We narrowed to 66,054 results for: %s

Showing: 8301 - 8320 of 66054 results

Results per page:

Sort results by:

pEM-Cas9HF1

Plasmid

#89961

Purpose

Modified from pCas9-CR4 (Addgene: 62655) to use the high-fidelity version of Cas9, SpCas9-HF1 (N497A/R661A/Q695A/Q926A) from Kleinstiver et al 2016.

Depositor

Michael Lynch

Publication

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Insert

Cas9HF1

Expression

Bacterial

Mutation

N497A/R661A/Q695A/Q926A

Promoter

pTet

Available since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

Enlarge
Wei lab paired-guide RNA (pgRNA) CRISPR–Cas9 library for human long non-coding RNAs (lncRNAs)

Pooled library

#89640

Purpose

Designed for genomic deletion screening of functional lncRNAs (long non-coding RNAs) using lentiviral pgRNAs (paired-guide RNAs).

Depositor

Wensheng Wei

Publication

Zhu et al Nat Biotechnol. 2016 Dec;34(12):1279-128

Expression

Mammalian

Use

Lentiviral

Available since

May 17, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pCAG-VCre

Plasmid

#89575

Purpose

Expresses VCre under pCAG

Depositor

Wilson Wong

Publication

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

VCre recombinase

Use

Cre/Lox and Synthetic Biology

Tags

NLS

Expression

Mammalian

Available since

April 28, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pSG5-SUV39H2-FL

Plasmid

#89135

Purpose

mammalian expression of full-length 410 amino acid SUV39H2 histone methyltransferase from pSG5, includes 5' and 3' noncoding regions

Depositor

Elizabeth Wilson

Publication

Askew et al Mol Cell Endocrinol. 2017 Mar 5;443:42-5

Insert

SUV39H2 (SUV39H2 Human)

Expression

Mammalian

Promoter

SV40

Available since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pBK-YE2-BE3

Plasmid

#85176

Purpose

Expresses YE2-BE3 in mammalian cells

Depositor

David Liu

Publication

Kim et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Insert

YEE-BE3

Expression

Mammalian

Promoter

CMV

Available since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
p5E-krt5

Plasmid

#82585

Purpose

5' entry vector containing the zebrafish krt5 promoter for epidermis-specific expression

Depositor

Kryn Stankunas

Publication

Akerberg et al PLoS One. 2014 Mar 18;9(3):e92217. doi:

Insert

zebrafish krt5 promoter (krt5 Zebrafish)

Promoter

None

Available since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

Enlarge
pTriEx-RhoA-Q63L_mScarlet-i_SGFP2

Plasmid

#85072

Purpose

Green/red FRET biosensor for RhoA in the GTP locked constitutively active state

Depositor

Dorus Gadella

Publication

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

RhoA Biosensor constitutively active (Q63L) (RHOA Human)

Tags

6xHis and mScarlet-i and SGFP2

Expression

Bacterial, Insect, and Mamm…

Mutation

Q63L

Promoter

CMV

Available since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pKLV2-U6gRNA5(gBFP)-PGKGFP2ABFP-W

Plasmid

#67984

Purpose

Cas9 activity reporter with GFP and BFP

Depositor

Kosuke Yusa

Publication

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Inserts

U6gRNA cassette, PGKGFP2ABFP cassette, WPRE
Guide RNA targeting modified BFP

Use

CRISPR and Lentiviral

Mutation

Deleted BbsI site within WPRE, modified BFP

Available since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

Enlarge
pKLV2-U6gRNA5(BbsI)-PGKpuro2AmAG-W

Plasmid

#67976

Purpose

CRISPR gRNA expression vector with an improved scaffold and puro/mAG markers

Depositor

Kosuke Yusa

Publication

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

U6gRNA cassette, PGKpuro2AmAG cassette, WPRE

Use

Lentiviral

Mutation

Deleted BbsI site within WPRE

Promoter

human U6 promoter, mouse PGK promoter

Available since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

Enlarge
pCDH-EF1-FHC-POLQ-DY2230AA

Plasmid

#64878

Purpose

lentiviral expression of human POLQ -DY2230AA mutant (a polymerase domain mutant)

Depositor

Richard Wood

Publication

Yousefzadeh et al PLoS Genet. 2014 Oct 2;10(10):e1004654.

Insert

POLQ (POLQ Human)

Use

Lentiviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

D2330A,Y2331A, in the DNA polymerase domain (POL)…

Promoter

EF1alpha

Available since

July 11, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

Enlarge
KRas FAK biosensor

Plasmid

#78301

Purpose

FRET biosensor. Visualization of FAK activity at plasma membrane outside lipid rafts in live cells

Depositor

Yingxiao Wang

Publication

Seong et al Nat Commun. 2011 Jul 26;2:406. doi: 10.1

Insert

KRas FAK biosensor

Expression

Mammalian

Promoter

CMV

Available since

June 10, 2016

Availability

Academic Institutions and Nonprofits only
pLH-sgRNA1-boxB-MS2-PP7

Plasmid

#75397

Purpose

sgRNA1-boxB-MS2-PP7

Depositor

Thoru Pederson

Publication

Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

Insert

sgRNA1-boxB-MS2-PP7

Use

CRISPR and Lentiviral

Tags

no

Expression

Mammalian

Promoter

U6

Available since

May 25, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

Enlarge
pLentiCRISPR-RFP657

Plasmid

#75162

Purpose

Derived from LentiCRISPR v1 but expresses RFP657 instead of puromycin resistance

Depositor

Beat Bornhauser

Publication

McComb et al Sci Transl Med. 2016 May 18;8(339):339ra

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

RFP657

Expression

Mammalian

Available since

May 17, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

Enlarge
pRGD-SmR

Plasmid

#74109

Purpose

Plasmid containing streptomycin resistance gene cassette flanked by multiple restriction sites

Depositor

Hans-Martin Fischer

Publication

Ledermann et al Appl Environ Microbiol. 2016 Feb 26. pii

Insert

aadA

Use

Bacterial vector based on pmb1 replicon

Available since

April 15, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-FLAG-MTOR-V2006I

Plasmid

#69012

Purpose

activating MTOR mutation

Depositor

David Sabatini

Publication

Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

Insert

MTOR (MTOR Human)

Tags

FLAG

Expression

Mammalian

Mutation

V2006I

Promoter

CMV

Available since

Jan. 20, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pRL1383a

Plasmid

#70692

Purpose

RSF1010-based vector with extensive polylinker, and resistance to Strep and Spec. Shuttle vector suitable for transfer to, and replication in, E. coli and Anabaena (Nostoc) sp. strain PCC 7120.

Depositor

Peter Wolk

Publication

Wolk et al Arch Microbiol. 2007 Dec;188(6):551-63.

Type

Empty backbone

Use

Bacterial shuttle vector

Available since

Jan. 6, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

Enlarge
pMXs-Puro-ARHGAP26

Plasmid

#69467

Purpose

Expresses ARHGAP26

Depositor

Axel Hillmer

Publication

Yao et al Cell Rep. 2015 Jul 14;12(2):272-85. doi:

Insert

ARHGAP26 (ARHGAP26 Human)

Use

Retroviral

Tags

HA TAG

Available since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

Enlarge
MBP-MEK1

Plasmid

#68300

Purpose

N-terminal MBP tagged human MEK1 gene for bacterial expression

Depositor

Jesse Rinehart

Publication

Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

Insert

MBP-MEK1 (MAP2K1 Synthetic, Human)

Tags

His and MBP

Expression

Bacterial

Available since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
Str-STIM1-NN_ST-SBP-EGFP

Plasmid

#65262

Purpose

synchronize trafficking of ST from the ER (RUSH system)

Depositor

Franck Perez

Publication

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Str-STIM1-NN and truncated sialyltransferase (ST6GAL1 Human)

Tags

EGFP and Streptavidin Binding Protein (SBP)

Expression

Mammalian

Mutation

contains only amino acids 1 to 43 of ST

Promoter

CMV

Available since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

Enlarge
VVT1

Plasmid

#65779

Purpose

The Joung Lab recommends using BPK2660 (Addgene plasmid 70709) instead of VVT1 as guide RNAs cloned into BPK2660 are more effective than this plasmid. Human expression plasmid for S. aureus Cas9 sgRNA

Depositor

Keith Joung

Publication

Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

Insert

SaCas9 gRNA backbone, without spacer sequence

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

Enlarge