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pcDNA5FRTTO-NHASt-codoptUL23

Plasmid

#135104

Purpose

Codon-optimized HSV-1 UL23 with N-terminal HAStrep tag

Depositor

Markus Landthaler

Article

Wyler et al Nat Commun. 2019 Oct 25;10(1):4878. doi:

Insert

HSV-1 UL23 codon optimized

Tags

HA-Strep-Strep (N terminal on backbone)

Expression

Mammalian

Available Since

Jan. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRTTO-codoptUL23-CStHA

Plasmid

#135099

Purpose

Codon-optimized HSV-1 UL23 with C-terminal StrepHA tag

Depositor

Markus Landthaler

Article

Wyler et al Nat Commun. 2019 Oct 25;10(1):4878. doi:

Insert

HSV-1 UL23 codon optimized

Tags

Strep-Strep-HA

Expression

Mammalian

Available Since

Jan. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pEnt R3L2 TetO(sh) mCh-Rs1-2

Plasmid

#24417

Purpose

Human 5HT4B receptor with D100A mutation, generating the RASSL Rs1. Construct expresses both mCherry and Rs1 via a P2A ribosomal skip sequence. Plasmid also known as pEntR3L2 TetO-mCh-Rs1.

Depositor

Edward Hsiao

Article

Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.

Insert

Rs1

Use

Entry vector

Tags

FLAG and mCherry-P2A

Mutation

D100A

Promoter

short TetO, miniCMV

Available Since

March 14, 2011

Availability

Academic Institutions and Nonprofits only

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pCIBN-EYFP-hNCL

Plasmid

#103806

Purpose

BLInCR 'Localizer' construct that marks the nucleoli and is targeted by a PHR-tagged effector upon illumination with blue light

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

CIBN-EYFP-hNCL (CIB1, NCL Mustard Weed, Human)

Expression

Mammalian

Mutation

hNCL: A395G (K132R), A421G (K141E), A424G (K142E)…

Promoter

CMV

Available Since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pMTB2-RanGAP-Cerulean-Avi

Plasmid

#80068

Purpose

Beta-actin2 promoter driving C-term Avi-tagged protein containing Cerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1); flanked by Tol2 sequences

Depositor

Tatjana Sauka-Spengler

Article

Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

Insert

Cerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1) (RANGAP1 Chicken)

Use

Zebrafish biotagging

Tags

Avi

Expression

Bacterial

Promoter

Beta-actin2

Available Since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 myc-mβTrCP WD2-7 (292-569)

Plasmid

#62978

Purpose

Expresses myc-tagged mouse βTrCP residues 292-569

Depositor

Michael Ruppert

Article

Deng et al J Cell Sci. 2015 Jan 27. pii: jcs.162164

Insert

BTRC (Btrc Mouse)

Tags

myc

Expression

Mammalian

Mutation

codes for residues 292-569

Available Since

May 28, 2015

Availability

Academic Institutions and Nonprofits only
pGEX4T-1 hSlp4-a del.SHD

Plasmid

#40067

Depositor

Fernando Martin-Belmonte

Article

Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

Insert

hSlp4-a V21A (SYTL4 Human)

Tags

GST

Expression

Bacterial

Mutation

deleted SHD domain (1-143)

Promoter

Tac

Available Since

Oct. 16, 2012

Availability

Academic Institutions and Nonprofits only
pMIR-Report-Luc-KLF4-K1S

Plasmid

#62979

Purpose

Translational reporter for KLF4

Depositor

Michael Ruppert

Article

Lin et al Mol Cell Biol. 2011 Jun;31(12):2513-27.

Insert

KLF4 partial sequence (KLF4 Human)

Use

Luciferase

Tags

firefly luciferase

Mutation

Fragment 1-446 of KLF4

Promoter

CMV

Available Since

May 28, 2015

Availability

Academic Institutions and Nonprofits only
pTet-O-ATOH7-T2A-PuroR

Plasmid

#162343

Purpose

Lentiviral expression of ATOH7 under the control of the TetON promoter

Depositor

Charles Gersbach

Article

Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

Insert

ATOH7 (ATOH7 Human)

Use

Lentiviral

Tags

T2A-PuroR

Expression

Mammalian

Promoter

Tet-ON

Available Since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2-sgDnmt1_1

Plasmid

#138347

Purpose

Express guide RNA 1 for Dnmt1

Depositor

Yi Zhang

Article

Fu et al Nat Cell Biol. 2019 Jul;21(7):835-844. d

Insert

mouse Dnmt1 sgRNA (Dnmt1 Synthetic)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2-sgDnmt1_2

Plasmid

#138348

Purpose

Express guide RNA 2 for mouse Dnmt1

Depositor

Yi Zhang

Article

Fu et al Nat Cell Biol. 2019 Jul;21(7):835-844. d

Insert

mouse Dnmt1 sgRNA (Dnmt1 Synthetic)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pEF-HA-TSAd-d239-74

Plasmid

#84560

Purpose

mammalian expression of TSAd-d239-74

Depositor

Anne Spurkland

Article

Berge et al PLoS One. 2010 Mar 18;5(3):e9761. doi: 1

Insert

TSAd (SH2D2A Human)

Tags

HA

Expression

Mammalian

Mutation

deletion of aa 239-274

Promoter

EF1a

Available Since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only
EPHB4-gRNA1/2

Plasmid

#139974

Purpose

gRNA used for knockin EGFP into EPHB4 allele

Depositor

James Thomson

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jul 25;11

Insert

EPHB4 gRNA

Expression

Mammalian

Available Since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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EFNB2-gRNA3/4

Plasmid

#139973

Purpose

gRNA used for knockin tdTomado into EFNB2 allele

Depositor

James Thomson

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jul 25;11

Insert

EFNB2-gRNA

Expression

Mammalian

Available Since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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pAGM_Puro_shRNA n.2_mTet3

Plasmid

#79625

Purpose

Expression of shRNA for mouse Tet3

Depositor

Silvia Monticelli

Article

Montagner et al Cell Rep. 2016 May 17;15(7):1566-79. doi

Insert

Tet3 (Tet3 Mouse)

Use

Lentiviral

Tags

none

Available Since

July 21, 2016

Availability

Academic Institutions and Nonprofits only
pAGM_Puro_shRNA n.1_mTet3

Plasmid

#79624

Purpose

Expression of shRNA for mouse Tet3

Depositor

Silvia Monticelli

Article

Montagner et al Cell Rep. 2016 May 17;15(7):1566-79. doi

Insert

Tet3 (Tet3 Mouse)

Use

Lentiviral

Tags

none

Available Since

July 21, 2016

Availability

Academic Institutions and Nonprofits only
pSFG-TFII-I-GFP Y248&249F

Plasmid

#22196

Depositor

Ananda Roy

Article

Desgranges et al Mol Cell Biol. 2005 Dec . 25(24):10940-5

Insert

TFII-I (GTF2I Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

Changed Tyrosines 248 and 249 to Phenylalanines. …

Available Since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only
KA2944_pPB-TREtight-Esrrb-FLAG-InO

Plasmid

#124179

Purpose

piggyBac-based Dox-inducible expression of Esrrb-FLAG

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Insert

Esrrb-FLAG-IRES-H2B-mKO2 (Esrrb Mouse)

Expression

Mammalian

Promoter

tetO-CMVmin

Available Since

July 10, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX4T-1 Slp2-a C2AB

Plasmid

#40058

Depositor

Fernando Martin-Belmonte

Article

Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

Insert

Slp2-a C2AB (Sytl2 Mouse)

Tags

GST

Expression

Bacterial

Mutation

C2AB domains only

Promoter

Tac

Available Since

Nov. 9, 2012

Availability

Academic Institutions and Nonprofits only
pGEX4T-1 hSlp4-a V21A

Plasmid

#40064

Depositor

Fernando Martin-Belmonte

Article

Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

Insert

hSlp4-a V21A (SYTL4 Human)

Tags

GST

Expression

Bacterial

Mutation

Valine 21 to Alanine

Promoter

Tac

Available Since

Oct. 16, 2012

Availability

Academic Institutions and Nonprofits only