We narrowed to 901 results for: golden gate assembly
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Plasmid#239695PurposeBsaI Golden Gate assembly plasmid encoding the mRuby2 CDSDepositorInsertmRuby2
UseSynthetic BiologyAvailable SinceJuly 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKG2039_pPV1-CAG
Plasmid#239686PurposeBsaI Golden Gate assembly plasmid encoding the CAG promoterDepositorInsertCAG promoter
UseSynthetic BiologyAvailable SinceJuly 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pX330-BbsI-PITCh
Plasmid#127875PurposeFor user to clone their gRNA oligos to BbsI site. PITCh gRNA expression cassette is already build in so there is no need to perform Golden Gate assemblyDepositorTypeEmpty backboneUseCRISPRExpressionMammalianAvailable SinceJuly 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCEC-red
Plasmid#196040PurposeAllows the expression of Cas9 in Saccharomyces cerevisiae and contains a gRNA expression unit with a mRFP1 placeholder for user-defined gRNA insertion using BsaI-mediated Golden Gate AssemblyDepositorTypeEmpty backboneUseCRISPR and Synthetic Biology; Grna cloningExpressionYeastAvailable SinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHelper_ShCAST_sgRNA
Plasmid#127921PurposeExpresses ShCAST and an empty sgRNA scaffold. New targets can be added using Golden Gate assembly (LguI sites).DepositorInsertShTnsB, ShTnsC, ShTniQ, ShCas12k, sgRNA scaffold for guide cloning (LguI sites)
Available SinceJuly 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
Lenti-entry-puro
Plasmid#85745PurposeLentiviral vector for generating multiple sgRNAs carrying plasmids by Golden Gate Assembly.DepositorTypeEmpty backboneUseLentiviralAvailable SinceJan. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pShHELIX_sgRNA_entry (CJT32)
Plasmid#181782PurposeExpresses ShHELIX containing a nicking I-AniI fusion to ShTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).DepositorInsertnAniI-ShTnsB, ShTnsC, ShTniQ, ShCas12k
ExpressionBacterialMutationnAniI = K227M, F80K, L232KPromoterLac and J23119Available SinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAcHELIX-sgRNA_entry (CJT94)
Plasmid#181783PurposeExpresses AcHELIX containing a nicking I-AniI fusion to AcTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).DepositorInsertnAniI-AcTnsB, AcTnsC, AcTniQ, AcCas12k
ExpressionBacterialMutationnAniI = K227M, F80K, L232KPromoterLac and J23119Available SinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pShoHELIX-sgRNA_entry (BO3)
Plasmid#181784PurposeExpresses ShoHELIX containing a nicking I-AniI fusion to ShoTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).DepositorInsertnAniI-ShoTnsB, ShoTnsC, ShoTniQ, ShoCas12k
ExpressionBacterialMutationnAniI = K227M, F80K, L232KPromoterLac and J23119Available SinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKG2197_pPV2-ZF10-NS3-p65
Plasmid#239700PurposeBsaI Golden Gate assembly plasmid encoding the SynZiFTR ZF10-NS3-p65 CDSDepositorInsertZF10-NS3-p65
UseSynthetic BiologyAvailable SinceSept. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKG1313_pPV3-WPRE
Plasmid#239703PurposeBsaI Golden Gate assembly plasmid encoding the WPRE 3' UTR sequenceDepositorInsertWPRE 3' UTR
UseSynthetic BiologyAvailable SinceJuly 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKG3560_pHarbor-Rogi2_backbone
Plasmid#239706PurposePaqCI Golden Gate assembly plasmid encoding the backbone for Bxb1-mediated integration in the Rogi2 landing pad lineDepositorTypeEmpty backboneUseSynthetic BiologyAvailable SinceJuly 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKG1367_pPV1-hPGK
Plasmid#239691PurposeBsaI Golden Gate assembly plasmid encoding the hPGK promoterDepositorInserthPGK promoter
UseSynthetic BiologyMutationRemoval of BsaI cut sitesAvailable SinceJuly 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKG0743_pPV1-ZF43x6-C
Plasmid#239693PurposeBsaI Golden Gate assembly plasmid encoding the COMET ZF43x6-C promoterDepositorInsertZF43x6-C promoter
UseSynthetic BiologyAvailable SinceJuly 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKG3657_pPV1-ZF10.BSx8
Plasmid#239694PurposeBsaI Golden Gate assembly plasmid encoding the synZiFTR ZF10.BSx8 promoterDepositorInsertZF10.BSx8 promoter
UseSynthetic BiologyAvailable SinceJuly 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKG1055_pPV2-tagBFP
Plasmid#239697PurposeBsaI Golden Gate assembly plasmid encoding the tagBFP CDSDepositorInserttagBFP
UseSynthetic BiologyMutationRemoval of BsaI cut sitesAvailable SinceJuly 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKG3659_pPV2-rtTA-P2A-iRFP720
Plasmid#239698PurposeBsaI Golden Gate assembly plasmid encoding the rtTA-P2A-iRFP720 CDSDepositorInsertrtTA-P2A-iRFP720
UseSynthetic BiologyAvailable SinceJuly 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKG0587_pPV3-bGH
Plasmid#239701PurposeBsaI Golden Gate assembly plasmid encoding the bGH PASDepositorInsertbGH PAS
UseSynthetic BiologyAvailable SinceJuly 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKG0588_pPV3-SV40
Plasmid#239702PurposeBsaI Golden Gate assembly plasmid encoding SV40 PASDepositorInsertSV40 PAS
UseSynthetic BiologyAvailable SinceJuly 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKG2019_pPV1-EF1a
Plasmid#239687PurposeBsaI Golden Gate assembly plasmid encoding the EF1a promoterDepositorInsertEF1a promoter
UseSynthetic BiologyAvailable SinceJuly 10, 2025AvailabilityAcademic Institutions and Nonprofits only