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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,063 results for: golden gate assembly

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Showing: 801 - 820 of 1063 results

  3. pJB55

    Plasmid
    #226310
    Purpose
    E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.
    Depositor
    David Savage
    Article
    Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d
    Insert
    CsoS2 with V(T/S)G to AAA CsoS2 MR1-5
    Expression
    Bacterial
    Mutation
    VTG273-5AAA, VTG284-6AAA, VTG295-7AAA, VSG332-4AA…
    Promoter
    pTRC
    Available Since
    Oct. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    226310-plasmid-map-sequence-id-449922

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  4. pKL2-138

    Plasmid
    #219413
    Purpose
    Estradiol-inducible expression of 42-10 Zinc finger activator
    Depositor
    Ahmad Khalil
    Article
    Bragdon et al Cell. 2023 Aug 31;186(18):3810-3825.e18.
    Insert
    {pTEF1-ZEV}{pGal1(Zif268op)x6-3xFLAG-NLS-VP16-mRuby2-(42-10-4x)-PDZLigWT-tSSA1}
    Expression
    Yeast
    Mutation
    Four Arg to Ala mutations in zinc finger backbone
    Available Since
    May 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    219413-plasmid-map-sequence-id-434474

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  7. pJB50

    Plasmid
    #226311
    Purpose
    E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.
    Depositor
    David Savage
    Article
    Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d
    Insert
    CsoS2 with V(T/S)G to AAA CsoS2 MR1-7
    Expression
    Bacterial
    Mutation
    VTG273-5AAA, VTG284-6AAA, VTG295-7AAA, VSG332-4AA…
    Promoter
    pTRC
    Available Since
    Oct. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    226311-plasmid-map-sequence-id-449923

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  9. pYL001

    Plasmid
    #249611
    Purpose
    Cloning vector for PaqCI Golden Gate assembly of ISYmu1 gRNAs into TRV2 with mutation SlFT element
    Depositor
    Steven Jacobsen
    Article
    Liu et al bioRxiv 2025.11.08.687402
    Type
    Empty backbone
    Expression
    Plant
    Available Since
    Jan. 12, 2026
    Availability
    Academic Institutions and Nonprofits only
    249611-plasmid-map-sequence-id-496977

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Showing: 801 - 820 of 1063 results