR5) expressing 3_FLAG-NLS-zCas9-NLS without promoterDepositorInsertzCas9
UseCRISPR; Gateway compatible zcas9 entry cloneTags3x FLAG, NLS and NLSExpressionPlantMutationZea mays codon-optimized Cas9Available SinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
1a. pETM11-SUMO-SNCA-GFP
Plasmid#107292PurposePlasmid for the expression of the construct alpha-Synuclein-GFP, which contains N-terminal tags, which can be cleaved later: His-tag for purification and SUMO-tag for better solubilityDepositorAvailable SinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-FLAG-CXCR4
Plasmid#98947PurposeMammalian expression plasmid for N-terminal FLAG-tagged human CXCR4DepositorInsertCXCR4 (CXCR4 Human)
TagsFLAG (dykdddd) epitope tag and Signal/leader sequ…ExpressionMammalianMutationFull-length, wildtypePromoterCMVAvailable SinceApril 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
IFNAR1_pCSdest
Plasmid#53881DepositorAvailable SinceMarch 27, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCW57.1 _3xHA-Ago2
Plasmid#73538PurposeInducible lentiviral expression of Ago2DepositorInsertAgo2 (Ago2 Mouse)
UseLentiviralTags3xHAExpressionMammalianPromoterTRE promoter, Tet ONAvailable SinceFeb. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDB4282
Plasmid#98701PurposeA plasmid containing the 3' portion of the ura4 marker and the gRNA elements downstream of the target sequence. It serves as the PCR template for generating the gRNA insert in the split-ura4 systemDepositorInsertgRNA PCR template
UseCRISPRExpressionYeastAvailable SinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
Lv EF1a SS18-Frb PGK Puro
Plasmid#102811PurposeLentiviral plasmid from FIRE-Cas9 system to express SS18-Frb fusion proteinDepositorInsertSS18-Frb
UseCRISPR and LentiviralExpressionMammalianPromoterEF1aAvailable SinceDec. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTECH-MbAcK3RS(IPYE)
Plasmid#104070PurposeExpression plasmid encoding MbAcK3RS(IPYE) and pylTDepositorInsertPlpp MbAcK3RS(IPYE); PproK pyl
ExpressionBacterialMutationContains activating mutations V31I, T56P, H62Y, A…PromoterPlpp and PproKAvailable SinceDec. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
8024 GFP_Kras_G12C_2A_puro
Plasmid#64373PurposeThis a retroviral expression plasmid expressing GFP tagged G12C mutant Kras 2A oncogene along with puro resistance geneDepositorAvailable SinceJuly 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
7TFP CDH1 mutant reporter
Plasmid#91703Purposelentiviral luciferase reporter containing CDH1 promoter with mutant E-boxesDepositorInsertCDH1 mutant promoter (CDH1 Human)
UseLentiviral and LuciferaseTagsluciferaseExpressionMammalianMutationmutant E-boxesPromoterCDH1 mutantAvailable SinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pIMP1-Pthl
Plasmid#90485PurposePlasmid with thl(CA_C2873) promoter for gene expression in Clostridium acetobutylicum ATCC 824 and Clostridium beijerinckii NCIMB8052DepositorTypeEmpty backboneExpressionBacterialAvailable SinceMay 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTTP1A
Plasmid#91721Purposeintegration-proficient plasmid vector for mycobacteria, contains the attP and int region from Tweety genomic DNADepositorTypeEmpty backboneUseIntegration-proficient plasmid vectors for mycoba…Available SinceMay 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_FBXW7_WT
Plasmid#81973PurposeGateway Donor vector containing FBXW7, part of the Target Accelerator Plasmid Collection.DepositorAvailable SinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_TP53_WT
Plasmid#82754PurposeGateway Donor vector containing TP53, part of the Target Accelerator Plasmid Collection.DepositorAvailable SinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_SMAD5_WT
Plasmid#82217PurposeGateway Donor vector containing SMAD5 , part of the Target Accelerator Plasmid Collection.DepositorAvailable SinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_BRAF_p.V600E
Plasmid#82186PurposeGateway Donor vector containing BRAF , part of the Target Accelerator Plasmid Collection.DepositorAvailable SinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_SNAI2_WT
Plasmid#82162PurposeGateway Donor vector containing SNAI2 , part of the Target Accelerator Plasmid Collection.DepositorAvailable SinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAi14-GFPNLS-MC
Plasmid#87114Purposeminicircle parental backbone plasmid including GFPNLS-pA knock-in donor and one cutting site for HITIDepositorInsertGFPNLSPpA
UseCRISPRPromoternEFAvailable SinceMarch 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pY036_ATP1A1_G5_Array
Plasmid#86620PurposeVector for expression of a CRISPR/AsCpf1 array containing the ATP1A1 G5 guide in combination with a user-specified guide. Cloning of oligos for the second guide using BbsI sites. PY036-like plasmidDepositorInsertATP1A1 G5 crRNA + user-specified crRNA + AsCpf1-3xHA
UseCRISPR; Co-selection via hdr using ouabainTags3xHAExpressionMammalianPromoterCBhAvailable SinceMarch 16, 2017AvailabilityAcademic Institutions and Nonprofits only