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pSC2+-HyPerFLEX-Lifeact

Plasmid

#217568

Purpose

Mammalian expression HyPerFLEX targeted to actin filaments

Depositor

Vsevolod Belousov

Article

Potekhina et al Nat Chem Biol. 2025 Oct 16. doi: 10.1038

Insert

HyPerFLEX-Lifeact

Expression

Mammalian

Promoter

CMV IE94

Available Since

Oct. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pET-NIC-His6-TEV-SHP2-PTP(T507K)

Plasmid

#245217

Purpose

Production of His-tagged SHP2 PTP domain

Depositor

Neel Shah

Article

van Vlimmeren et al bioRxiv [Preprint]. 2025 Jul 2:2025.06.3

Insert

SHP2 PTP domain

Tags

6xHis

Expression

Bacterial

Mutation

T507K

Available Since

Oct. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pCK883

Plasmid

#242919

Purpose

Constitutive expression of dCas9, MCP-SoxS-SYNZIP6, and αNTD-SYNZIP5

Depositor

Jesse Zalatan

Article

Kiattisewee et al ACS Synth Biol. 2025 Jul 22. doi: 10.102

Insert

MCP-SoxS-SYNZIP6

Use

CRISPR and Synthetic Biology

Promoter

BBa_J23107

Available Since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pKO024.306-107

Plasmid

#242927

Purpose

Expression of a Sth1-dCas9 compatible MS2 scRNA J306 and a Spy-dCas9 compatible sgRNA J107

Depositor

Jesse Zalatan

Article

Kiattisewee et al ACS Synth Biol. 2025 Jul 22. doi: 10.102

Insert

sgRNA J107

Use

CRISPR and Synthetic Biology

Promoter

Bba_J23119

Available Since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pET-28b His6-TEV-SHP2-FL(H114F)

Plasmid

#245204

Purpose

Production of His-tagged full-length SHP2

Depositor

Neel Shah

Article

Jiang et al Nat Commun. 2025 Jul 1;16(1):5464. doi:

Insert

SHP2 full-length

Tags

6xHis

Expression

Bacterial

Mutation

H114F

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pDonr 201_PHIP

Plasmid

#240328

Purpose

Entry vector for Gateway with PHIP

Depositor

Junjie Chen

Article

Nie et al Genes Dev. 2022 Sep 1;36(17-18):1016-103

Insert

PHIP (PHIP Human)

Expression

Bacterial

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pDonr201_TNKS

Plasmid

#240329

Purpose

Entry vector for Gateway with TNKS

Depositor

Junjie Chen

Article

Nie et al Mol Cell Proteomics. 2020 Dec;19(12):201

Insert

TNKS (TNKS Human)

Expression

Bacterial

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pJL191

Plasmid

#243690

Purpose

Stably expresses a chimeric protein, SUPV3L1(1-199aa)-ALDH18A1(106aa-385aa)-SUPV3L1(451-786aa)

Depositor

Jonathan Weissman

Article

Luo et al Cell. 2025 Aug 25:S0092-8674(25)00916-X.

Insert

SUPV3L1(1-199aa)-ALDH18A1(106aa-385aa)-SUPV3L1(451-786aa)

Use

Lentiviral

Promoter

EF1a

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiLox7.0-mVenus-CAGEn-Caax (Nrdj1)

Plasmid

#241405

Purpose

Proximity-triggered Protein Trans-Splicing between mVenus and RFP, Figure 1 in Nature Chemical Biology 20.10 (2024): 1353-1360.

Depositor

Thomas Muir

Article

Lee et al Nat Chem Biol. 2024 Oct;20(10):1353-1360

Insert

mVenus-CAGEn-Caax (Nrdj1)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiLox7.0-CAGEc-RFP (Nrdj1)

Plasmid

#241402

Purpose

Proximity-triggered Protein Trans-Splicing between mVenus and RFP, Figure 1 in Nature Chemical Biology 20.10 (2024): 1353-1360.

Depositor

Thomas Muir

Article

Lee et al Nat Chem Biol. 2024 Oct;20(10):1353-1360

Insert

CAGEc-RFP (Nrdj1)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pEF myc-SHP2(Q510L)

Plasmid

#245236

Purpose

Mammalian expression of myc-tagged full-length SHP2

Depositor

Neel Shah

Article

van Vlimmeren et al bioRxiv [Preprint]. 2025 Jul 2:2025.06.3

Insert

SHP2 full-length

Tags

myc

Expression

Mammalian

Mutation

Q510L

Available Since

Oct. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pEF myc-SHP2(Q510R)

Plasmid

#245237

Purpose

Mammalian expression of myc-tagged full-length SHP2

Depositor

Neel Shah

Article

van Vlimmeren et al bioRxiv [Preprint]. 2025 Jul 2:2025.06.3

Insert

SHP2 full-length

Tags

myc

Expression

Mammalian

Mutation

Q510R

Available Since

Oct. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pEF myc-SHP2(R138Q)

Plasmid

#245228

Purpose

Mammalian expression of myc-tagged full-length SHP2

Depositor

Neel Shah

Article

van Vlimmeren et al bioRxiv [Preprint]. 2025 Mar 21:2025.02.

Insert

SHP2 full-length

Tags

myc

Expression

Mammalian

Mutation

R138Q

Available Since

Oct. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pEF myc-SHP2(Q510K)

Plasmid

#245235

Purpose

Mammalian expression of myc-tagged full-length SHP2

Depositor

Neel Shah

Article

van Vlimmeren et al bioRxiv [Preprint]. 2025 Mar 21:2025.02.

Insert

SHP2 full-length

Tags

myc

Expression

Mammalian

Mutation

Q510K

Available Since

Oct. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pEF myc-SHP2(F29K)

Plasmid

#245222

Purpose

Mammalian expression of myc-tagged full-length SHP2

Depositor

Neel Shah

Article

Jiang et al Nat Commun. 2025 Jul 1;16(1):5464. doi:

Insert

SHP2 full-length

Tags

myc

Expression

Mammalian

Mutation

F29K

Available Since

Oct. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pEF myc-SHP2(T52S)

Plasmid

#245226

Purpose

Mammalian expression of myc-tagged full-length SHP2

Depositor

Neel Shah

Article

van Vlimmeren et al bioRxiv [Preprint]. 2025 Jul 2:2025.06.3

Insert

SHP2 full-length

Tags

myc

Expression

Mammalian

Mutation

T52S

Available Since

Oct. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pEF myc-SHP2(Y279C)

Plasmid

#245230

Purpose

Mammalian expression of myc-tagged full-length SHP2

Depositor

Neel Shah

Article

van Vlimmeren et al bioRxiv [Preprint]. 2025 Jul 2:2025.06.3

Insert

SHP2 full-length

Tags

myc

Expression

Mammalian

Mutation

Y279C

Available Since

Oct. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pEF myc-SHP2(E139D)

Plasmid

#245229

Purpose

Mammalian expression of myc-tagged full-length SHP2

Depositor

Neel Shah

Article

van Vlimmeren et al bioRxiv [Preprint]. 2025 Jul 2:2025.06.3

Insert

SHP2 full-length

Tags

myc

Expression

Mammalian

Mutation

E139D

Available Since

Oct. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pEF myc-SHP2(Q510H)

Plasmid

#245234

Purpose

Mammalian expression of myc-tagged full-length SHP2

Depositor

Neel Shah

Article

van Vlimmeren et al bioRxiv [Preprint]. 2025 Jul 2:2025.06.3

Insert

SHP2 full-length

Tags

myc

Expression

Mammalian

Mutation

Q510H

Available Since

Oct. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pEF myc-SHP2(T468M)

Plasmid

#245231

Purpose

Mammalian expression of myc-tagged full-length SHP2

Depositor

Neel Shah

Article

van Vlimmeren et al bioRxiv [Preprint]. 2025 Mar 21:2025.02.

Insert

SHP2 full-length

Tags

myc

Expression

Mammalian

Mutation

T468M

Available Since

Oct. 3, 2025

Availability

Academic Institutions and Nonprofits only

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