166,542 results

1. hSTARR-seq_SCP1 vector_blocking 4

   Plasmid

   #99319

   Purpose

   Vector to measure enhancer activity of candidates from a DNA library by STARR-seq.

   Depositor

   Alexander Stark

   Article

   Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

   Type

   Empty backbone

   Use

   Hstarr-seq screening vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. dimericTomato-2xrGBD

   Plasmid

   #176098

   Purpose

   Visualization of active RhoA

   Depositor

   Dorus Gadella

   Article

   Mahlandt et al J Cell Sci. 2021 Aug 6. pii: 271216. doi

   Insert

   rhotekin (Rtkn Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   CMVdel

   Available Since

   Oct. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pIRE4-Azi

   Plasmid

   #105829

   Purpose

   Plasmid encoding for the translational pair that enables efficent incoporation of p-Azido-phenylalanine in response to the amber codon into proteins in mammmalian cells

   Depositor

   Irene Coin

   Article

   Seidel et al Elife. 2017 Aug 3;6. doi: 10.7554/eLife.

   Inserts

   Humanized EAziRS
   4 copies of the U6-BstYam tRNA cassette
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   (Y37L, D182S, F183M, L186A, D265R)TyrRS

   Promoter

   PGK and U6

   Available Since

   March 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3 HA Arf1 DN T31N

   Plasmid

   #10833

   Depositor

   Thomas Roberts

   Article

   Furman et al J Biol Chem. 2002 Mar 8. 277(10):7962-9.

   Insert

   Arf1 T31N (ARF1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   T31N. Defective in GTP binding. Dominant negati…

   Promoter

   Available Since

   April 20, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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5. negMaLionB

   Plasmid

   #113911

   Purpose

   negative control for Blue ATP sensor

   Depositor

   Tetsuya Kitaguchi

   Article

   Arai et al Angew Chem Int Ed Engl. 2018 Jun 27. doi

   Insert

   negMaLionB
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. LncRNA overexpression EF1α_BbsI_SV40 polyA_EGFP

   Plasmid

   #219828

   Purpose

   Overexpression vector for non-coding RNAs driven by EF1α promoter with GFP. Cloning using BbsI.

   Depositor

   Philipp Maass

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   July 31, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. HA GSK3 beta wt pcDNA3

   Plasmid

   #14753

   Purpose

   Mammalian expression of human GSK3 beta with HA tag

   Depositor

   Jim Woodgett

   Article

   He et al Nature. 1995 Apr 13. 374(6523):617-22.

   Insert

   GSK3 beta (GSK3B Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 12, 2007

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. LentiV_Neo_LKB1

   Plasmid

   #108111

   Purpose

   Lentiviral expression plasmid of human LKB1 cDNA with neomycin resistance gene

   Depositor

   Christopher Vakoc

   Article

   Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

   Insert

   LKB1 (STK11 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   EFS promoter

   Available Since

   April 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. BB3cH_pGAP_23*_pLAT1_Cas9

   Plasmid

   #104907

   Purpose

   hCas9 under control of LAT1 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on Hyg

   Depositor

   Brigitte Gasser

   Article

   Gassler et al Methods Mol Biol. 2019;1923:211-225. doi

   Inserts

   HH - FS23 linker - HDV
   human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Jan. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBI-MCS-EGFP

    Plasmid

    #16542

    Depositor

    Bert Vogelstein

    Article

    Yu et al Proc Natl Acad Sci U S A. 1999 Dec 7. 96

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 11, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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