We narrowed to 56,182 results for: plasmid

1. pK3F-GAL1

   Plasmid

   #85772

   Purpose

   N-ICE plasmid pK3F-GAL1

   Depositor

   Scott Briggs

   Article

   Zhang et al Yeast. 2016 Dec 12. doi: 10.1002/yea.322

   Insert

   loxP-KanMX-loxP-GAL1p-3FLAG
   

   Use

   Cre/Lox

   Expression

   Yeast

   Available Since

   Jan. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pH3FS-PYK1

   Plasmid

   #85781

   Purpose

   N-ICE plasmid pH3FS-PYK1

   Depositor

   Scott Briggs

   Article

   Zhang et al Yeast. 2016 Dec 12. doi: 10.1002/yea.322

   Insert

   loxP-HphMX-loxP-PYK1p-3FLAG
   

   Use

   Cre/Lox

   Expression

   Yeast

   Available Since

   May 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDOC-K-glmS-lacIZ

   Plasmid

   #158061

   Purpose

   Donor plasmid for insertion of the lacIZ operon and lac promoter into the S. Typhimurium chromosome

   Depositor

   Mark Webber

   Article

   Holden et al Microbiology (Reading). 2020 Nov 23. doi

   Insert

   lacIZ operon
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   lac

   Available Since

   Sept. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJ291_WT_med

   Plasmid

   #128856

   Purpose

   Medium copy number plasmid for expression of E. coli flagellin from an IPTG-inducible T5 promoter

   Depositor

   Mark Pallen

   Article

   Thomson et al Curr Res Biotechnol. 2020 Nov;2:45-52. d

   Insert

   Flagellin
   

   Expression

   Bacterial

   Available Since

   Feb. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGBW-m4046464

   Plasmid

   #148970

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
   

   Tags

   Cleavable thrombin;3xFLAG

   Expression

   Yeast

   Mutation

   wt

   Promoter

   pGAL1

   Available Since

   May 14, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pK3FS-GAL1

   Plasmid

   #85778

   Purpose

   N-ICE plasmid pK3FS-GAL1

   Depositor

   Scott Briggs

   Article

   Zhang et al Yeast. 2016 Dec 12. doi: 10.1002/yea.322

   Insert

   loxP-KanMX-loxP-GAL1p-3FLAG
   

   Use

   Cre/Lox

   Expression

   Yeast

   Available Since

   Jan. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTFE3-donor

   Plasmid

   #112377

   Purpose

   CRISPR donor plasmid to tag human transcription factor TFE3 with GFP

   Depositor

   Kevin White

   Insert

   TFE3 homology arms flanking EGFP-IRES-Neo cassette (TFE3 Human)
   

   Use

   CRISPR

   Mutation

   hg38:X:49030928:T>A,hg38:X:49030929:A>T,hg3…

   Available Since

   Dec. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGBW-m4046574

   Plasmid

   #148976

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
   

   Tags

   Cleavable thrombin;3xFLAG

   Expression

   Yeast

   Mutation

   Saccharomyces cerevisiae recode 1

   Promoter

   pGAL1

   Available Since

   May 14, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. CD44I-c001

   Plasmid

   #175448

   Purpose

   Protein expression in bacterial cells (as inclusion bodies). CD44 Link module, M63-N172.

   Depositor

   Opher Gileadi

   Insert

   CD44 (CD44 Human)
   

   Tags

   His6-TEV

   Expression

   Bacterial

   Available Since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pH3F

    Plasmid

    #85773

    Purpose

    N-ICE plasmid pH3F

    Depositor

    Scott Briggs

    Article

    Zhang et al Yeast. 2016 Dec 12. doi: 10.1002/yea.322

    Insert

    loxP-HphMX-loxP-3FLAG
    

    Use

    Cre/Lox

    Expression

    Yeast

    Available Since

    Jan. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. Col7A1-CRISPR-gRNA#exon3-(pSpCas9(BB)-2A-Puro (PX459) V2.0)

    Plasmid

    #124287

    Purpose

    sgRNA against human Col7A1

    Depositor

    Katalin Josvay, Csaba Vizler

    Insert

    Collagen type VII alpha 1 chain (COL7A1 Human)
    

    Use

    CRISPR

    Available Since

    April 3, 2019

    Availability

    Academic Institutions and Nonprofits only

12. pGBW-m4046286

    Plasmid

    #149147

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 surface glycoprotein (S Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    

    Tags

    Cleavable thrombin;3xFLAG

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pTEF1

    Available Since

    May 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pISlin41w(s) (lin-41 microRNA wt reporter)

    Plasmid

    #11906

    Depositor

    David Bartel

    Article

    Lewis et al Cell. 2003 Dec 26. 115(7):787-98.

    Insert

    lin-41 miRNA target sequence (lin-41 Nematode)
    

    Use

    Luciferase

    Expression

    Mammalian

    Available Since

    April 27, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJ291_WT_low

    Plasmid

    #128855

    Purpose

    Low copy number plasmid for expression of E. coli flagellin from an IPTG-inducible T5 promoter

    Depositor

    Mark Pallen

    Article

    Thomson et al Curr Res Biotechnol. 2020 Nov;2:45-52. d

    Insert

    Flagellin
    

    Expression

    Bacterial

    Available Since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. JARID1BA

    Plasmid

    #74648

    Purpose

    Baculovirus expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    JARID1BA (KDM5B Human)
    

    Use

    Baculovirus

    Tags

    His-6-TEV

    Mutation

    Leu101-(Gly)4-Ala374 deletion mutant

    Promoter

    PH

    Available Since

    April 21, 2016

    Availability

    Academic Institutions and Nonprofits only

16. p183_LTJ_sgRNACD90.2_A

    Plasmid

    #82671

    Purpose

    sgRNA targeting murine CD90.2. Co-expresses SpCas9-2A-EGFP.

    Depositor

    Lukas Jeker

    Article

    Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

    Insert

    sgRNA targeting CD90.2
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Jan. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMLX-donor

    Plasmid

    #112370

    Purpose

    CRISPR donor plasmid to tag human transcription factor MLX with GFP

    Depositor

    Kevin White

    Insert

    MLX homology arms flanking EGFP-IRES-Neo cassette (MLX Human)
    

    Use

    CRISPR

    Mutation

    hg38:17:42570798:G>A, hg38:17:42570950:T>G,…

    Available Since

    Dec. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGBW-m4164344

    Plasmid

    #153554

    Purpose

    Mammalian expression plasmid for SARS-CoV-2 S (surface glycoprotein)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    accatg Kozak-START linker

    Expression

    Mammalian

    Mutation

    Gordon et al recoded

    Available Since

    June 16, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pJ211_rha_WT_med

    Plasmid

    #128852

    Purpose

    Medium copy number plasmid for expression of E. coli flagellin from a rhamnose-inducible promoter

    Depositor

    Mark Pallen

    Article

    Thomson et al Curr Res Biotechnol. 2020 Nov;2:45-52. d

    Insert

    Flagellin
    

    Expression

    Bacterial

    Available Since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pK3F-PYK1

    Plasmid

    #85771

    Purpose

    N-ICE plasmid pK3F-PYK1

    Depositor

    Scott Briggs

    Article

    Zhang et al Yeast. 2016 Dec 12. doi: 10.1002/yea.322

    Insert

    loxP-KanMX-loxP-PYK1p-3FLAG
    

    Use

    Cre/Lox

    Expression

    Yeast

    Available Since

    Jan. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge