We narrowed to 2,167 results for: PAM

1. pKO023

   Plasmid

   #242923

   Purpose

   Reporter J1-J3(122)-BBa_J23117 with Sth1 PAM

   Depositor

   Jesse Zalatan

   Article

   Kiattisewee et al ACS Synth Biol. 2025 Jul 22. doi: 10.102

   Insert

   J1-J3(122)
   

   Use

   CRISPR and Synthetic Biology

   Promoter

   Bba_J23117

   Available Since

   Sept. 29, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1-CRISPR-Puro-sgRPL3-62

   Plasmid

   #208647

   Purpose

   pcDNA3.1 based plasmid for transient transfection of sgRNA-cas9. Containing sgRNA targeting human RPL3 (GRCh38.p14_Chr22:39312968-39312987), 62 is a number given by www.e-crisp.org.

   Depositor

   Quanfu Ma

   Insert

   sgRNA targeting human RPL3 (ENST00000216146) C-terminal, No.62 (RPL3 Human, Synthetic)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV and U6 in tandem

   Available Since

   Jan. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-CRISPR-Puro-sgRPL3-56

   Plasmid

   #208979

   Purpose

   pcDNA3.1 based plasmid for transient transfection of sgRNA-cas9. Containing sgRNA targeting human RPL3 (GRCh38.p14_Chr22:39312868-39312887), 56 is a number given by www.e-crisp.org.

   Depositor

   Quanfu Ma

   Insert

   sgRNA targeting human RPL3 (ENST00000216146) C-terminal, No.56 (RPL3 Human, Synthetic)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV and U6 in tandem

   Available Since

   Jan. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGL1_3

   Plasmid

   #199035

   Purpose

   Level 1 plasmid, barcoded ORI

   Depositor

   Nili Ostrov, Charlie Gilbert, Cultivarium Tools

   Article

   Gilbert et al bioRxiv 2023.05.19.541510

   Insert

   pAM?1 ORI plus NGS barcode 3
   

   Use

   Synthetic Biology

   Available Since

   June 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. CRISPR-psbA2 point mutation

   Plasmid

   #182929

   Purpose

   point mutation on psbA2 by CRISPR in Synechocystis 6803

   Depositor

   Himadri Pakrasi

   Article

   Ungerer et al Sci Rep. 2016 Dec 21;6:39681. doi: 10.10

   Inserts

   ddcpf1
   gRNA targeting psbA2 in Synechocystis 6803: gatcttcggtcgcttgatctttc
   psbA
   

   Use

   CRISPR

   Mutation

   S264A, and silent mutation to remove PAM site

   Available Since

   April 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. SK-YFP-NM-A

   Plasmid

   #48664

   Purpose

   Bacterial NM repression YFP reporter: protospacer A

   Depositor

   George Church

   Article

   Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

   Insert

   NM prototspacer A/PAM with reporter EYFP
   

   Use

   CRISPR

   Promoter

   lambda pR promoter

   Available Since

   Oct. 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. SK-YFP-TD-A

   Plasmid

   #48666

   Purpose

   Bacterial TD repression YFP reporter: protospacer A

   Depositor

   George Church

   Article

   Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

   Insert

   TD prototspacer A/PAM with reporter EYFP
   

   Use

   CRISPR

   Promoter

   lambda pR promoter

   Available Since

   Oct. 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-FLEX-DTR-GFP

   Plasmid

   #124364

   Purpose

   conditional expression of a diphtheria toxin receptor (DTR)–GFP fusion protein

   Depositor

   Thomas Jessell, Eiman Azim

   Article

   Azim et al Nature. 2014 Apr 17;508(7496):357-63. do

   Has Service

   AAV2

   Insert

   DTR-GFP
   

   Use

   AAV

   Tags

   GFP

   Promoter

   Chicken B-actin

   Available Since

   Oct. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCas9/VRQR-sgRNA (BbsI)

   Plasmid

   #129725

   Purpose

   Expressing SpCas9/VRQR mutant and sgRNA for target sequence with NGA PAM

   Depositor

   Elina Ikonen

   Article

   Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. px459 VQR

    Plasmid

    #101715

    Purpose

    sgRNA/Cas9 expression plasmid with Cas9 VQR mutations (NGA PAM)

    Depositor

    Andrew Holland

    Article

    Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

    Insert

    SpCas9 VQR
    

    Tags

    3xFLAG-NLS and NLS

    Expression

    Mammalian

    Mutation

    VQR (D1135V, R1335Q and T1337R)

    Available Since

    Oct. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMS13: pTarget(AvCAST)

    Plasmid

    #168146

    Purpose

    Target plasmid containing AvPSP1 and attachement site for AvCAST.

    Depositor

    Feng Zhang

    Article

    Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

    Inserts

    PAM for AvCAST + AvPSP1
    glmS
    

    Expression

    Bacterial

    Available Since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. px458 EQR

    Plasmid

    #101731

    Purpose

    Expresses a sgRNA and a Cas9 EQR variant that recognizes "NGAG" PAM motifs

    Depositor

    Andrew Holland

    Article

    Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

    Insert

    SpCas9 EQR
    

    Tags

    3xFLAG-NLS and NLS

    Expression

    Mammalian

    Mutation

    EQR (D1135E, R1335Q, and T1337R mutations in Cas9)

    Available Since

    Oct. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. px459 VRER

    Plasmid

    #101716

    Purpose

    sgRNA/SpCas9 expression plasmid with Cas9 VRER mutations (NGCG PAM)

    Depositor

    Andrew Holland

    Article

    Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

    Insert

    SpCas9 VRER
    

    Tags

    3xFLAG-NLS and NLS

    Expression

    Mammalian

    Mutation

    VRER (D1135V, G1218R, R1335E and T1337R)

    Available Since

    Oct. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. M-tdTom-SP

    Plasmid

    #48677

    Purpose

    Mammalian tdTomato activation reporter for SP with GTCCCCTCCACCCCACAGTG protospacer

    Depositor

    George Church

    Article

    Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

    Insert

    TAL binding site w/ GTCCCCTCCACCCCACAGTG protospacer, SP-PAM, and tdTomato reporter. Compatible with S. pyogenes Cas9
    

    Use

    CRISPR

    Promoter

    Sp6

    Available Since

    Oct. 22, 2013

    Availability

    Academic Institutions and Nonprofits only

15. px330 VRER

    Plasmid

    #101729

    Purpose

    Expresses a sgRNA and a Cas9 VRER variant that recognizes "NGCG" PAM motifs

    Depositor

    Andrew Holland

    Article

    Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

    Insert

    SpCas9 VRER
    

    Tags

    3xFLAG-NLS and NLS

    Expression

    Mammalian

    Mutation

    VRER (D1135V, G1218R, R1335E, and T1337R mutation…

    Available Since

    Oct. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLentiCRISPRv2 sgSp1

    Plasmid

    #174907

    Purpose

    Second generation sgRNA against Sp1

    Depositor

    Jane Clifford

    Article

    Swift et al Cell Rep. 2021 Mar 16;34(11):108840. doi

    Insert

    Specificity Protein 1 (SP1 Human)
    

    Expression

    Mammalian

    Mutation

    Missense mutation to mutate PAM sequence

    Promoter

    EFS-NS

    Available Since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. p458 VRER

    Plasmid

    #101728

    Purpose

    Expresses a sgRNA and a Cas9 VRER variant that recognizes "NGCG" PAM motifs

    Depositor

    Andrew Holland

    Article

    Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

    Insert

    SpCas9 VRER
    

    Tags

    3xFLAG-NLS and NLS

    Expression

    Mammalian

    Mutation

    VRER (D1135V, G1218R, R1335E, and T1337R mutation…

    Available Since

    Oct. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. p330 VQR

    Plasmid

    #101730

    Purpose

    Expresses a sgRNA and a Cas9 VQR variant that recognizes "NGA" PAM motifs

    Depositor

    Andrew Holland

    Article

    Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

    Insert

    SpCas9 VQR
    

    Tags

    3xFLAG-NLS and NLS

    Expression

    Mammalian

    Mutation

    VQR (D1135V, R1335Q, and T1337R mutations in Cas9)

    Available Since

    Oct. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. M-tdTom-NM

    Plasmid

    #48679

    Purpose

    Mammalian tdTomato activation reporter for NM with GTCCCCTCCACCCCACAGTG protospacer

    Depositor

    George Church

    Article

    Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

    Insert

    TAL binding site w/ GTCCCCTCCACCCCACAGTG protospacer, NM-PAM, and tdTomato reporter. Compatible with N. meningitidis Cas9
    

    Use

    CRISPR

    Promoter

    Sp6

    Available Since

    Oct. 22, 2013

    Availability

    Academic Institutions and Nonprofits only

20. pJEC581

    Plasmid

    #174369

    Purpose

    RFP reporter used to evaluate CRISPRa in bacteria, contains gRNA target sites every 10 bp upstream of the promoter.

    Depositor

    James Chappell

    Article

    Villegas Kcam et al Nucleic Acids Res. 2021 May 7;49(8):4793

    Insert

    RFP with PAM rich sequence upstream promoter
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    J23117

    Available Since

    Oct. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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