We narrowed to 21,351 results for: Mut

1. pcDNA3-HA-DNM2-W525L

   Plasmid

   #155263

   Purpose

   Mammalian expression of rat DNM2 fused to HA

   Depositor

   David Rubinsztein

   Article

   Puri et al Dev Cell. 2020 Apr 20;53(2):154-168.e6.

   Insert

   dynamin 2 (Dnm2 Rat)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Tryptophan 525 to Leucine

   Promoter

   Available Since

   June 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA-Flag-EcoCas6

   Plasmid

   #106274

   Purpose

   Encodes E.coli CRISPR Type I-E CasE with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter

   Depositor

   Charles Gersbach

   Article

   Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

   Insert

   EcoCasE
   

   Use

   CRISPR

   Tags

   Flag, NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pIKAT111

   Plasmid

   #69996

   Purpose

   Mammalian expression of cytochrome p450 CYP2B6 variant

   Depositor

   Kathrin Klein

   Article

   Radloff et al Hum Mutat. 2013 May;34(5):725-34. doi: 1

   Insert

   CYP2B6 (CYP2B6 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   C1459T (R487C)

   Promoter

   CMV

   Available Since

   Nov. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRR.6

   Plasmid

   #69997

   Purpose

   Mammalian expression of cytochrome p450 CYP2B6 variant

   Depositor

   Kathrin Klein

   Article

   Radloff et al Hum Mutat. 2013 May;34(5):725-34. doi: 1

   Insert

   CYP2B6 (CYP2B6 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Q172H, K262R

   Promoter

   CMV

   Available Since

   Nov. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAc5.1B-EGFP-DmNot1_J

   Plasmid

   #146709

   Purpose

   Insect Expression of DmNot1

   Depositor

   Elisa Izaurralde

   Article

   Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

   Insert

   DmNot1 (Not1 Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Three non silent mutations N605S, K759M, G1999S a…

   Promoter

   Available Since

   May 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. CRY-tTA (B702)

   Plasmid

   #92032

   Purpose

   Expresses CRY2 (Full length, mutated NLS) fusion with tTA2 'tet-OFF' transcriptional activator

   Depositor

   Chandra Tucker

   Article

   Pathak et al Nucleic Acids Res. 2017 Apr 20. doi: 10.

   Insert

   CRY2 (CRY2 Mustard Weed)
   

   Use

   Tags

   tTA2 (TetR fused to VP64)

   Expression

   Mammalian

   Mutation

   NLS on CRY2 is mutated

   Promoter

   CMV

   Available Since

   July 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDual-eGFP(W66)

   Plasmid

   #63217

   Purpose

   Expression of the Celeste (cyan) spectral variant of eGFP in bacteria and in mammalian cells. Could be used as a reporter for quantifying gene targeting and recombineering in mammalian cells.

   Depositor

   Richard S Myers

   Article

   Valledor et al IUBMB Life. 2012 Aug;64(8):684-9. doi: 1

   Insert

   His-T7-eGFP(W66)
   

   Use

   Lentiviral

   Tags

   His6 and T7

   Expression

   Bacterial and Mammalian

   Mutation

   Changed tyrosine at position 66 with respect to t…

   Promoter

   CMV-EF1α hybrid (CEF)

   Available Since

   April 15, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pLenti-LmoCas6-KRAB-2a-BlastR

   Plasmid

   #126492

   Purpose

   Encodes lentivirus expression of L. monocytogenes CRISPR Type I-B Flag-Cas6-KRAB-2a-BlastR driven by hUbC promoter

   Depositor

   Charles Gersbach

   Article

   Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

   Insert

   LmoCas6-KRAB-2a-BlastR
   

   Use

   CRISPR and Lentiviral

   Tags

   Flag, NLS

   Expression

   Mammalian

   Mutation

   Promoter

   hUbC

   Available Since

   Sept. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA4/HisMaxB-YAP1-S61A

   Plasmid

   #18979

   Depositor

   Marius Sudol

   Article

   Oka et al J Biol Chem. 2008 Oct 10;283(41):27534-2

   Insert

   Yes-kinase associated protein S61A mutant (YAP1 Human)
   

   Use

   Tags

   His

   Expression

   Mammalian

   Mutation

   Serine 61 changed to Alanine

   Promoter

   Available Since

   Sept. 8, 2008

   Availability

   Academic Institutions and Nonprofits only

10. pEGFP-PH-SWAP70(R223E, R224E)

    Plasmid

    #84583

    Purpose

    Expresses GFP-tagged PH-domain of SWAP70 in mammalian cells, impaired phosphoinositide binding

    Depositor

    Geert van den Bogaart

    Article

    Baranov et al Cell Rep. 2016 Nov 1;17(6):1518-1531. do

    Insert

    SWAP70 residues 209-306 with mutations R223E and R224E (SWAP70 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Pleckstrin-homology domain, residues 209-306, mut…

    Promoter

    CMV

    Available Since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

11. YFP-synDAT-S/A

    Plasmid

    #48792

    Purpose

    Expresses YFP-tagged human DAT with first 5 N-terminal serines mutated to alanine in pCIHygro

    Depositor

    Jonathan Javitch

    Article

    Fog et al Neuron. 2006 Aug 17;51(4):417-29.

    Insert

    YFP-synhDAT-S/A (SLC6A3 Human)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Ser2, Ser4, Ser7, Ser12, and Ser13 mutated simult…

    Promoter

    Available Since

    Oct. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. YFP-synDAT-S/D

    Plasmid

    #48793

    Purpose

    Expresses YFP-tagged human DAT with first 5 N-terminal serines mutated to aspartates in pCIHygro

    Depositor

    Jonathan Javitch

    Article

    Fog et al Neuron. 2006 Aug 17;51(4):417-29.

    Insert

    YFP-synhDAT-S/D (SLC6A3 Human)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Ser2, Ser4, Ser7, Ser12, and Ser13 mutated simult…

    Promoter

    Available Since

    Oct. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA-Flag-EcoCas8e

    Plasmid

    #106270

    Purpose

    Encodes human codon optimized E.coli CRISPR Type I-E CasA with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter

    Depositor

    Charles Gersbach

    Article

    Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

    Insert

    EcoCasA
    

    Use

    CRISPR

    Tags

    Flag, NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA-Flag-EcoCse2

    Plasmid

    #106271

    Purpose

    Encodes human codon optimized E.coli CRISPR Type I-E CasB with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter

    Depositor

    Charles Gersbach

    Article

    Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

    Insert

    EcoCasB
    

    Use

    CRISPR

    Tags

    Flag, NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA-Flag-EcoCas7

    Plasmid

    #106272

    Purpose

    Encodes human codon optimized E.coli CRISPR Type I-E CasC with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter

    Depositor

    Charles Gersbach

    Article

    Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

    Insert

    EcoCasC
    

    Use

    CRISPR

    Tags

    Flag, NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA-Flag-EcoCas5

    Plasmid

    #106273

    Purpose

    Encodes human codon optimized E.coli CRISPR Type I-E CasD with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter

    Depositor

    Charles Gersbach

    Article

    Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

    Insert

    EcoCasD
    

    Use

    CRISPR

    Tags

    Flag, NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. C211

    Plasmid

    #174360

    Purpose

    For expressing siRNA-resistant human PABPC1 mutant (I110L, D111E, D117E, A121G, G139A, Y140F, T147S, R166K) in human cell lines via transfection

    Depositor

    David Bartel

    Article

    Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

    Insert

    PABPC1 (PABPC1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    I110L, D111E, D117E, A121G, G139A, Y140F, T147S, …

    Promoter

    Available Since

    Feb. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. RB-409-815-pGex2T

    Plasmid

    #21784

    Depositor

    William Kaelin

    Article

    Kaelin et al Cell. 1991 Feb 8. 64(3):521-32.

    Insert

    Rb (RB1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    deletion mutant ORF contains aa 409-815 of wt Rb …

    Promoter

    Available Since

    Aug. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBp-FGFR2c-W290C

    Plasmid

    #45701

    Depositor

    Matthew Meyerson

    Article

    Liao et al Cancer Res. 2013 Jun 20.

    Insert

    FGFR2 IIIc (FGFR2 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    W290C

    Promoter

    Available Since

    June 17, 2013

    Availability

    Academic Institutions and Nonprofits only

20. pBp-FGFR2b-P253R

    Plasmid

    #45716

    Depositor

    Matthew Meyerson

    Article

    Liao et al Cancer Res. 2013 Jun 20.

    Insert

    FGFR2 IIIb (FGFR2 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    P253R

    Promoter

    Available Since

    June 27, 2013

    Availability

    Academic Institutions and Nonprofits only