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Showing: 801 - 820 of 4219 results

  4. GPER-DuET

    Plasmid
    #213256
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    GPER (GPER1 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213256-plasmid-map-sequence-id-430806

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  7. pD649-HAsp-TNFSF13-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156611
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    TNFSF13 (TNFSF13 Human)
    Use
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available since
    March 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    156611-plasmid-map-sequence-id-426830

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  8. N295A (EcN) MetNIQ in pBAD

    Plasmid
    #118256
    Purpose
    N295A Mutation of MetN in Methionine ABC Transporter and Wild Type Methionine Binding Protein for Transport Activity Assay
    Depositor
    Douglas Rees
    Article
    Nguyen et al Proc Natl Acad Sci U S A. 2018 Oct 23. p
    Inserts
    MetN (N295A) (metN E. coli)
    MetI (metI E. coli)
    MetQ (metQ E. coli)
    Use
    Tags
    10x HIs Tag, 6x His Tag, and Enterokinase Cleavag…
    Expression
    Bacterial
    Mutation
    Changed Asparagine (Asn) 295 to Alanine (Ala)
    Promoter
    None and araBAD Promotoer
    Available since
    Nov. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    118256-plasmid-map-sequence-id-228530

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  10. pLKO_VSVg.AU1.C_NGFR

    Plasmid
    #158256
    Purpose
    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).
    Depositor
    Brian Brown
    Article
    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d
    Insert
    Pro-Code Tagged human dNGFR (NGFR Human)
    Use
    CRISPR and Lentiviral
    Tags
    VSVg.AU1.C
    Expression
    Mutation
    Truncation of the signaling domain
    Promoter
    EF1a
    Available since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    158256-plasmid-map-sequence-id-334984

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  11. pcDNA3.1-N-Myc_DW164AA + DED/AAA USP7

    Plasmid
    #131256
    Purpose
    Mammalian expression of N-terminally Myc-tagged USP7, with mutations in the TRAF-like domain (DW164AA) and UBL2 (DE758AA_D764A)
    Depositor
    Roger Woodgate
    Article
    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d
    Insert
    Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Contains point mutations that convert USP7 aspart…
    Promoter
    Available since
    Dec. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    131256-plasmid-map-sequence-id-267711

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  13. 18065-M01-412

    Plasmid
    #225664
    Purpose
    Lentiviral expression of FLAG-tagged fluorescent proteins for immunohistochemical detection in FFPE tissue
    Depositor
    Dominic Esposito
    Article
    Sharma et al Nat Commun. 2021 Dec 15;12(1):7300. doi:
    Inserts
    dsCopGFP
    delCD19 (CD19 Human)
    Use
    Lentiviral
    Tags
    3xFLAG
    Expression
    Mutation
    C-terminally truncated (aa 1-333 only)
    Promoter
    SORE6-mCMVp and WPRE-SV40p
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    225664-plasmid-map-sequence-id-452346

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  14. 18065-M02-412

    Plasmid
    #225665
    Purpose
    Lentiviral expression of FLAG-tagged fluorescent proteins for immunohistochemical detection in FFPE tissue
    Depositor
    Dominic Esposito
    Article
    Sharma et al Nat Commun. 2021 Dec 15;12(1):7300. doi:
    Inserts
    dsmCherry
    delCD19 (CD19 Human)
    Use
    Lentiviral
    Tags
    3xFLAG
    Expression
    Mutation
    C-terminally truncated (aa 1-333 only)
    Promoter
    SORE6-mCMVp and WPRE-SV40p
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    225665-plasmid-map-sequence-id-452347

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  16. pEMS1526

    Plasmid
    #29256
    Purpose
    Pleiades (Ple) MiniPromoter expression pattern described in Portales-Casamar et al., PNAS, 2010 (PMID: 20807748) and de Leeuw et al., MTM, 2014 (PMID: 24761428).
    Depositor
    Elizabeth Simpson
    Article
    Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1
    Insert
    Ple55 (DCX Human)
    Use
    Pleiades promoter project [sic, pleaides plieades]
    Tags
    Intron-LacZ-NLS
    Expression
    Mutation
    Promoter
    Available since
    Nov. 7, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits
    29256-plasmid-map-sequence-id-13671

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  18. Anti-Alpha-Tubulin [F2C] - Chimeric (Recombinant Antibody)

    Antibody
    #198269-rAb
    Purpose
    Anti-Alpha-Tubulin chimeric recombinant antibody. The hybridoma-derived heavy chain variable sequence and kappa light chain are fused to a rabbit IgG Fc.
    Depositor
    Melina Fan
    Recommended Applications
    Immunocytochemistry
    Reactivity
    Human
    Source Species
    Rabbit
    Isotype
    IgG
    Trial Size
    Available to purchase
    Available since
    April 3, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    Immunocytochemistry image by Addgene

  20. pBABE puro human RXR alpha

    Plasmid
    #11441
    Depositor
    Ronald Kahn
    Insert
    RXR alpha (RXRA Human)
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 3, 2006
    Availability
    Academic Institutions and Nonprofits only
Showing: 801 - 820 of 4219 results