We narrowed to 31,666 results for: PLE

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pLEX307_CR-PDHA2

Plasmid

#115199

Purpose

Lentiviral transduction and expression of a CRISPR/Cas9-resistant PDHA2 into any mammalian cell (when using sgRNA 5'-ATGTAATGACGTGATCCGAG-3')

Depositor

Mohan Babu

Article

Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

Insert

CR (CRISPR/Cas9-resistant)-PDHA2WT (PDHA2 Human)

Use

Lentiviral

Mutation

c.321C>T (silent when translated to protein, c…

Promoter

EF1alpha

Available Since

March 26, 2021

Availability

Academic Institutions and Nonprofits only

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pLEX307_CR-PDHA2S291A

Plasmid

#115203

Purpose

Lentiviral transduction and expression of CRISPR/Cas9-resistant PDHA2S291A into any mammalian cell (when using sgRNA 5'-ATGTAATGACGTGATCCGAG-3')

Depositor

Mohan Babu

Article

Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

Insert

CR (CRISPR/Cas9-resistant)-PDHA2S291A (PDHA2 Human)

Use

Lentiviral

Mutation

c.321C>T (silent when translated to protein, c…

Promoter

EF1alpha

Available Since

March 26, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-sgSLC35B2

Plasmid

#83932

Purpose

Lentiviral vector expressing an sgRNA targeting SLC35B2 NOTE: This plasmid does NOT contain Cas9.

Depositor

David Sabatini

Article

Park et al Nat Genet. 2016 Dec 19. doi: 10.1038/ng.

Insert

sgSLC35B2

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-sgTPST2

Plasmid

#83933

Purpose

Lentiviral vector expressing an sgRNA targeting TPST2 NOTE: This plasmid does NOT contain Cas9.

Depositor

David Sabatini

Article

Park et al Nat Genet. 2016 Dec 19. doi: 10.1038/ng.

Insert

sgTPST2

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-sgCTRL-2

Plasmid

#83935

Purpose

Lentiviral vector expressing a control sgRNA. NOTE: This plasmid does NOT contain Cas9.

Depositor

David Sabatini

Article

Park et al Nat Genet. 2016 Dec 19. doi: 10.1038/ng.

Insert

sgCTRL-2

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-sgCTRL-3

Plasmid

#83936

Purpose

Lentiviral vector expressing a control sgRNA. NOTE: This plasmid does NOT contain Cas9.

Depositor

David Sabatini

Article

Park et al Nat Genet. 2016 Dec 19. doi: 10.1038/ng.

Insert

sgCTRL-3

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR-v1-sgNADK_1

Plasmid

#163462

Purpose

lentiviral vector expressing Cas9 and an sgRNA targeting NADK

Depositor

Jason Cantor

Article

Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

Insert

sgRNA 1 targeting NADK (NADK Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pLENTI_Hu_ECT2(T327D)

Plasmid

#136332

Purpose

Lentiviral expression of human ECT2_T327D

Depositor

Guillermo de Cárcer, Marcos Malumbres

Article

de Carcer et al Nat Med. 2017 Aug;23(8):964-974. doi: 10

Insert

ECT2 (ECT2 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

T327D

Available Since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv1-CENPB

Plasmid

#120208

Purpose

gRNA targeting the stem loop of CENPB poly(A) site

Depositor

David Bartel

Article

Wu et al Cell. 2017 May 18;169(5):905-917.e11. do

Insert

CENPB poly(A) site

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti6.3/NS4A-2K

Plasmid

#120838

Purpose

Lentiviral expression of ZIKV protein

Depositor

Jan Rehwinkel

Insert

NS4A-2K ZIKV

Use

Lentiviral

Tags

V5

Promoter

CMV

Available Since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti6.3/2K-NS4B

Plasmid

#120845

Purpose

Lentiviral expression of ZIKV protein

Depositor

Jan Rehwinkel

Insert

2K-NS4B ZIKV

Use

Lentiviral

Tags

V5

Promoter

CMV

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pSuper-PlexinB2

Plasmid

#115175

Purpose

shRNA against rodent PlexinB2

Depositor

Suzanne Paradis

Article

McDermott et al Mol Cell Neurosci. 2018 Jul 4;92:50-66.

Insert

shRNA targeting Plxnb2

Use

RNAi

Available Since

Sept. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR sgAMDHD1

Plasmid

#102316

Purpose

genetic depletion of AMDHD1

Depositor

David Sabatini

Article

Kanarek et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

sgAMDHD1 (AMDHD1 Human)

Use

Lentiviral

Promoter

U6

Available Since

July 13, 2018

Availability

Academic Institutions and Nonprofits only

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pLENTICRISPR sgNFS1

Plasmid

#102979

Purpose

Cutting NFS1 genomic locus

Depositor

Richard Possemato

Article

Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

Insert

sgNFS1 (NFS1 Human)

Use

CRISPR and Lentiviral

Available Since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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pLentiN MmuPV1 E6

Plasmid

#87661

Purpose

Lentiviral expression of MmuPV1 E6

Depositor

Karl Munger

Article

Meyers et al PLoS Pathog. 2017 Jan 20;13(1):e1006171.

Insert

MmuPV1 E6

Use

Lentiviral

Tags

Flag and HA

Promoter

CMV

Available Since

March 8, 2017

Availability

Academic Institutions and Nonprofits only

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pLEX_307-SOD1E133K

Plasmid

#83447

Purpose

Mammalian expression of SOD1E133K

Depositor

Mohan Babu

Article

Malty et al Cell Syst. 2017 Nov 7. pii: S2405-4712(1

Insert

SOD1 (SOD1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

E133K

Available Since

Nov. 9, 2016

Availability

Academic Institutions and Nonprofits only

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Plekhm1 pFastHFTb

Plasmid

#73827

Purpose

Express human Plekhm1 in insect cells

Depositor

Paul Odgren

Insert

Plekhm1 (PLEKHM1 Human)

Tags

6xHis and Flag

Expression

Insect

Available Since

March 15, 2016

Availability

Academic Institutions and Nonprofits only
pLexAOp (GB0118)

Plasmid

#68181

Purpose

Provides the 4xLexA Operator as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

4xOpLexA

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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Triple mutant

Plasmid

#63719

Purpose

Voltage sensing domain of Ciona with the A154D/R217Q/R229I mutations fused to super ecliptic pHlorin A227D

Depositor

Bradley Baker

Article

Piao et al J Neurosci. 2015 Jan 7;35(1):372-85. doi

Insert

Triple mutant

Tags

Super ecliptic pHlorin A227D

Expression

Mammalian

Promoter

CMV

Available Since

July 27, 2015

Availability

Academic Institutions and Nonprofits only
Gal4pBS13-Pleum

Plasmid

#60332

Purpose

Contains a promoter of two gal binding sites coupled with a core promoter derived from native Leucine promoter.

Depositor

Hal Alper

Article

Blazeck et al Biotechnol Bioeng. 2012 Nov;109(11):2884

Insert

promoter

Use

Synthetic Biology; Yeast synthetic hybrid promote…

Expression

Yeast

Available Since

May 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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