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pAAV_CAG_Kinprola_PKA-mEGFP-NLS3__WPRE-SV40

Plasmid

#233365

Purpose

CAG driven expression of the PKA activity recorder Kinprola_PKA fused to mEGFP for mammalian cell expression through AAV transduction

Depositor

Kai Johnsson

Article

Sun et al Nat Chem Biol. 2025 Jul 10. doi: 10.1038

Insert

Kinprola_PKA-mEGFP-NLS3X

Use

AAV

Tags

mEGFP-NLS3X

Promoter

CAG

Available Since

April 14, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV_CAG_NES-Kinprola_PKA-mTagBFP2_WPRE-SV40

Plasmid

#233364

Purpose

CAG driven expression of the PKA activity recorder Kinprola_PKA fused to mTagBFP2 for mammalian cell expression through AAV transduction

Depositor

Kai Johnsson

Article

Sun et al Nat Chem Biol. 2025 Jul 10. doi: 10.1038

Insert

NES-Kinprola_PKA-mTagBFP2

Use

AAV

Tags

NES and mTagBFP2

Promoter

CAG

Available Since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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pXG290

Plasmid

#141284

Purpose

OE of HRI

Depositor

Martin Kampmann

Article

Guo et al Nature. 2020 Mar;579(7799):427-432. doi:

Insert

HRI (EIF2AK1 Human)

Tags

mClover

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-haA3A-CBE-A-NG

Plasmid

#220895

Purpose

Express haA3A-CBE-A-NG (with SpCas9-NG variant) in mammalian cells

Depositor

Dali Li

Article

Yang et al Nat Chem Biol. 2024 Mar 29. doi: 10.1038

Insert

haA3A-CBE-A-NG

Expression

Mammalian

Available Since

July 26, 2024

Availability

Academic Institutions and Nonprofits only

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pX601-AAV-CMV-NLS-ISTfu1-NLS-3xHA-bGHpA;U6-BbsI-reRNA

Plasmid

#205319

Purpose

All-in-one AAV vector expressing ISTfu1 TnpB and its reRNA

Depositor

Haoyi Wang

Article

Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

Insert

humanized ISTfu1 TnpB

Use

AAV

Tags

3 x HA

Expression

Mammalian

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HaloAKAR2.1-CLC

Plasmid

#216492

Purpose

Expression of chemigenetic PKA biosensor (medium basal brightness, high dynamic range) targeted to clathrin coated pits

Depositor

Jin Zhang

Article

Frei et al Nat Biotechnol. 2025 Apr 21. doi: 10.103

Insert

HaloAKAR2.1-CLC

Expression

Mammalian

Promoter

CMV

Available Since

June 20, 2025

Availability

Academic Institutions and Nonprofits only

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pSL0375 (His-MBP-TniQ_Cas8_Cas7_Cas6)

Plasmid

#130640

Purpose

Expresses V. cholerae CAST TniQ, Cas8, Cas7, and Cas6 from a T7 promoter, with N-terminal His10-MBP-TEVsite tag on TniQ for purification.

Depositor

Samuel H. Sternberg

Article

Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

Insert

VchTniQ, VchCas8, VchCas7, VchCas6

Use

CRISPR; Transposon

Tags

His10-MBP-TEVsite on TniQ

Expression

Bacterial

Promoter

T7

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pMRX-IP-EGFP-mouse Plaat3 (N157stop)

Plasmid

#168771

Purpose

Expresses EGFP-Plaat3 (C-terminal element is deleted) in mammalian cells

Depositor

Noboru Mizushima

Article

Morishita et al Nature. 2021 Apr;592(7855):634-638. doi:

Insert

Hrasls3 (Plaat3 Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Available Since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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PresentER-NLVPMVATV (GFP)

Plasmid

#102947

Purpose

PresentER minigene encoding HLA-A*02:01 ligand NLVPMVATV (GFP)

Depositor

David Scheinberg

Article

Gejman et al Cancer Immunol Res. 2020 May;8(5):672-68

Insert

NLVPMVATV

Use

Retroviral

Expression

Mammalian

Promoter

LTR

Available Since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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NO lux Sensor

Plasmid

#199790

Purpose

Synthetic gene circuit

Depositor

Timothy Lu

Article

Inda-Webb et al Nature. 2023 Aug;620(7973):386-392. doi:

Insert

NO lux Sensor

Use

Synthetic Biology

Available Since

July 26, 2023

Availability

Academic Institutions and Nonprofits only

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pCH4 (denAsCas12a-KRAB lenti)

Plasmid

#217331

Purpose

Lentiviral expression of denAsCas12a-KRAB

Depositor

Luke Gilbert

Article

Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

Insert

denAsCas12a

Use

CRISPR and Lentiviral

Tags

6xMycNLS and HA-SV40NLS-XTEN80-KRAB-P2A-TagBFP2

Mutation

D908A/E174R/S542R/K548R

Promoter

UCOE-SFFV

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pSR358.4

Plasmid

#125114

Purpose

Expression of narL(1-159aa)-liaR(154-211aa) chimera under PLtetO-1, output Promoter PliaI121

Depositor

Jeffrey Tabor

Article

Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

Inserts

narL(REC)-liaR(DBD)159
sfgfp
tetR

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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[pSK467] pLJC5 HA-Depdc5

Plasmid

#109343

Purpose

Lenti-viral expression of HA-tagged wildtype Depdc5

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Nature. 2018 Apr 5;556(7699):64-69. doi:

Insert

Depdc5 (DEPDC5 Human)

Use

Lentiviral

Tags

HA

Available Since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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rnLbCas12a-PE2

Plasmid

#213746

Purpose

For circular RNA-mediated prime editor using rnLbCas12a-PE2 in HEK293T cells

Depositor

Caixia Gao

Article

Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

Insert

rnLbCas12a-M-MLV RT∆RNase H

Use

CRISPR

Tags

BPNLS

Expression

Mammalian

Mutation

D156R, H759A, R1138A in LbCas12a

Promoter

CMV

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pSCALPS-Flag-Miwi-EGFP

Plasmid

#212575

Purpose

Lentiviral expression of Flag-tagged mouse Miwi in mammalian cells

Depositor

Phillip Zamore

Article

Arif et al Nature. 2022 Aug;608(7923):618-625. doi:

Insert

Miwi (Piwil1 Mouse)

Use

Lentiviral

Tags

5x Flag and SNAP tag

Expression

Mammalian

Promoter

SFFV

Available Since

May 16, 2024

Availability

Academic Institutions and Nonprofits only

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pET26b_AT118-L-V5-His

Plasmid

#222848

Purpose

E. coli periplasmid expression plasmid encoding Nb. AT118-L, a humanized nanobody antagonist of the human Angiotensin II type I receptor (AT1R) with reduced polyreactivity

Depositor

Andrew Kruse

Article

Skiba et al Nat Chem Biol. 2024 May 14. doi: 10.1038

Insert

AT118-L

Tags

V5-His

Expression

Bacterial

Available Since

Sept. 12, 2024

Availability

Academic Institutions and Nonprofits only

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PZac2.1 hSyn1-Lck-13xLinker-BioID2-BioID2-HA

Plasmid

#176854

Purpose

Expresses membrane bound biotin ligase, BioID2, in neurons

Depositor

Baljit Khakh

Article

Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

Insert

Lck-13xLinker-BioID2-BioID2-HA

Use

AAV

Tags

HA

Expression

Bacterial

Promoter

hSyn1

Available Since

April 11, 2023

Availability

Academic Institutions and Nonprofits only

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pSL0828 (pQCascade_crRNA-4)

Plasmid

#130637

Purpose

Expresses V. cholerae CAST TniQ, Cas8, Cas7, and Cas6 from one T7 promoter, and a CRISPR RNA from a second T7 promoter. The CRISPR array encodes a guide RNA that targets lacZ.

Depositor

Samuel H. Sternberg

Article

Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

Insert

VchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(target-4)

Use

CRISPR; Transposon

Expression

Bacterial

Promoter

T7

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pLKO.1-Tet-Puro-shIAPEz_2

Plasmid

#185017

Purpose

shRNA mediated knockdown

Depositor

Denes Hnisz

Article

Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

Insert

ERV_IAPEz

Use

Lentiviral

Available Since

July 18, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO.1-Tet-Puro-shIAPEz_1

Plasmid

#185016

Purpose

shRNA mediated knockdown

Depositor

Denes Hnisz

Article

Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

Insert

ERV_IAPEz

Use

Lentiviral

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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