Skip to main content
Addgene
Showing: 801 - 820 of 3634 results

  1. pDIRECT_26G

    Plasmid
    #91149
    Purpose
    Direct Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:TaCas9_dead + TaU6:gRNA , Plant Selection: PvUbi2:bar
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    Engineering Reagent: ZmUbi:TaCas9_dead + TaU6:gRNA
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    D10A, H840A
    Promoter
    Available since
    July 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    91149-plasmid-map-sequence-id-182283

    Enlarge

  2. pDIRECT_25G

    Plasmid
    #91144
    Purpose
    Direct Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:TaCas9_dead + TaU6:gRNA , Plant Selection: PvUbi2:hpt II
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    Engineering Reagent: ZmUbi:TaCas9_dead + TaU6:gRNA
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    D10A, H840A
    Promoter
    Available since
    Sept. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    91144-plasmid-map-sequence-id-182272

    Enlarge

  4. pDIRECT_23D

    Plasmid
    #91141
    Purpose
    Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9_dead + CmYLCV:gRNAs with Csy4 spacers, Plant Selection: 2x35S:bar
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    Engineering Reagent: 35S:Csy4-P2A-AtCas9_dead + CmYLCV:gRNAs with Csy4 spacers
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    D10A, H840A
    Promoter
    Available since
    July 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    91141-plasmid-map-sequence-id-182269

    Enlarge

  5. pDIRECT_25I

    Plasmid
    #91146
    Purpose
    Direct Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9_dead + PvUbi1:gRNAs with Csy4 spacers, Plant Selection: PvUbi2:hpt II
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9_dead + PvUbi1:gRNAs with Csy4 spacers
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    D10A, H840A
    Promoter
    Available since
    July 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    91146-plasmid-map-sequence-id-182476

    Enlarge

  6. pMOD_B2121

    Plasmid
    #91065
    Purpose
    Module B, Promoter: GmUbi, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers , Terminator: 35S
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    GmUbi
    Available since
    July 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    91065-plasmid-map-sequence-id-181862

    Enlarge

  7. pMOD_C2516

    Plasmid
    #91084
    Purpose
    Module C, Promoter: At7SL, Gene: Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning), Terminator: Pol III
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    At7SL
    Available since
    July 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    91084-plasmid-map-sequence-id-182048

    Enlarge

  8. pDIRECT_22D

    Plasmid
    #91136
    Purpose
    Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9_dead + CmYLCV:gRNAs with Csy4 spacers, Plant Selection: 2x35S:npt II
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    Engineering Reagent: 35S:Csy4-P2A-AtCas9_dead + CmYLCV:gRNAs with Csy4 spacers
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    D10A, H840A
    Promoter
    Available since
    July 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    91136-plasmid-map-sequence-id-182475

    Enlarge

  9. pDIRECT_21D

    Plasmid
    #91131
    Purpose
    Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9_dead + CmYLCV:gRNAs with Csy4 spacers, Plant Selection: 2x35S:hpt II
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    Engineering Reagent: 35S:Csy4-P2A-AtCas9_dead + CmYLCV:gRNAs with Csy4 spacers
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    D10A, H840A
    Promoter
    Available since
    July 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    91131-plasmid-map-sequence-id-182255

    Enlarge

  10. pMOD_B2519

    Plasmid
    #91076
    Purpose
    Module B, Promoter: OsU3, Gene: Esp3I ccdb cassette for single gRNA spacer cloning, Terminator: Pol III
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    Esp3I ccdb cassette for single gRNA spacer cloning
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    OsU3
    Available since
    July 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    91076-plasmid-map-sequence-id-181904

    Enlarge

  11. pJG488

    Plasmid
    #91196
    Purpose
    WDV replicon T-DNA for gene targeting in wheat scutella, H840A double nickase (nTaCas9_H840A+gUbi8+gUbi1+donor)
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    nTaCas9_H840A+gUbi8+gUbi1+donor
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Sept. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    91196-plasmid-map-sequence-id-182459

    Enlarge

  12. pMOD_C2519

    Plasmid
    #91088
    Purpose
    Module C, Promoter: OsU3, Gene: Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning), Terminator: Pol III
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    OsU3
    Available since
    July 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    91088-plasmid-map-sequence-id-182053

    Enlarge

  13. pMOD_B2000

    Plasmid
    #91059
    Purpose
    Module B, Promoter: none (begins with P2A to be fused to TALEN_1), Gene: TALEN_2 backbone with Esp3I ccdb cassette for repeat cloning, Terminator: HSP
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    TALEN_2 backbone with Esp3I ccdb cassette for repeat cloning
    Use
    TALEN
    Tags
    Expression
    Mutation
    Promoter
    none (begins with P2A to be fused to TALEN_1)
    Available since
    July 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    91059-plasmid-map-sequence-id-181870

    Enlarge

  14. pMOD_B2403

    Plasmid
    #91071
    Purpose
    Module B, Promoter: CmYLCV, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with ribozyme spacers , Terminator: 35S
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    SapI ccdb cassette for cloning multiple gRNA spacers with ribozyme spacers
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    CmYLCV
    Available since
    July 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    91071-plasmid-map-sequence-id-181880

    Enlarge

  17. pMOD_B3200

    Plasmid
    #91079
    Purpose
    Module B, Promoter: none (begins with P2A to be fused to TALE-VPR_1), Gene: TALE-VPR_2 backbone with Esp3I ccdb cassette for repeat cloning, Terminator: HSP
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    TALE-VPR_2 backbone with Esp3I ccdb cassette for repeat cloning
    Use
    TALEN
    Tags
    Expression
    Mutation
    Promoter
    none (begins with P2A to be fused to TALE-VPR_1)
    Available since
    July 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    91079-plasmid-map-sequence-id-182042

    Enlarge

  18. pMOD_B3400

    Plasmid
    #91080
    Purpose
    Module B, Promoter: none (begins with P2A to be fused to TALE-VP64_1), Gene: TALE-VP64_2 backbone with Esp3I ccdb cassette for repeat cloning, Terminator: HSP
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    TALE-VP64_2 backbone with Esp3I ccdb cassette for repeat cloning
    Use
    TALEN
    Tags
    Expression
    Mutation
    Promoter
    none (begins with P2A to be fused to TALE-VP64_1)
    Available since
    July 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    91080-plasmid-map-sequence-id-182043

    Enlarge

  19. pMOD_A3101

    Plasmid
    #91048
    Purpose
    Module A, Promoter: 35S, Gene: TALE-VPR_1 backbone with Esp3I ccdb cassette for repeat cloning, Terminator: none (ends with P2A to be fused to TALE-VPR_ 2)
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    TALE-VPR_1 backbone with Esp3I ccdb cassette for repeat cloning
    Use
    TALEN
    Tags
    Expression
    Mutation
    Promoter
    35S
    Available since
    July 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    91048-plasmid-map-sequence-id-181838

    Enlarge

  20. pMOD_A3301

    Plasmid
    #91049
    Purpose
    Module A, Promoter: 35S, Gene: TALE-VP64_1 backbone with Esp3I ccdb cassette for repeat cloning, Terminator: none (ends with P2A to be fused to TALE-VP64_ 2)
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    TALE-VP64_1 backbone with Esp3I ccdb cassette for repeat cloning
    Use
    TALEN
    Tags
    Expression
    Mutation
    Promoter
    35S
    Available since
    July 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    91049-plasmid-map-sequence-id-181839

    Enlarge

Showing: 801 - 820 of 3634 results