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GB2889

Plasmid

#193127

Purpose

A2 Proximal promoter sequence consisting of two times the target sequence for the gRNA1 (GB1838) flanked by random sequences.

Depositor

Diego Orzaez

Article

Moreno-Gimenez et al ACS Synth Biol. 2022 Sep 16;11(9):3037-3

Insert

GB_SynP (A2) G1ab.5

Use

Synthetic Biology

Available Since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

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GB3272

Plasmid

#193134

Purpose

A2 Proximal promoter sequence consisting of the target sequence for the gRNA1 (GB1838) at "b site" (-210 from TSS).

Depositor

Diego Orzaez

Article

Moreno-Gimenez et al ACS Synth Biol. 2022 Sep 16;11(9):3037-3

Insert

GB_SynP (A2) G1b.1

Use

Synthetic Biology

Available Since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

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GB2891

Plasmid

#193128

Purpose

A2 Proximal promoter sequence consisting of three times the target sequence for the gRNA1 (GB1838) flanked by random sequences.

Depositor

Diego Orzaez

Article

Moreno-Gimenez et al ACS Synth Biol. 2022 Sep 16;11(9):3037-3

Insert

GB_SynP (A2) G1abc.1

Use

Synthetic Biology

Available Since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

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GB2885

Plasmid

#193123

Purpose

A2 Proximal promoter sequence consisting of two times the target sequence for the gRNA1 (GB1838) flanked by random sequences.

Depositor

Diego Orzaez

Article

Moreno-Gimenez et al ACS Synth Biol. 2022 Sep 16;11(9):3037-3

Insert

GB_SynP (A2) G1ab.1

Use

Synthetic Biology

Available Since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

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GB2879

Plasmid

#193116

Purpose

A2 Proximal promoter sequence consisting of the target sequences for the gRNA1 (GB1838) and gRNA2 (GB1839) flanked by random sequences.

Depositor

Diego Orzaez

Article

Moreno-Gimenez et al ACS Synth Biol. 2022 Sep 16;11(9):3037-3

Insert

GB_SynP (A2) G1aG2b.5

Use

Synthetic Biology

Available Since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

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GB3278

Plasmid

#193132

Purpose

A2 Proximal promoter sequence consisting of three times the target sequence for the gRNA1 (GB1838) flanked by random sequences.

Depositor

Diego Orzaez

Article

Moreno-Gimenez et al ACS Synth Biol. 2022 Sep 16;11(9):3037-3

Insert

GB_SynP (A2) G1abc.5

Use

Synthetic Biology

Available Since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

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GB3273

Plasmid

#193135

Purpose

A2 Proximal promoter sequence consisting of the target sequence for the gRNA1 (GB1838) at "e site" (-320 from TSS).

Depositor

Diego Orzaez

Article

Moreno-Gimenez et al ACS Synth Biol. 2022 Sep 16;11(9):3037-3

Insert

GB_SynP (A2) G1e.1

Use

Synthetic Biology

Available Since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pLY227

Plasmid

#184148

Purpose

short crRNA generator with spacer LEA2 with WT repeat region

Depositor

Baojun Wang

Article

Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

Insert

s-crRNA-LEA2-WT

Use

Synthetic Biology

Promoter

Plux2

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pnYC-NpM-HsSMG7opt_732-1091_V

Plasmid

#147769

Purpose

Bacterial Expression of HsSMG7opt_732-1091

Depositor

Elisa Izaurralde

Article

Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

Insert

HsSMG7opt_732-1091 (SMG7 Human)

Expression

Bacterial

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pLY171

Plasmid

#184145

Purpose

sgRNA with 3 RNA aptemers BoxB at the 3'-end for CRISPRa

Depositor

Baojun Wang

Article

Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

Insert

sgRNA-LEA2-ex3

Use

Synthetic Biology

Promoter

Plux2

Available Since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pRB121

Plasmid

#181945

Purpose

aTc-inducible expression of dimerized mNeonGreen (mNG-mNG)

Depositor

Jeffrey Tabor

Article

Butcher et al Proc Natl Acad Sci U S A. 2022 Aug 30;11

Insert

mNeonGreen-mNeonGreen; tetR (AX04_RS23485 Synthetic)

Expression

Bacterial

Mutation

See Depositor Comments Below

Promoter

mNG-mNG-PLtetO-1; tetR-J23106

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST-pSP172BSSPE-R3-ccdB/CmR-R5::RfA

Plasmid

#186407

Purpose

Multisite destination vector for ascidian electroporation of an AttB1/B2 recombined gene of interest under control of an AttB3/B5 recombined reg. sequence.

Depositor

Patrick Lemaire

Article

Roure et al PLoS One. 2007. 2(9):e916.

Type

Empty backbone

Use

Gateway destination vector

Available Since

Oct. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST-pSP172BSSPE-pFOGc::RfA

Plasmid

#186397

Purpose

Destination vector for ascidian transgenesis by electroporation of a Gateway-cloned gene of interest under control of the pFOG ectodermal regulatory sequence.

Depositor

Patrick Lemaire

Article

Roure et al PLoS One. 2007. 2(9):e916.

Type

Empty backbone

Use

Gateway destination vector

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST-pSP172BSSPE-Swa1::RfA

Plasmid

#186396

Purpose

Destination vector for ascidian transgenesis by electroporation of a Gateway-cloned gene of interest under control of a regulatory sequence cloned in the Swa1 restriction site.

Depositor

Patrick Lemaire

Article

Roure et al PLoS One. 2007. 2(9):e916.

Type

Empty backbone

Use

Gateway destination vector

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST tol2 UbB polyA

Plasmid

#188701

Purpose

Gateway destination vector containing the zebrafish ubiquitin B polyadenylation sequence

Depositor

David Tobin

Article

Walton et al PLoS One. 2015 Oct 7;10(10):e0138949. do

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Available Since

Aug. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pGP4G-REL2N91-ccdB

Plasmid

#188444

Purpose

Zea Mays Nuclear Auxin Signaling CoRepressor REL2N91 and pDEST

Depositor

Britney Moss

Article

Ramos Baez et al Plant Physiol. 2020 Apr;182(4):1713-1722

Insert

REL2N91/ccdB

Expression

Yeast

Available Since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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PDEST-pSP172BSSPE-pFOGc::RfA-HA

Plasmid

#186406

Purpose

Destination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of a gene of interest with a C-ter HA tag under control of the pFOG ectodermal regulatory sequence.

Depositor

Patrick Lemaire

Article

Roure et al PLoS One. 2007. 2(9):e916.

Type

Empty backbone

Use

Gateway destination vector

Available Since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pEPQD0CM0033

Plasmid

#185624

Purpose

Level 0 promoter from N. benthamiana used for sgRNA expression, nonstandard overlaps, GGAG-CTCG

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

NbU6-2

Use

CRISPR and Synthetic Biology

Available Since

Aug. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pEPQD0CM0032

Plasmid

#185623

Purpose

Level 0 promoter from N. benthamiana used for sgRNA expression, nonstandard overlaps, GGAG-TTCG

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

NbU6-1

Use

CRISPR and Synthetic Biology

Available Since

Aug. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pCJH026_proD_Cas12c-multiplexing_CAM

Plasmid

#183073

Purpose

Plasmid expressing a Cas12c sgRNA targeting GFP and another sgRNA targeting RFP in one transcript for testing multiplexed repression

Depositor

Jennifer Doudna

Article

Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

Insert

Expressing two Cas12c sgRNAs (targeting GFP or RFP) in one transcript

Use

CRISPR

Expression

Bacterial

Available Since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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