We narrowed to 53,707 results for: Ide

1. pLKO.1B CDC25C_1

   Plasmid

   #160769

   Purpose

   Suppress CDC25C

   Depositor

   Richard Possemato

   Article

   Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

   Insert

   shCDC25C_1
   

   Use

   Lentiviral

   Available Since

   Oct. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLKO.1B CDC25A_1

   Plasmid

   #160767

   Purpose

   Suppress CDC25A

   Depositor

   Richard Possemato

   Article

   Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

   Insert

   shCDC25A_1
   

   Use

   Lentiviral

   Available Since

   Oct. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMTTH+LANA-H1.4CTD

   Plasmid

   #157798

   Purpose

   Expression of LANA peptide-human linker histone H1.4 CTD fusion protein

   Depositor

   Michael Rosen

   Article

   Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

   Insert

   LANA-H1.4CTD (H1-4 Human)
   

   Tags

   His6 and MBP

   Expression

   Bacterial

   Promoter

   pTAC

   Available Since

   Aug. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. INCTbiosyn-pUB-HspINT4

   Plasmid

   #127513

   Purpose

   Plasmid encodes H. sapiens codon optimized Integrase 4.

   Depositor

   Martin Bonamino

   Article

   Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

   Insert

   Integrase 4 coding sequence codon optimized for H. sapiens expression.
   

   Expression

   Mammalian

   Available Since

   May 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. INCTbiosyn-pUB-HspINT5

   Plasmid

   #127514

   Purpose

   Plasmid encodes H. sapiens codon optimized Integrase 5.

   Depositor

   Martin Bonamino

   Article

   Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

   Insert

   Integrase 5 coding sequence codon optimized for H. sapiens expression.
   

   Expression

   Mammalian

   Available Since

   May 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX330-Flag-evoSpCas9 (without sgRNA; with silent mutations)

   Plasmid

   #126758

   Purpose

   Expression plasmid for human codon-optimized increased fidelity evoSpCas9 (without U6-sgRNA coding sequence, with silent mutations)

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

   Insert

   evoSpCas9
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   M495V, Y515N, K526E, R661Q

   Promoter

   CBh

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX330-Flag-HeFSpCas9 (without sgRNA; with silent mutations)

   Plasmid

   #126759

   Purpose

   Expression plasmid for human codon-optimized increased fidelity HeFSpCas9 (without U6-sgRNA coding sequence, with silent mutations)

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

   Insert

   HeFSpCas9
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   N497A, R661A, Q695A, K848A, Q926A, K1003A, R1060A

   Promoter

   CBh

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pX330-Flag-eSpCas9 (without sgRNA; with silent mutations)

   Plasmid

   #126754

   Purpose

   Expression plasmid for human codon-optimized increased fidelity eSpCas9 (without U6-sgRNA coding sequence, with silent mutations)

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

   Insert

   eSpCas9
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   K848A, K1003A, R1060A

   Promoter

   CBh

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pX330-Flag-HypaSpCas9 (without sgRNA; with silent mutations)

   Plasmid

   #126756

   Purpose

   Expression plasmid for human codon-optimized increased fidelity HypaSpCas9 (without U6-sgRNA coding sequence, with silent mutations)

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

   Insert

   HypaSpCas9
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   N692A, M694A, Q695A, H698A

   Promoter

   CBh

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBADHISTEV_PfeEH258A

    Plasmid

    #129122

    Purpose

    expresses H258A mutant of PfeE in E.coli

    Depositor

    James Naismith

    Article

    Perraud et al ACS Chem Biol. 2018 Sep 21;13(9):2603-26

    Insert

    Periplasmic Esterase PfeE (PA2689 Pseudomonas aeruginosa PAO1)
    

    Tags

    TEV protease cleavable 6xHis

    Expression

    Bacterial

    Mutation

    Signal peptide sequence has been removed, mutat…

    Promoter

    PBAD

    Available Since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTAMAHISTEV_Ab-PirA

    Plasmid

    #128959

    Purpose

    expresses Acinetobacter baumannii PirA in E. coli

    Depositor

    James Naismith

    Article

    Moynie et al Antimicrob Agents Chemother. 2017 Mar 24

    Insert

    ferric enterobactin receptor PirA
    

    Tags

    TEV protease cleavable 7xHis and TamA

    Expression

    Bacterial

    Mutation

    Signal peptide sequence has been removed

    Promoter

    T7

    Available Since

    Aug. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBADHISTEV_PfeES157A

    Plasmid

    #129121

    Purpose

    expresses S157A mutant of PfeE in E.coli

    Depositor

    James Naismith

    Article

    Perraud et al ACS Chem Biol. 2018 Sep 21;13(9):2603-26

    Insert

    Periplasmic Esterase PfeE (PA2689 Pseudomonas aeruginosa PAO1)
    

    Tags

    TEV protease cleavable 6xHis

    Expression

    Bacterial

    Mutation

    Signal peptide sequence has been removed, mutat…

    Promoter

    PBAD

    Available Since

    Aug. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. mutpRL-TK 1x perfect to bulged siRNA

    Plasmid

    #40765

    Purpose

    To evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNA

    Depositor

    Phillip Zamore

    Article

    Broderick et al RNA. 2011 Oct;17(10):1858-69. Epub 2011

    Insert

    CXCR4 small RNA perfect to bulged target site
    

    Use

    Luciferase

    Tags

    luciferase

    Mutation

    mutated from TAG to CTC at nt 387–389 of the Reni…

    Promoter

    TK

    Available Since

    Dec. 18, 2013

    Availability

    Academic Institutions and Nonprofits only

14. mutpRL-TK 1x perfect to seed siRNA

    Plasmid

    #40766

    Purpose

    To evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNA

    Depositor

    Phillip Zamore

    Article

    Broderick et al RNA. 2011 Oct;17(10):1858-69. Epub 2011

    Insert

    CXCR4 small RNA perfect to seed target site
    

    Use

    Luciferase

    Tags

    luciferase

    Mutation

    mutated from TAG to CTC at nt 387–389 of the Reni…

    Promoter

    TK

    Available Since

    Sept. 26, 2013

    Availability

    Academic Institutions and Nonprofits only

15. mutpRL-TK 1x perfect to seed plus 13-16 siRNA

    Plasmid

    #40767

    Purpose

    To evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNA

    Depositor

    Phillip Zamore

    Article

    Broderick et al RNA. 2011 Oct;17(10):1858-69. Epub 2011

    Insert

    CXCR4 small RNA perfect to seed plus 13-16 target site
    

    Use

    Luciferase

    Tags

    luciferase

    Mutation

    mutated from TAG to CTC at nt 387–389 of the Reni…

    Promoter

    TK

    Available Since

    Sept. 26, 2013

    Availability

    Academic Institutions and Nonprofits only

16. 23SrRNA.RAV12(wt).2508-2580.GAAAC.duplexH90

    Plasmid

    #26192

    Depositor

    David McKay

    Article

    Hardin et al J Mol Biol. 2010 Sep 17;402(2):412-27. d

    Insert

    23S ribosomal RNA fragment (modified) with duplex H90
    

    Expression

    Bacterial

    Mutation

    fragment 2508-2580 of E. coli rRNA, with nucleoti…

    Available Since

    Dec. 8, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBS/U6 moesin siRNA

    Plasmid

    #8946

    Depositor

    Philip Hinds

    Article

    Yang et al Mol Cell. 2003 May . 11(5):1163-76.

    Insert

    moesin siRNA (MSN Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 6, 2005

    Availability

    Academic Institutions and Nonprofits only

18. Active Wnt8B-V5

    Plasmid

    #43819

    Depositor

    Xi He

    Article

    MacDonald et al J Biol Chem. 2014 May 19. pii: jbc.M114.

    Insert

    WNT8B (WNT8B Human)
    

    Tags

    V5

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. GST-MNase

    Plasmid

    #136291

    Purpose

    Expresses Micrococcal nuclease

    Depositor

    Kavitha Sarma

    Article

    Yan et al Cell Rep. 2019 Oct 29;29(5):1369-1380.e5

    Insert

    MNase
    

    Expression

    Bacterial

    Available Since

    Feb. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCS2-TAG

    Plasmid

    #26772

    Depositor

    Shankar Srinivas

    Article

    Trichas et al BMC Biol. 2008 Sep 15;6:40. doi: 10.1186

    Insert

    TdTomato-2A-H2BGFP-SV40pA
    

    Expression

    Mammalian

    Available Since

    March 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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