We narrowed to 11,552 results for: ANG

Showing: 8101 - 8120 of 11552 results

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LENTICRISPR-LUC7L3_sgRNA1

Plasmid

#201604

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

Insert

LUC7L3 (LUC7L3 Human)

Use

CRISPR and Lentiviral

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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LENTICRISPR-LUC7L3_sgRNA2

Plasmid

#201605

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

Insert

LUC7L3 (LUC7L3 Human)

Use

CRISPR and Lentiviral

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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LENTICRISPR-NDUFB5_sgRNA1

Plasmid

#201606

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

Insert

NDUFB5 (NDUFB5 Human)

Use

CRISPR and Lentiviral

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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LENTICRISPR-NDUFB5_sgRNA2

Plasmid

#201607

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

Insert

NDUFB5 (NDUFB5 Human)

Use

CRISPR and Lentiviral

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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lenti-Cas9-sgHPRT1

Plasmid

#196713

Purpose

CRISPR-KO. WT-SpCas9 and sgRNA targeting HPRT1. Editing-competent cells can be selected with 6-TG

Depositor

Bernhard Schmierer

Article

Schmierer et al Mol Syst Biol. 2017 Oct 9;13(10):945. do

Insert

Cas9-T2A-BSD-U6-sgHPRT1

Use

CRISPR and Lentiviral

Tags

Nucleoplasmin NLS and SV40 NLS

Promoter

EF1a/hU6

Available Since

April 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-RLuc-BGG-PTC_E

Plasmid

#146191

Purpose

Mammalian Expression of BGG-PTC

Depositor

Elisa Izaurralde

Article

Jonas et al Genes Dev. 2013 Jan 15;27(2):211-25. doi

Insert

BGG-PTC (HBB Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-RLuc-BGG-WT_E

Plasmid

#146192

Purpose

Mammalian Expression of BGG-WT

Depositor

Elisa Izaurralde

Article

Jonas et al Genes Dev. 2013 Jan 15;27(2):211-25. doi

Insert

BGG-WT (HBB Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmeEF1A_R

Plasmid

#147347

Purpose

Insect Expression of DmeIF1A

Depositor

Elisa Izaurralde

Insert

DmeIF1A (eIF-1A Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmeEF1A_R

Plasmid

#147349

Purpose

Insect Expression of DmeIF1A

Depositor

Elisa Izaurralde

Insert

DmeIF1A (eIF-1A Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpG-DmNot1_1499-1644_R

Plasmid

#147355

Purpose

Bacterial Expression of DmNot1_1499-1644

Depositor

Elisa Izaurralde

Insert

DmNot1_1499-1644 (Not1 Fly)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-DmNot1_1499-1644_R

Plasmid

#147357

Purpose

Bacterial Expression of DmNot1_1499-1644

Depositor

Elisa Izaurralde

Insert

DmNot1_1499-1644 (Not1 Fly)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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EGFP-QQN

Plasmid

#193563

Purpose

Expresses human Clathrin Light Chain B mutant (residues 20-22 were substituted from EED to QQN) tagged with mEGFP in mammalian cells.

Depositor

Justin Taraska

Article

Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

Insert

Clathrin light chain B (CLTB Human)

Tags

mEGFP

Expression

Mammalian

Mutation

Changed from EED to QQN at residue 20-22

Promoter

CMV

Available Since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NvM-DmNot1_1147-1717_P

Plasmid

#147187

Purpose

Bacterial Expression of DmNot1_1147-1717

Depositor

Elisa Izaurralde

Insert

DmNot1_1147-1717 (Not1 Fly)

Expression

Bacterial

Available Since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NvG-DmNot1_1147-1717_N

Plasmid

#147018

Purpose

Bacterial Expression of DmNot1_1147-1717

Depositor

Elisa Izaurralde

Insert

DmNot1_1147-1717 (Not1 Fly)

Expression

Bacterial

Available Since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmeIF4A_G

Plasmid

#146361

Purpose

Insect Expression of DmeIF4A

Depositor

Elisa Izaurralde

Insert

DmeIF4A (eIF-1A Fly)

Expression

Insect

Available Since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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eSpCas9-EF-5Flnc

Plasmid

#174870

Purpose

CRISPR vector for generating Flnc STREAMING-tag KI cell

Depositor

Hiroshi Ochiai

Article

Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

Insert

sgRNA for mouse Flnc (Flnc Synthetic)

Use

CRISPR

Available Since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

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FnRPR9-10

Plasmid

#182568

Purpose

Human fibronectin fragment, type-III repeats 9-10, R1374A, P1376A, R1379A mutant

Depositor

Timothy Springer

Article

Su et al J Biol Chem. 2022 Sep;298(9):102323. doi

Insert

Human fibronectin fragment, type-III repeats 9-10

Tags

His and TEV cleavage site after His tag

Expression

Bacterial

Mutation

R1374A, P1376A, R1379A

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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FnRPR8-10

Plasmid

#182570

Purpose

Human fibronectin fragment, type-III repeats 8-10, R1374A, P1376A, R1379A mutant

Depositor

Timothy Springer

Article

Su et al J Biol Chem. 2022 Sep;298(9):102323. doi

Insert

Human fibronectin fragment, type-III repeats 8-10

Tags

His and TEV cleavage site after His tag

Expression

Bacterial

Mutation

R1374A, P1376A, R1379A

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pJCC_103 SpCas9 T1337C

Plasmid

#179525

Purpose

For bacterial expression of SpCas9 T1337C (for DNA cross-linking) with an N-terminal His-MBP tag

Depositor

Jennifer Doudna

Article

Cofsky et al Nat Struct Mol Biol. 2022 Apr;29(4):395-

Insert

SpCas9 T1337C

Tags

10X His, TEV protease cleavage site, and maltose-…

Expression

Bacterial

Mutation

changed threonine 1337 to cysteine

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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pX459-sg-CMV152

Plasmid

#179916

Purpose

All in one Cas9 plasmid with puromycin resistance and a single guide RNA targeting cytomegaloviral promoter site 152.

Depositor

Indeever Madireddy, Johan Sosa

Article

Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

Insert

CMV promoter sgRNA

Use

CRISPR

Tags

2A-Puro and 3XFLAG

Expression

Mammalian

Promoter

U6

Available Since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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