We narrowed to 24,197 results for: nsf

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pDONR221-ARL8B

Plasmid

#67334

Purpose

hArl8b with stop codon in Gateway Entry vector

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL8B (ARL8B Human)

Use

Gateway entry vector

Expression

Bacterial

Promoter

None

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pCAG-hNme2Cas9-NLS(SV40)-3xFLAG (KAC582)

Plasmid

#134331

Purpose

CAG promoter expression plasmid for human codon optimized Nme2Cas9 nuclease with C-terminal NLS (SV40) and 3x FLAG tag

Depositor

Joseph Bondy-Denomy, Benjamin Kleinstiver

Article

Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

Insert

human codon optimized Nme2Cas9

Tags

NLS(SV40)-3xFLAG

Expression

Mammalian

Promoter

CAG

Available Since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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ALARM NMR

Plasmid

#90209

Purpose

Expresses the human La antigen for use in ALARM NMR

Depositor

Michael Walters

Article

Dahlin et al Nat Commun. 2017 Nov 15;8(1):1527. doi:

Insert

La antigen (SSB Human)

Tags

6xHis

Expression

Bacterial

Mutation

contains amino acids 100-324

Promoter

T7 promoter

Available Since

Feb. 12, 2018

Availability

Academic Institutions and Nonprofits only

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SpIMPDH

Plasmid

#206980

Purpose

Streptococcus pyogenes Inosine 5′-Monophosphate Dehydrogenase (SpIMPDH, EC. 1.1.1.205). Contains His6 tag with TEV protease cleavage site in pET-28b(+) plasmid for protein expression.

Depositor

Vern Schramm

Article

Glockzin et al Biochemistry. 2022 Oct 4;61(19):2088-210

Insert

SpIMPDH (guaB Streptococcus pyogenes)

Tags

His tag (with TEV cleavage site)

Expression

Bacterial

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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GFP-IP3KC

Plasmid

#134603

Purpose

Expresses IP3KC GFP tagged

Depositor

Christophe Erneux

Article

Dewaste et al Biochem J. 2003 Aug 15;374(Pt 1):41-9.

Insert

IP3KC (ITPKC Human)

Tags

GFP

Expression

Mammalian

Available Since

Nov. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pD21-hgAPOAI

Plasmid

#124962

Purpose

Express ApoAI in the liver

Depositor

Kazuhiro Oka

Article

Belalcazar et al Circulation. 2003 Jun 3;107(21):2726-32.

Insert

human APOAI gene (LPA Human)

Use

Adenoviral

Promoter

human APOAI

Available Since

July 19, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mGnptg

Plasmid

#78106

Purpose

Mammalian expression of mouse Gnptg with C-terminal His tag

Depositor

Thomas Braulke

Article

Encarnacao et al J Biol Chem. 2011 Feb 18;286(7):5311-8.

Insert

Gnptg (Gnptg Mouse)

Tags

His

Expression

Mammalian

Promoter

CMV

Available Since

May 19, 2016

Availability

Academic Institutions and Nonprofits only
pT2ADW-lox-mCherryNLS-pA-lox-4493NES

Plasmid

#138374

Purpose

Booster-PKA for the generation of mice

Depositor

Michiyuki Matsuda

Article

Watabe et al ACS Sens. 2020 Feb 26. doi: 10.1021/acss

Insert

lox-mCherry-lox-Booster-PKA

Expression

Mammalian

Promoter

CAG

Available Since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV1-SMAC-HA-eGFP

Plasmid

#67487

Purpose

Targets HA-eGFP to mitochondrial intermembrane space (IMS)

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

SMAC (DIABLO Human)

Tags

HA-eGFP

Expression

Mammalian

Mutation

aa1-59

Promoter

attenuated CMV

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pBV-Luc/Del-6

Plasmid

#14969

Depositor

Joan Massague

Article

Chen et al Proc Natl Acad Sci U S A. 2001 Jan 30. 9

Insert

c-myc promoter (-109/+334) (MYC Human)

Use

Luciferase

Expression

Mammalian

Mutation

-109/+334 (relative to the P2 transcription initi…

Available Since

May 17, 2007

Availability

Academic Institutions and Nonprofits only
FN-Ets2

Plasmid

#44603

Depositor

Robert Oshima

Article

Galang et al J Biol Chem. 1996 Apr 5;271(14):7992-8.

Insert

Ets2 (Ets2 Mouse)

Tags

FLAG and SV40 NLS

Expression

Mammalian

Promoter

CMV

Available Since

April 26, 2013

Availability

Academic Institutions and Nonprofits only

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gh136

Plasmid

#106806

Purpose

expression of gRNA targeting EXT2

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

EXT2 (EXT2 Human)

Expression

Mammalian

Available Since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a-FAM86A

Plasmid

#85117

Purpose

bacterial expression of FAM86A

Depositor

Pal Falnes

Article

Davydova et al J Biol Chem. 2014 Oct 31;289(44):30499-5

Insert

EEF2KMT (FAM86A Human)

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pDEST40-ARF6-V5-His6

Plasmid

#67436

Purpose

Mammalian expression of hArf6 with C-term V5 and His6 tag

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARF6 (ARF6 Human)

Tags

His and V5

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
(194) pcDNA3.1-HA-nEPEA-(EAAAK)4-OGT(4,K852A)

Plasmid

#160742

Purpose

Expresses an inactive truncated OGT (4.5 TPRs, K852A) that can't bind O-GlcNAc fused to the EPEA nanobody. A different nanobody can be inserted by using Sgfl + Sgsl restriction enzymes.

Depositor

Christina Woo

Article

Ramirez et al ACS Chem Biol. 2020 Apr 17;15(4):1059-10

Insert

HA-nEPEA-(EAAAK)4-OGT(4,K852A)

Tags

HA-Tag

Expression

Mammalian

Promoter

T7/CMV

Available Since

May 25, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV5-Flag-EPLIN delta N-term

Plasmid

#40932

Depositor

Elizabeth Luna, Scott Maul

Article

Song et al Mol Biol Cell. 2002 Apr;13(4):1408-16.

Insert

EPLIN (LIMA1 Human)

Tags

Flag

Expression

Mammalian

Mutation

deleted amino acids 1-220

Available Since

Dec. 3, 2012

Availability

Academic Institutions and Nonprofits only
pCMV5-Flag-EPLIN alpha delta C-term

Plasmid

#40930

Depositor

Elizabeth Luna, Scott Maul

Article

Song et al Mol Biol Cell. 2002 Apr;13(4):1408-16.

Insert

EPLIN alpha (LIMA1 Human)

Tags

Flag

Expression

Mammalian

Mutation

deleted amino acids 296-600

Available Since

Dec. 12, 2012

Availability

Academic Institutions and Nonprofits only
pONSY-CoNMM:mCherry

Plasmid

#111878

Purpose

This plasmid expresses mCherry fluorescent protein fused to an N-Myristoylation motif (NMM) from the endogenous Src2 gene (CAOG_06360). It can be used to visualize the membrane and filopodia.

Depositor

Inaki Ruiz-Trillo

Article

Parra-Acero et al Development. 2018 May 11. pii: dev.16210

Insert

Capsaspora N-Myristoylation motif (CoNMM) from the Src2 gene fused to mCherry

Use

Capsaspora owczarzaki

Tags

mCherry

Promoter

Elongation Factor 1 alpha (EF1a) from Capsaspora

Available Since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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pET-DEST42-ARL8B-V5-His6

Plasmid

#67425

Purpose

Expression of hArl8b with C-term V5 and His6 tag in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL8B (ARL8B Human)

Tags

His and V5

Expression

Bacterial

Promoter

T7/lacO

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pET-DEST42-ARF1-V5-His6

Plasmid

#67411

Purpose

Expression of hArf1 with C-term V5 and His6 tag in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARF1 (ARF1 Human)

Tags

His and V5

Expression

Bacterial

Promoter

T7/lacO

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only