We narrowed to 2,242 results for: D10

1. pET-Hisx6-rAPOBEC1-XTEN-nCas9-UGI-NLS

   Plasmid

   #89508

   Purpose

   Expression of Hisx6-rAPOBEC1-XTEN-nCas9-UGI-NLS in bacterial cells.

   Depositor

   Jin-Soo Kim

   Article

   Kim et al Nat Biotechnol. 2017 Feb 27. doi: 10.103

   Insert

   rAPOBEC1-nCas9 (D10A)-UGI-NLS
   

   Tags

   UGI and rAPOBEC-XTEN

   Expression

   Bacterial

   Mutation

   D10A mutation of Cas9

   Promoter

   T7

   Available Since

   May 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. MO91-dCas9-AID(P182X)

   Plasmid

   #83260

   Purpose

   Expresses fusion of human codon-optimized S. pyogenes dCas9 (D10A, H840A ) protein with a human AID mutant protein (p182X). MSCV Retroviral backbone.

   Depositor

   Xing Chang

   Article

   Ma et al Nat Methods. 2016 Dec;13(12):1029-1035.

   Insert

   dCas9-AID P182X
   

   Use

   Retroviral

   Tags

   3xFLAG-NLS and FLAG

   Expression

   Mammalian

   Mutation

   D10A and H840A in Cas9; P182X in AID

   Promoter

   MSCV

   Available Since

   May 4, 2017

   Availability

   Academic Institutions and Nonprofits only

3. miniABEmax (pRZ900)

   Plasmid

   #131311

   Purpose

   CMV promoter expression plasmid for bpNLS-TadA7.10-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS (ABEmax with truncation of WT TadA domain).

   Depositor

   Keith Joung

   Article

   Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038

   Insert

   bpNLS-TadA7.10-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pH-Ubi-miniSdd7-nCas9-PBE

   Plasmid

   #204856

   Purpose

   For cytosine base editing using miniSdd7 in monocotyledon plants (Agrobacterium-mediated transformation)

   Depositor

   Caixia Gao

   Article

   Huang et al Cell. 2023 Jul 20;186(15):3182-3195.e14.

   Insert

   miniSdd7-nSpCas9(D10A)-UGI
   

   Use

   CRISPR

   Expression

   Plant

   Mutation

   D10A in SpCas9

   Promoter

   ZmUbi-1, OsU3, 35S

   Available Since

   Aug. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMonAID_nCas9-PmCDA_Hyg_ALS

   Plasmid

   #91693

   Purpose

   Monocot Target-AID vector expressing rice-optimized nCas9-PmCDA1 with sgRNA targeting OsAls

   Depositor

   Akihiko Kondo

   Article

   Shimatani et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

   Insert

   SpCas9
   

   Tags

   PmCDA1

   Expression

   Plant

   Mutation

   D10A for nickase Cas9

   Promoter

   2x35S

   Available Since

   June 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pYPQ262B-ABE8e

   Plasmid

   #161524

   Purpose

   Gateway entry clone (attL1 & attR5) for CRISPR-zSpRYCas9 ABE8e mediated A-G base editing

   Depositor

   Yiping Qi

   Article

   Ren et al Nat Plants. 2021 Jan 4. pii: 10.1038/s41

   Insert

   TadA8e-zSpRYCas9(D10A)
   

   Use

   CRISPR; Gateway compatible tada8e-zsprycas9(d10a)…

   Expression

   Plant

   Available Since

   Feb. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pYPQ266E

   Plasmid

   #161521

   Purpose

   Gateway entry clone (attL1 & attR5) for CRISPR-zSpRYCas9 PmCDA1 mediated C-T base editing

   Depositor

   Yiping Qi

   Article

   Ren et al Nat Plants. 2021 Jan 4. pii: 10.1038/s41

   Insert

   zSpRYCas9(D10A)-PmCDA1-UGI
   

   Use

   CRISPR; Gateway compatible zsprycas9(d10a)-pmcda1…

   Tags

   3X FLAG, NLS

   Expression

   Plant

   Available Since

   Feb. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAC149-pCR8-dCas9VP160

   Plasmid

   #48221

   Purpose

   dCas9VP160 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expression

   Depositor

   Rudolf Jaenisch

   Article

   Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

   Insert

   dCas9(D10A;H840A) fusion with VP160 activation domain
   

   Use

   CRISPR; Gateway donor

   Tags

   HA Tag and VP160

   Mutation

   D10A;H840A nuclease-deficient

   Available Since

   Sept. 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMonAID_dCas9-PmCDA_Hyg_ALS

   Plasmid

   #91692

   Purpose

   Monocot Target-AID vector expressing rice-optimized dCas9-PmCDA1 with sgRNA targeting OsAls

   Depositor

   Akihiko Kondo

   Article

   Shimatani et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

   Insert

   SpCas9
   

   Tags

   PmCDA1

   Expression

   Plant

   Mutation

   D10A and H840A for dead Cas9

   Promoter

   2x35S

   Available Since

   June 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDEST_6xUAS:Lamp1-mGFP

    Plasmid

    #192356

    Purpose

    Lamp1 tagged with mGFP, expressed under the UAS promoter

    Depositor

    Francesca Peri

    Article

    Moller et al Elife. 2022 Nov 18;11:e82094. doi: 10.75

    Insert

    Lamp1 (LAMP1 Human)
    

    Tags

    mGFP

    Expression

    Mammalian

    Available Since

    Dec. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAC147-pCR8-dCas9VP64

    Plasmid

    #48219

    Purpose

    dCas9VP64 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expression 

    Depositor

    Rudolf Jaenisch

    Article

    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

    Insert

    dCas9(D10A;H840A) fusion with VP64 activation domain
    

    Use

    CRISPR; Gateway donor

    Tags

    HA Tag and VP64

    Mutation

    D10A;H840A

    Available Since

    Sept. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. Sema4d-Fc-His

    Plasmid

    #72157

    Purpose

    Expresses the extracellular region of the Sema4D protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Sema4d (Sema4d Mouse)
    

    Tags

    Fc-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAT15546_nABE8e-SuperFi-Cas9

    Plasmid

    #184376

    Purpose

    Mammalian expression of SuperFi-Cas9 ABE8e base editor

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2022 Nov 11;13(1):6858. doi:

    Insert

    nABE8e-SuperFi-Cas9
    

    Use

    CRISPR

    Tags

    Nucleoplasmin NLS and SV40 NLS

    Expression

    Mammalian

    Mutation

    D10A, Y1010D, Y1013D, Y1016D, V1018D, R1019D, Q10…

    Promoter

    EF-1α core promoter

    Available Since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pYPQ262m

    Plasmid

    #161522

    Purpose

    Gateway entry clone (attL1 & attR5) for CRISPR-zSpCas9 ABE7.10 mediated A-G base editing

    Depositor

    Yiping Qi

    Article

    Ren et al Nat Plants. 2021 Jan 4. pii: 10.1038/s41

    Insert

    TadA(wt)-TadA(7.10)-zSpCas9(D10A)
    

    Use

    CRISPR; Gateway compatible tada(wt)-tada(7.10)-zs…

    Expression

    Plant

    Available Since

    Feb. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAT15542_nCBE-SuperFi-Cas9

    Plasmid

    #184372

    Purpose

    Mammalian expression of SuperFi-Cas9 CBE base editor

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2022 Nov 11;13(1):6858. doi:

    Insert

    nCBE-SuperFi-Cas9
    

    Use

    CRISPR

    Tags

    3X FLAG, SV40 NLS, and UGI

    Expression

    Mammalian

    Mutation

    D10A, Y1010D, Y1013D, Y1016D, V1018D, R1019D, Q10…

    Promoter

    EF-1α core promoter

    Available Since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAT15544_nABE-SuperFi-Cas9

    Plasmid

    #184374

    Purpose

    Mammalian expression of SuperFi-Cas9 ABE7 base editor

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2022 Nov 11;13(1):6858. doi:

    Insert

    nABE-SuperFi-Cas9
    

    Use

    CRISPR

    Tags

    FLAG, SV40 NLS, and nucleoplasmin NLS

    Expression

    Mammalian

    Mutation

    D10A, Y1010D, Y1013D, Y1016D, V1018D, R1019D, Q10…

    Promoter

    EF-1α core promoter

    Available Since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAC148-pCR8-dCas9VP96

    Plasmid

    #48220

    Purpose

    dCas9VP96 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expression

    Depositor

    Rudolf Jaenisch

    Article

    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

    Insert

    dCas9(D10A;H840A) fusion with VP96 activation domain
    

    Use

    CRISPR; Gateway donor

    Tags

    HA Tag and VP96

    Mutation

    D10A;H840A

    Available Since

    Sept. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. Sema4d-AP-His

    Plasmid

    #72031

    Purpose

    Expresses the extracellular region of the Sema4D protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Sema4d (Sema4d Mouse)
    

    Tags

    AP-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. KHBD00416

    Plasmid

    #34523

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG17950 (HmgD Fly)
    

    Use

    Gateway donor vector

    Available Since

    Jan. 24, 2012

    Availability

    Academic Institutions and Nonprofits only

20. pCMV-myc-Sema4D RNAiR

    Plasmid

    #51600

    Purpose

    expresses RNAi-resistant mouse Sema4D with N-terminal myc tag

    Depositor

    Suzanne Paradis

    Article

    Raissi et al Mol Cell Neurosci. 2013 Nov;57:23-32. do

    Insert

    Sema4D (Sema4d Mouse)
    

    Tags

    myc

    Expression

    Mammalian

    Mutation

    silent mutations between amino acids (approx 275-…

    Promoter

    CMV

    Available Since

    April 7, 2014

    Availability

    Academic Institutions and Nonprofits only