We narrowed to 15,991 results for: LEA

1. pCMV-T7-TadCBEd-SpG-P2A-EGFP (BKS777/AHK571)

   Plasmid

   #223124

   Purpose

   pCMV and pT7 Human expression plasmid for TadCBEd-SpG with C-term BPNLS-P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

   Insert

   human codon optimized TadCBEd-SpG-2xUGI-P2A-EGFP
   

   Use

   CRISPR

   Tags

   2xUGI-BPNLS-P2A-EGFP and BPNLS-TadA-CDd

   Expression

   Mammalian

   Mutation

   TadA-CDd mutations; nSpG=D10A/D1135L/S1136W/G1218…

   Promoter

   CMV and T7

   Available Since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcrDNA3.1-Chimera

   Plasmid

   #100891

   Purpose

   Mammalian expression of DNAJB1(exon1)-PRKACA(exon2-Cterminus) chimeric protein from fibrolamellar hepatocellular carcinoma

   Depositor

   Sanford Simon

   Article

   Honeyman et al Science. 2014 Feb 28;343(6174):1010-4. d

   Insert

   Major Fibrolamellar hepatocellular carcinoma Chimera (DNAJB1, PRKACA Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. TRIPZ-CTID195-GSQ-SUMO1nc-PURO

   Plasmid

   #208035

   Purpose

   Enables inducible expression of C-terminal Split-TurboID-fused SUMO1nc, to perform SUMO-ID; nc = non-cleavable mutation; selection with puromycin

   Depositor

   James Sutherland, Rosa Barrio

   Article

   Barroso-Gomila et al Nat Commun. 2021 Nov 18;12(1):6671. doi:

   Insert

   SUMO1nc (SUMO1 Human)
   

   Use

   Lentiviral

   Tags

   Myc, C-terminal Split-TurboID

   Mutation

   Q94P Mutation near C-terminus suppresses cleavage…

   Promoter

   tetO/UBC

   Available Since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-T7-ABE8e-SpCas9(MKRCMV)-P2A-EGFP (BKS1030)

   Plasmid

   #223115

   Purpose

   pCMV and pT7 Human expression plasmid for ABE8e using SpCas9 enzyme with MKRCMV amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

   Insert

   human codon optimized TadA8e-SpCas9(MKRCMV)-P2A-EGFP
   

   Use

   CRISPR

   Tags

   BPNLS-P2A-EGFP and BPNLS-TadA8e

   Expression

   Mammalian

   Mutation

   TadA8e mutations; nSpCas9(D10A/MKRCMV)=D1135M, S1…

   Promoter

   CMV and T7

   Available Since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-T7-ABE8e-SpCas9(MKRCKS)-P2A-EGFP (RAS3455)

   Plasmid

   #223116

   Purpose

   pCMV and pT7 Human expression plasmid for ABE8e using SpCas9 enzyme with MKRCKS amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

   Insert

   human codon optimized TadA8e-SpCas9(MKRCKS)-P2A-EGFP
   

   Use

   CRISPR

   Tags

   BPNLS-P2A-EGFP and BPNLS-TadA8e

   Expression

   Mammalian

   Mutation

   TadA8e mutations; nSpCas9(D10A/MKRCKS)=D1135M, S1…

   Promoter

   CMV and T7

   Available Since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHis6-SUMO-g0s2

   Plasmid

   #179293

   Purpose

   E. coli expression plasmid (T7 promoter) for full-length human G0S2 with N-terminal His6 + SUMO + TEV protease cleavage site

   Depositor

   Debra Hansen

   Article

   Moran et al PLoS One. 2021 Jul 14;16(7):e0249164. do

   Insert

   G0S2 (G0S2 Human)
   

   Tags

   His6 + SUMO + TEV protease cleavage site

   Expression

   Bacterial

   Mutation

   full-length G0S2, residues 1-103 from accession n…

   Promoter

   T7

   Available Since

   Feb. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pNTK Gatad2a flox

   Plasmid

   #110812

   Purpose

   Donor targeting vector for generating mouse Gatad2a conditional knockout allele

   Depositor

   Jacob Hanna

   Article

   Mor et al Cell Stem Cell. 2018 Sep 6;23(3):412-425

   Insert

   mGataD2a (Gatad2a Mouse)
   

   Use

   Unspecified

   Available Since

   Dec. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLIB_3xFlag-His-nsp5CS-nsp9 (SARS-CoV-2)

   Plasmid

   #169177

   Purpose

   Baculoviral transfer vector to express SARS-CoV-2 nsp9 with nsp5-cleavable 3xFlag-His6 tag in insect cells

   Depositor

   John Diffley

   Article

   Milligan et al Biochem J. 2021 Jul 16;478(13):2499-2515

   Insert

   3xFlag-His-nsp5CS-nsp9 (ORF1ab SARS-CoV-2)
   

   Tags

   3xFlag-His6 (nsp5-cleavable)

   Expression

   Insect

   Mutation

   Codon optimised for insect cells (Spodoptera frug…

   Promoter

   Polyhedrin

   Available Since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-T7-nSpRY(D10A)-BPNLS(SV40)-3xFLAG-P2A-EGFP (HES636)

   Plasmid

   #185491

   Purpose

   CMV and T7 promoter expression plasmid for human codon usage nSpRY(D10A) with a C-terminal bi-partite NLS, 3x FLAG, and P2A-eGFP

   Depositor

   Benjamin Kleinstiver, Raul Perez-Jimenez

   Article

   Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

   Insert

   human codon usage nSpRY(D10A)-BPNLS-P2A-eGFP
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS-3xFLAG-P2A-eGFP

   Expression

   Mammalian

   Mutation

   D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…

   Promoter

   CMV and T7

   Available Since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. PX459-gR2A_Hinge

    Plasmid

    #124811

    Purpose

    Vector for expression Cas9 with gRNA specific for rat IgG2a heavy chain locus. Use in combination with pHybr_r2a>Fab-srt-his plasmids to convert expression of hybridomas to Fab' fragments.

    Depositor

    Martijn Verdoes, Ferenc Scheeren

    Article

    van der Schoot et al Sci Adv. 2019 Aug 28;5(8):eaaw1822. doi:

    Insert

    Cas9 – gRNA_R2A_hinge
    

    Use

    CRISPR

    Tags

    3XFLAG and GFP

    Expression

    Bacterial and Mammalian

    Mutation

    R166H in PuroR (please see depositors comment bel…

    Promoter

    pUC

    Available Since

    Sept. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLY70

    Plasmid

    #130948

    Purpose

    A CRISPR activation device with the necessary genes (dxcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter PrhaB), and the reporter part (PpspA-LEA3B3 with sfgfp::ASV).

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

    Inserts

    dxcas9 (3.7)
    tetR
    rhaS
    pspFΔHTH::λN22plus
    sfgfp
    

    Use

    Synthetic Biology

    Tags

    ASV tag and pspFΔHTH::λN22plus

    Expression

    Bacterial

    Mutation

    Mutations D10A and H840A on WT cas9 for dCas9; Sy…

    Promoter

    Pcon, PpspA-LEA3B3, PrhaB, and Ptet

    Available Since

    Sept. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGL4.23-CD69 CaRE3 (plus strand)

    Plasmid

    #91844

    Purpose

    Luciferase reporter for CD69 enhancer (IGI-P0621)

    Depositor

    Jacob Corn

    Article

    Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

    Insert

    CD69 CaRE3 (plus strand) (CD69 Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    Missing 1 base from 13 base polyT tract beginning…

    Available Since

    May 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHIS-MDH1-HIS

    Plasmid

    #184558

    Purpose

    Bacterial expression of human MDH1 with HIS Tag

    Depositor

    Gerardo Ferbeyre

    Article

    Igelmann et al Mol Cell. 2021 Sep 16;81(18):3848-3865.e

    Insert

    Malate dehydrogenase 1 (MDH1 Human)
    

    Tags

    6xHIS

    Expression

    Bacterial

    Available Since

    June 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-T7-NLS(SV40)-anCas(BCA)-BPNLS(SV40)-P2A-EGFP (LTH1384)

    Plasmid

    #185488

    Purpose

    CMV and T7 promoter expression plasmid for human codon usage anCas(BCA) with an N-terminal NLS, C-terminal bi-partite NLS, and P2A-eGFP

    Depositor

    Benjamin Kleinstiver, Raul Perez-Jimenez

    Article

    Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

    Insert

    human codon usage NLS-anCas(BCA)-BPNLS-P2A-eGFP
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS-P2A-eGFP and NLS

    Expression

    Mammalian

    Promoter

    CMV and T7

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. MBP-g0s2

    Plasmid

    #179294

    Purpose

    E. coli expression plasmid (Ptac promoter) for full-length human G0S2 with N-terminal mature maltose binding protein + enterokinase cleavage site

    Depositor

    Debra Hansen

    Article

    Moran et al PLoS One. 2021 Jul 14;16(7):e0249164. do

    Insert

    G0S2 (G0S2 Human)
    

    Tags

    mature maltose binding protein + enterokinase cle…

    Expression

    Bacterial

    Mutation

    full-length G0S2, residues 1-103 from accession n…

    Promoter

    Ptac

    Available Since

    Feb. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. KRAS-A146T

    Plasmid

    #154426

    Purpose

    A146T Kras with Flag tag inserted into N-terminal domain between 6Xhis tag and TEV cleavage site.

    Depositor

    Kenneth Westover

    Article

    Poulin et al Cancer Discov. 2019 Jun;9(6):738-755. do

    Insert

    KRAS (KRAS Human)
    

    Tags

    6xHis-tag and TEV protease cleavage sequence

    Expression

    Bacterial

    Promoter

    T5

    Available Since

    Sept. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-T7-NLS(SV40)-anCas(PDCA)-BPNLS(SV40)-P2A-EGFP (LTH1430)

    Plasmid

    #185485

    Purpose

    CMV and T7 promoter expression plasmid for human codon usage anCas(PDCA) with an N-terminal NLS, C-terminal bi-partite NLS, and P2A-eGFP

    Depositor

    Benjamin Kleinstiver, Raul Perez-Jimenez

    Article

    Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

    Insert

    human codon usage NLS-anCas(PDCA)-BPNLS-P2A-eGFP
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS-P2A-eGFP and NLS

    Expression

    Mammalian

    Promoter

    CMV and T7

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-T7-nSpCas9(H840A)-BPNLS(SV40)-3xFLAG-P2A-EGFP (HES140)

    Plasmid

    #185490

    Purpose

    CMV and T7 promoter expression plasmid for human codon usage nSpCas9(H840A) with a C-terminal bi-partite NLS, 3x FLAG, and P2A-eGFP

    Depositor

    Benjamin Kleinstiver, Raul Perez-Jimenez

    Article

    Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

    Insert

    human codon usage nSpCas9(H840A)-BPNLS-P2A-eGFP
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-eGFP

    Expression

    Mammalian

    Mutation

    H840A

    Promoter

    CMV and T7

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLY61

    Plasmid

    #130928

    Purpose

    A CRISPR activation device with the necessary genes (dxcas9 3.7 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-LEA1B1 with sfgfp::ASV).

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

    Inserts

    dxcas9 (3.7)
    tetR
    pspFΔHTH::λN22plus
    sfgfp
    

    Use

    Synthetic Biology

    Tags

    ASV tag and pspFΔHTH::λN22plus

    Expression

    Bacterial

    Mutation

    Mutations D10A and H840A on WT cas9 for dCas9; Sy…

    Promoter

    Anderson promoter: J23106, PpspA-LEA1B1, and Ptet

    Available Since

    Sept. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. t206-405

    Plasmid

    #166132

    Purpose

    For expression of human talin-1 head fragment (residues 206-405) in E. coli. N-terminal His6-tag, Xpress-epitope (DLYDDDDK) and enterokinase cleavage site for tag removal.

    Depositor

    Vesa Hytönen

    Article

    Kukkurainen et al J Cell Sci. 2020 Oct 12;133(19). pii: 13

    Insert

    T1head1-F2F3(206-405) (TLN1 Human)
    

    Tags

    His6-tag, Xpress-epitope (DLYDDDDK) and enterokin…

    Expression

    Bacterial

    Mutation

    residues 206-405 only

    Available Since

    April 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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