We narrowed to 14,531 results for: pcDNA3

1. HA-USPL1-pcDNA3.1

   Plasmid

   #85760

   Purpose

   mammalian expression of full length HA tagged USPL1

   Depositor

   Frauke Melchior

   Article

   Schulz et al EMBO Rep. 2012 Oct;13(10):930-8. doi: 10

   Insert

   USPL1, full length (USPL1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.3_SARS2_XBB.1.5

   Plasmid

   #196585

   Purpose

   expressing SARS-CoV-2 XBB.1.5 spike protein for pseudovirus production

   Depositor

   David Nemazee

   Insert

   Spike (XBB.1.5)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   G252V, F486P on the basis of XBB

   Promoter

   CMV

   Available since

   March 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCDNA3_Sec61B-V5-APEX2

   Plasmid

   #83411

   Purpose

   Expressed C-term tagged APEX2 on Sec61B in mammalian cell

   Depositor

   Hyun-Woo Rhee

   Article

   Lee et al Cell Rep. 2016 May 11. pii: S2211-1247(1

   Insert

   Sec61B-V5-APEX2 (SEC61B Synthetic, Human)
   

   Use

   Tags

   APEX2 and V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-F

   Available since

   March 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDNA3- SREBP-1

   Plasmid

   #204192

   Purpose

   Mammalian expression of SREBP-1 (isoform 1)

   Depositor

   Allan Weissman

   Article

   Yang et al J Biol Chem. 2023 Oct 12;299(12):105346.

   Insert

   SREBP-1 (SREBF1 Human)
   

   Use

   Tags

   E-tag and T7x2

   Expression

   Mammalian

   Mutation

   multiple silent mutations

   Promoter

   Available since

   April 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-HA-CCND3

   Plasmid

   #172621

   Purpose

   Expresses HA-tagged cyclin D3 in mammalian cells

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Insert

   CCND3 (CCND3 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1(+)-blueAKAR

   Plasmid

   #118470

   Purpose

   cpBFP-based single-color biosensor for monitoring Protein Kinase A activity.

   Depositor

   Jin Zhang

   Article

   Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

   Insert

   blueAKAR
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3.1-Beta1

   Plasmid

   #140987

   Purpose

   Encodes a Gbeta subunit (GNB1) as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

   Depositor

   Bryan Roth

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Insert

   GBeta1 (GNB1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Contains an N-terminal human rhinovirus (HRV) 3C …

   Promoter

   CMV

   Available since

   June 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1-mPCSK3

   Plasmid

   #122674

   Purpose

   Expresses mouse PCSK3 in mammalian cells

   Depositor

   Liming Pei

   Article

   Li et al Mol Cell Biol. 2018 Oct 15;38(21). pii:

   Insert

   Mus musculus furin (paired basic amino acid cleaving enzyme) (Furin), transcript variant 1 (Furin Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   T7

   Available since

   May 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-sapphireCKAR(T/A)

   Plasmid

   #118469

   Purpose

   Negative-control mutant for sapphireCKAR biosensor.

   Depositor

   Jin Zhang

   Article

   Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

   Insert

   sapphireCKAR(T/A)
   

   Use

   Tags

   6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

   Expression

   Mammalian

   Mutation

   Contains Thr-to-Ala mutation in substrate sequenc…

   Promoter

   CMV

   Available since

   Dec. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCDNA3.3_CoV2_B.1.1.7

    Plasmid

    #170451

    Purpose

    Express SARS-CoV-2 B.1.1.7 (UK strain / alpha strain) spike protein with an 18aa deletion on the C-terminal tail. Used for SARS2 (UK strain) pseudotyped virus production.

    Depositor

    David Nemazee

    Article

    Yuan et al Science. 2021 Aug 13;373(6556):818-823.

    Insert

    SARS-CoV-2 501V2 spike D18 (S SARS-CoV-2, UK strain (B.1.1.7))
    

    Use

    Tags

    NA

    Expression

    Mammalian

    Mutation

    HY69-70del, Y144del, N501Y, A570D, D614G, P681H, …

    Promoter

    CMV

    Available since

    May 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. T7-Elongin C-pcDNA3

    Plasmid

    #19998

    Depositor

    William Kaelin

    Article

    Lonergan et al Mol Cell Biol. 1998 Feb . 18(2):732-41.

    Insert

    Elongin C (ELOC Human)
    

    Use

    Tags

    T7-tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 6, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1(+)-blueAKAR(T/A)

    Plasmid

    #118471

    Purpose

    Negative-control mutant for blueAKAR biosensor.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    blueAKAR(T/A)
    

    Use

    Tags

    6xHis, T7 tag (gene 10 leader), and Xpress (TM) t…

    Expression

    Mammalian

    Mutation

    Contains Thr-to-Ala mutation in substrate sequenc…

    Promoter

    CMV

    Available since

    April 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.2 YFP

    Plasmid

    #84910

    Purpose

    expression of YFP in mammalian cells

    Depositor

    Aaron Gitler

    Article

    Elden et al Nature. 2010 Aug 26;466(7310):1069-75. d

    Insert

    YFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCDNA3_Cas7-11_T2A_Csx29

    Plasmid

    #197607

    Purpose

    Expresses Cas7-11 and Csx29 in mammalian cells

    Depositor

    Feng Zhang

    Article

    Strecker et al Science. 2022 Nov 25;378(6622):874-881.

    Insert

    Cas7-11_T2A_Csx29
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. miR155 in pcDNA3.1

    Plasmid

    #78126

    Purpose

    Mammalian expression of miR155

    Depositor

    Heidi Schwarzenbach

    Article

    Eichelser et al Oncotarget. 2014 Oct 30;5(20):9650-63.

    Insert

    miR155 (MIR155 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3-V5-DGCR8

    Plasmid

    #51383

    Depositor

    Narry Kim

    Article

    Yeom et al Nucleic Acids Res. 2006;34(16):4622-9. E

    Insert

    DGCR8 (DGCR8 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3.1-mhYFP

    Plasmid

    #186533

    Purpose

    Monomeric hyperfolder YFP fluorescent protein: cytosolic expression in mammalian cells

    Depositor

    Gregory Petsko

    Article

    Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

    Insert

    Monomeric hyperfolder YFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    hfYFP-S147P/L195M/V206K

    Promoter

    CMV

    Available since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. Pyronic/pcDNA3.1(-)

    Plasmid

    #51308

    Purpose

    Pyronic, FRET sensor that allow quantitative measurement of pyruvate transport, pyruvate production and mitochondrial pyruvate consumption in intact individual cells at high temporal resolution.

    Depositor

    Luis Felipe Barros

    Article

    San Martin et al PLoS One. 2014 Jan 21;9(1):e85780. doi:

    Insert

    Pyronic
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Keratin 8 (pcDNA3)

    Plasmid

    #18063

    Depositor

    M Bishr Omary

    Article

    Ku et al J Biol Chem. 2002 Mar 29. 277(13):10775-

    Insert

    Keratin 8 (KRT8 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 16, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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20. GZnP3 in pcDNA3.1+

    Plasmid

    #161738

    Purpose

    Cytosolic fluorescent reporter for Zn²⁺ (Kd = 1.3 nM, ex/em = 488 nm/515 nm)

    Depositor

    Yan Qin

    Article

    Minckley et al Nat Commun. 2019 Oct 22;10(1):4806. doi:

    Insert

    GZnP3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge