Skip to main content
Addgene
Showing: 801 - 820 of 63425 results

  1. PB_TRE_P9-PDGFR_EF1a_mPlum

    Plasmid
    #186307
    Purpose
    Plasmid for inducible mammalian cell expression of the P9 helixCAM and constitutive expression of an identifying fluorescent protein (mPlum). Flanked with PiggyBac sites for easy line construction.
    Depositor
    George Church
    Article
    Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01
    Inserts
    P9-PDGFR
    mPlum
    Use
    Tags
    HA, myc
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    186307-plasmid-map-sequence-id-366942

    Enlarge

  2. PB_TRE_AP10-PDGFR_EF1a_mOrange

    Plasmid
    #186308
    Purpose
    Plasmid for inducible mammalian cell expression of the AP10 helixCAM and constitutive expression of an identifying fluorescent protein (mOrange). Flanked with PiggyBac sites for easy line construction
    Depositor
    George Church
    Article
    Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01
    Inserts
    AP10-PDGFR
    mOrange
    Use
    Tags
    HA, myc
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    186308-plasmid-map-sequence-id-366950

    Enlarge

  3. PB_TRE_P3-PDGFR_EF1a_iRFP670

    Plasmid
    #186305
    Purpose
    Plasmid for inducible mammalian cell expression of the P3 helixCAM and constitutive expression of an identifying fluorescent protein (iRFP670). Flanked with PiggyBac sites for easy line construction.
    Depositor
    George Church
    Article
    Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01
    Inserts
    P3-PDGFR
    iRFP670
    Use
    Tags
    HA, myc
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    186305-plasmid-map-sequence-id-366925

    Enlarge

  6. pDUAL_tacI

    Plasmid
    #155170
    Purpose
    BaSP and CuCBP co-expression: tacI promoter controls BaSP expression
    Depositor
    Bernd Nidetzky
    Article
    Schwaiger et al Biotechnol J. 2020 Jul 15:e2000063. doi:
    Inserts
    Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
    Cellobiose phosphorylase from Cellulomonas uda
    Use
    Synthetic Biology
    Tags
    His Tag and Strep - Tag II
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    155170-plasmid-map-sequence-id-311666

    Enlarge

  7. EF1a_mCherry_P2A_Hygro

    Plasmid
    #135003
    Purpose
    Lentiviral vector to express mCherry or to clone and express gene of interest, under control of EF1a promoter. Barcodes compatible with detection in single cell RNA-seq may be inserted at HpaI site.
    Depositor
    Prashant Mali
    Article
    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Feb. 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    135003-plasmid-map-sequence-id-272070

    Enlarge

  9. pDUAL_2_araBAD

    Plasmid
    #155176
    Purpose
    see pDUAL_araBAD: exchanged origin of replication (pBR322) and introduced bom site
    Depositor
    Bernd Nidetzky
    Article
    Schwaiger et al Biotechnol J. 2020 Jul 15:e2000063. doi:
    Inserts
    Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
    Cellobiose phosphorylase from Cellulomonas uda
    Use
    Synthetic Biology
    Tags
    His Tag and Strep - Tag II
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    155176-plasmid-map-sequence-id-310563

    Enlarge

  10. pSCMV-H2B-FT-Medium

    Plasmid
    #157670
    Purpose
    Retroviral vector for expressing chimeric human histone H2B fused with the "Medium" variant of the monomeric color-changing fluorescent timer (FT) in mammalian cells.
    Depositor
    Shangqin Guo
    Article
    Eastman et al Cell Rep. 2020 Jun 23;31(12):107804. doi
    Insert
    Histone H2B
    Use
    Retroviral
    Tags
    Medium-FT
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    157670-plasmid-map-sequence-id-308113

    Enlarge

  11. pECIA2

    Plasmid
    #47032
    Purpose
    This is a Gateway destination vector for secreted expression in Drosophila cell culture by induction via CuSO4. Inserts will be C-terminally tagged with Fc, V5 and hexahistidine tags.
    Depositor
    Chris Garcia
    Article
    Ozkan et al Cell. 2013 Jul 3;154(1):228-39. doi: 10.
    Type
    Empty backbone
    Use
    Secreted expression in drosophila culture
    Tags
    Fc(human IgG1), HRV 3C Protease Cleavage site, He…
    Expression
    Insect
    Mutation
    Promoter
    Metallothionein (Copper-Inducible)
    Available since
    Aug. 30, 2013
    Availability
    Academic Institutions and Nonprofits only
    47032-plasmid-map-sequence-id-67069

    Enlarge

  12. pSCMV-H2B-FT-Slow

    Plasmid
    #157671
    Purpose
    Retroviral vector for expressing chimeric human histone H2B fused with the "Slow" variant of the monomeric color-changing fluorescent timer (FT) in mammalian cells.
    Depositor
    Shangqin Guo
    Article
    Eastman et al Cell Rep. 2020 Jun 23;31(12):107804. doi
    Insert
    Histone H2B
    Use
    Retroviral
    Tags
    Slow-FT
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    157671-plasmid-map-sequence-id-311902

    Enlarge

  13. pSCMV-H2B-FT-Fast

    Plasmid
    #157669
    Purpose
    Retroviral vector for expressing chimeric human histone H2B fused with the "Fast" variant of the monomeric color-changing fluorescent timer (FT) in mammalian cells.
    Depositor
    Shangqin Guo
    Article
    Eastman et al Cell Rep. 2020 Jun 23;31(12):107804. doi
    Insert
    Histone H2B
    Use
    Retroviral
    Tags
    Fast-FT
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    157669-plasmid-map-sequence-id-308111

    Enlarge

  16. pBS SK mCherryROSAbsr

    Plasmid
    #54321
    Purpose
    Encodes mCherry fused with out-of-frame blasticidin-S resistance gene with a linker containing a target for TALEN/CRISPR. Can use as a surrogate target to select cells expressing active TALEN/CRISPR.
    Depositor
    Silvestro Conticello
    Article
    Niccheri et al Cell Mol Life Sci. 2017 Apr 18. doi: 10.
    Insert
    mCherry and BlasticidinS-resistance
    Use
    CRISPR, Mouse Targeting, and TALEN
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    54321-plasmid-map-sequence-id-84455

    Enlarge

  18. pBSR (mCherry-surrogateTarget-bsr)

    Plasmid
    #66950
    Purpose
    Encodes mCherry fused with out-of-frame blasticidin-S resistance gene with a linker containing a target for TALEN/CRISPR. Can use as a surrogate target to select cells expressing active TALEN/CRISPR.
    Depositor
    Silvestro Conticello
    Article
    Niccheri et al Cell Mol Life Sci. 2017 Apr 18. doi: 10.
    Insert
    blasticidin
    Use
    CRISPR, Mouse Targeting, and TALEN
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    beta-actin
    Available since
    July 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    66950-plasmid-map-sequence-id-115273

    Enlarge

  19. pDUAL_2_T5

    Plasmid
    #155175
    Purpose
    see pDUAL_T5: exchanged origin of replication (pBR322) and introduced bom site
    Depositor
    Bernd Nidetzky
    Article
    Schwaiger et al Biotechnol J. 2020 Jul 15:e2000063. doi:
    Inserts
    Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
    Cellobiose phosphorylase from Cellulomonas uda
    Use
    Synthetic Biology
    Tags
    His Tag and Strep - Tag II
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    155175-plasmid-map-sequence-id-310562

    Enlarge

Showing: 801 - 820 of 63425 results