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We narrowed to 2,493 results for: D10

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Showing: 801 - 820 of 2493 results

  1. pUF1-dCas9-GCN5

    Plasmid
    #122509
    Purpose
    Combining with the specific sgRNA sequence to activate of the Plasmodium falciparum endogenous gene transcription, through the increasing of H3K9 acetylation.
    Depositor
    Lubin Jiang
    Publication
    Xiao et al Proc Natl Acad Sci U S A. 2019 Jan 2;116
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9-GCN5
    Use
    CRISPR
    Tags
    3*FLAG
    Mutation
    dCas9(D10A,H840A)
    Promoter
    PfHsp86
    Available since
    June 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    122509-plasmid-map-sequence-id-245888

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  2. pX335-EN475

    Plasmid
    #86231
    Purpose
    For transient expression of spCas9-nickase and one sgRNA targeting the mouse CTCF locus. Use together with pX335-EN477.
    Depositor
    Benoit Bruneau
    Publication
    Nora et al Cell. 2017 May 18;169(5):930-944.e22. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    spCas9-nickase and sgRNA against mouse CTCF STOP Codon
    Use
    Mouse Targeting
    Expression
    Mammalian
    Available since
    May 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    86231-plasmid-map-sequence-id-177755

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  3. pAWPSG-Po-nCas9-BE- Ptac-sgRNA(mmoX_1)

    Plasmid
    #195741
    Purpose
    Base editing for making a pre-stop codon in mmoX using phenol inducible system
    Depositor
    Seung-Goo Lee
    Publication
    Jeong et al Chem Eng J, 2023
    Insert
    Di-Methyl Phenol Regulatory protein / APOBEC1-nCas9(D10A)-UGI / Ptac-RiboJ-sgRNA(mmoX_1)
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    Po (phoenol-inducible promoter)
    Available since
    March 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    195741-plasmid-map-sequence-id-386423

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  6. pJ609-dCas9-KR-puro

    Plasmid
    #158478
    Purpose
    Express nuclease dead Cas9 fused to KillerRed chromophore in mammalian cells
    Depositor
    Brendan Price
    Publication
    House et al PLoS One. 2020 Dec 17;15(12):e0237759. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9-KR
    Use
    CRISPR
    Tags
    KillerRed
    Expression
    Mammalian
    Mutation
    Cas9m4 (D10A+D839A+H840A+N863A)
    Promoter
    CMV
    Available since
    Nov. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    158478-plasmid-map-sequence-id-317143

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  10. pEF_dCas9-VP64

    Plasmid
    #68417
    Purpose
    Transient expression of Sp-dCas9 fused to the VP64 transcription activator, in mammalian cells, under an EF1-alpha promoter.
    Depositor
    John Rinn
    Publication
    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9
    Use
    CRISPR
    Tags
    3xFLAG and VP64
    Expression
    Mammalian
    Mutation
    D10A/H841A
    Promoter
    human EF1[alpha]
    Available since
    Sept. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    68417-plasmid-map-sequence-id-120195

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  11. pCMV-hA3A-eBE-Y132D

    Plasmid
    #113429
    Purpose
    Expresses hA3A-eBE-Y132D in mammalian cells
    Depositor
    Jia Chen
    Publication
    Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    hA3A-eBE-Y132D (APOBEC3A Human, S. pyogenes and Bacteriophage PBS2)
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    hAPOBEC3A_Y132D
    Promoter
    CMV
    Available since
    Aug. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    113429-plasmid-map-sequence-id-217446

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  14. A3A-PBE

    Plasmid
    #119768
    Purpose
    Conversion of C to T in wheat plants or monocotyledons protoplasts
    Depositor
    Caixia Gao
    Publication
    Zong et al Nat Biotechnol. 2018 Oct 1. pii: nbt.426
    Insert
    APOBEC3A-nCas9-NLS-UGI-NLS
    Tags
    NLS (flanking UGI)
    Expression
    Plant
    Mutation
    nCas9 has a mutation D10A and E945K in wild type …
    Promoter
    maize Ubiquitin-1
    Available since
    June 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    119768-plasmid-map-sequence-id-232090

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  16. miniCGBE1-NG (pBM1182)

    Plasmid
    #140257
    Purpose
    CMV promoter expression plasmid for rAPOBEC1(R33A)-nCas9_NG-P2A-EGFP
    Depositor
    Keith Joung
    Publication
    Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    BE4max(R33A)_NG-ΔUGI
    Expression
    Mammalian
    Mutation
    R33A in rAPOBEC1, NG mutations in SpCas9, D10A in…
    Promoter
    CMV
    Available since
    Aug. 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    140257-plasmid-map-sequence-id-291773

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  18. pX335-NEAT1_IS_v2

    Plasmid
    #97086
    Purpose
    Encodes an sgRNA that creates a SSB near the poly(A) site of human NEAT1_1 isoform.
    Depositor
    Archa Fox
    Publication
    Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgRNA NEAT1_IS_v2
    Use
    CRISPR
    Promoter
    U6
    Available since
    July 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    97086-plasmid-map-sequence-id-178717

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  19. pX335-NEAT1_IS_v1

    Plasmid
    #97085
    Purpose
    Encodes an sgRNA that creates a SSB near the poly(A) site of human NEAT1_1 isoform.
    Depositor
    Archa Fox
    Publication
    Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgRNA NEAT1_IS_v1
    Use
    CRISPR
    Promoter
    U6
    Available since
    July 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    97085-plasmid-map-sequence-id-189638

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  20. pX335-EN477

    Plasmid
    #86232
    Purpose
    For transient expression of spCas9-nickase and one sgRNA targeting the mouse CTCF locus. Use together with pX335-EN475.
    Depositor
    Benoit Bruneau
    Publication
    Nora et al Cell. 2017 May 18;169(5):930-944.e22. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    spCas9-nickase and sgRNA against mouse CTCF STOP Codon
    Use
    Mouse Targeting
    Expression
    Mammalian
    Available since
    May 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    86232-plasmid-map-sequence-id-177242

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Showing: 801 - 820 of 2493 results