We narrowed to 21,503 results for: KIN

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pSEED-Moontag:HA

Plasmid

#219648

Purpose

Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in Moontag and HAtag using the SEED/Harvest method

Depositor

Markus Affolter

Article

Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

Insert

MoonTag:HA SEED Cassette

Available Since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pFA6a-BASU-HA3-KanMX6

Plasmid

#227978

Purpose

To insert biotin ligase, BASU, at the C-terminus of genes; yeast BioID, KanMX6 marker

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

BASU

Tags

HA tag

Expression

Yeast

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pFA6a-BioID2-HA3-KanMX6

Plasmid

#227976

Purpose

To insert HA-tagged biotin ligase, BioID2, at the C-terminus of genes; yeast BioID, KanMX6 marker

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

BioID2

Tags

HA tag

Expression

Yeast

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pFA6a-BirA-HA3-KanMX6

Plasmid

#227974

Purpose

To insert HA-tagged biotin ligase, BirA, at the C-terminus of genes; yeast BioID with KanMX6 marker

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

BirA

Tags

HA tag

Expression

Yeast

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pDest17_A1-LCD swap X10

Plasmid

#226552

Purpose

Bacterial expression of N-terminally 6His tagged A1-LCD X10

Depositor

Tanja Mittag

Article

Pesce et al Sci Adv. 2024 Aug 30;10(35):eadm9926. do

Insert

A1-LCD swap X10

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pDest17_A1-LCD swap X9

Plasmid

#226551

Purpose

Bacterial expression of N-terminally 6His tagged A1-LCD X9

Depositor

Tanja Mittag

Article

Pesce et al Sci Adv. 2024 Aug 30;10(35):eadm9926. do

Insert

A1-LCD swap X9

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pDest17_A1-LCD swap X8

Plasmid

#226550

Purpose

Bacterial expression of N-terminally 6His tagged A1-LCD X8

Depositor

Tanja Mittag

Article

Pesce et al Sci Adv. 2024 Aug 30;10(35):eadm9926. do

Insert

A1-LCD swap X8

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pDest17_A1-LCD swap X6

Plasmid

#226548

Purpose

Bacterial expression of N-terminally 6His tagged A1-LCD X6

Depositor

Tanja Mittag

Article

Pesce et al Sci Adv. 2024 Aug 30;10(35):eadm9926. do

Insert

A1-LCD swap X6

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pDest17_A1-LCD swap X2

Plasmid

#226544

Purpose

Bacterial expression of N-terminally 6His tagged A1-LCD X2

Depositor

Tanja Mittag

Article

Pesce et al Sci Adv. 2024 Aug 30;10(35):eadm9926. do

Insert

A1-LCD swap X2

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pSEED-EYFP:HA

Plasmid

#219656

Purpose

Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in EYFP and HA tag using the SEED/Harvest method

Depositor

Markus Affolter

Article

Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

Insert

EYFP:HA SEED Cassette

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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p2T_CAG_SpCas9PE2_Igf1-NFATC2IP_BlastR (pepPE2_3)

Plasmid

#173067

Purpose

Expresses NI-PE2

Depositor

Richard Sherwood

Article

Velimirovic et al Nat Commun. 2022 Jun 18;13(1):3512. doi:

Insert

Igf1-NFATC2IP

Expression

Mammalian

Available Since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pD(P+T)_CMVN

Plasmid

#223861

Purpose

4G-cloning donor for mammalian promoter and terminator, N-terminal tag added

Depositor

Stephan Gruber

Article

Taschner et al Life Sci Alliance. 2024 Dec 2;8(1):e2024

Insert

CMV promoter and bGH polyA

Use

Golden-gate donor

Available Since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pAM284

Plasmid

#226122

Purpose

Expression of cdc48 E588Q

Depositor

Andreas Martin

Article

Williams et al Mol Cell. 2023 Mar 2;83(5):759-769.e7. d

Insert

His6-3C-Cdc48 E588Q (CDC48 Budding Yeast)

Expression

Bacterial

Mutation

E588Q

Available Since

Sept. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pAM290

Plasmid

#226127

Purpose

Expression of His6-GST-3C-Npl4

Depositor

Andreas Martin

Article

Williams et al Mol Cell. 2023 Mar 2;83(5):759-769.e7. d

Insert

His6-GST-3C-Npl4 (HIS6 Budding Yeast)

Expression

Bacterial

Available Since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pAM289

Plasmid

#226126

Purpose

Expression of Npl4 D460K/Y461A

Depositor

Andreas Martin

Article

Williams et al Mol Cell. 2023 Mar 2;83(5):759-769.e7. d

Insert

Npl4 D460K/Y461A (NPL4 Budding Yeast)

Expression

Bacterial

Mutation

D460K/Y461A

Available Since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pAM358

Plasmid

#226138

Purpose

Expression of His6-SUMO-Ub-mEos3.2-mTurqoise2-intein-CBD

Depositor

Andreas Martin

Article

Williams et al Mol Cell. 2023 Mar 2;83(5):759-769.e7. d

Insert

His6-SUMO-Ub-mEos3.2-mTurqoise2-intein-CBD

Expression

Bacterial

Available Since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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paM291

Plasmid

#226128

Purpose

Expression of His6-GST-3C-Npl4 T255A/T571A

Depositor

Andreas Martin

Article

Williams et al Mol Cell. 2023 Mar 2;83(5):759-769.e7. d

Insert

His6-GST-3C-Npl4 T255A/T571A (HIS6 Budding Yeast)

Expression

Bacterial

Mutation

T255A/T571A

Available Since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pAM288

Plasmid

#226125

Purpose

Expression of Npl4 W252A/R253E

Depositor

Andreas Martin

Article

Williams et al Mol Cell. 2023 Mar 2;83(5):759-769.e7. d

Insert

Npl4 W252A/R253E (NPL4 Budding Yeast)

Expression

Bacterial

Mutation

W252A/R253E

Available Since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pAM285

Plasmid

#226123

Purpose

Expression of cdc48 W561A/Y562A

Depositor

Andreas Martin

Article

Williams et al Mol Cell. 2023 Mar 2;83(5):759-769.e7. d

Insert

His6-3C-Cdc48 W561A/Y562A (CDC48 Budding Yeast)

Expression

Bacterial

Mutation

W561A/Y562A

Available Since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pD(P+T)_phN

Plasmid

#223858

Purpose

4G-cloning donor for insect promoter and terminator, N-terminal tag added

Depositor

Stephan Gruber

Article

Taschner et al Life Sci Alliance. 2024 Dec 2;8(1):e2024

Insert

polyhedrin promoter and SV40 polyA

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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