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pHH0103_SH3RF2-3/3

Plasmid

#91281

Purpose

Protein expression and purification of human SH3 domain construct SH3RF2-3/3

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SH3RF2-3/3 (SH3RF2 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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WPXL SOX10 gRNA

Plasmid

#101923

Purpose

SOX10 gRNA used for targeted demethylation is inserted into the WPXL lentiviral backbone.

Depositor

Danwei Huangfu

Article

Verma et al Nat Genet. 2018 Jan;50(1):83-95. doi: 10

Insert

SOX10 promoter targeting gRNA

Use

Lentiviral

Available Since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

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CS2-Myc-mASUN

Plasmid

#47044

Purpose

Expression of tagged-mouse ASUN

Depositor

Laura Lee

Article

Jodoin et al Mol Biol Cell. 2012 Dec;23(24):4713-24.

Insert

Asunder (Asun Mouse)

Tags

Myc

Expression

Mammalian

Available Since

Sept. 10, 2013

Availability

Academic Institutions and Nonprofits only
Plxnc1-AP-His

Plasmid

#72005

Purpose

Expresses the extracellular region of the PlexinC1 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Plxnc1 (Plxnc1 Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only

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pCRII-Topo Diap3 in situ probe

Plasmid

#45602

Depositor

Jeffrey Macklis

Article

Arlotta et al Neuron. 2005 Jan 20;45(2):207-21.

Insert

Diap3 in situ probe (Diaph3 Mouse)

Use

In situ

Mutation

fragment contains bp# 1589-2321 of BC028920

Available Since

July 5, 2013

Availability

Academic Institutions and Nonprofits only
pCRII-Topo Crim1 in situ probe

Plasmid

#45600

Depositor

Jeffrey Macklis

Article

Arlotta et al Neuron. 2005 Jan 20;45(2):207-21.

Insert

Crim1 in situ probe (Crim1 Mouse)

Use

In situ

Mutation

insert contains bp#4062-4502 of NM_015800.3

Available Since

July 5, 2013

Availability

Academic Institutions and Nonprofits only
pM3_PLux*R1CI*_PLTetO1R1CI*_PLCR1E2Cr_PJ23102R1LuxR

Plasmid

#250890

Purpose

LuxR expressed under BBa_J23102; E2-Crimson under PLC promoter; mutated cI* under PLTetO1; and cI* under Plux*; part of BNeuAS5; pUC origin of replication

Depositor

Sangram Bagh

Article

Bonnerjee et al Nat Chem Biol. 2024 Nov;20(11):1524-1534

Inserts

E2-Crimson
LuxR
Mutated cI
Mutated cI

Use

Synthetic Biology

Mutation

Frame shifted cI protein and Frame shifted cI pro…

Promoter

BBa_J23102, PLC, PLTetO1, and Plux

Available Since

March 23, 2026

Availability

Academic Institutions and Nonprofits only

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pK1_PLLacO1R3CIw_PLux*R1cI*

Plasmid

#250891

Purpose

Frame shifted cI protein expressed under PLux* promoter; wild type cI expressed under PLLacO1 promoter; component of BNeuAS6; p15A origin of replication

Depositor

Sangram Bagh

Article

Bonnerjee et al Nat Chem Biol. 2024 Nov;20(11):1524-1534

Inserts

Mutated cI
Wild type cI

Use

Synthetic Biology

Mutation

cI* = Frame shifted cI protein; PLux* = Point mut…

Promoter

PLLacO1 and PLux*

Available Since

Feb. 19, 2026

Availability

Academic Institutions and Nonprofits only

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pJC2288 - pET28-mTagBFP-SRT-His6

Plasmid

#250250

Purpose

Recombinant production of sortase-tagged mTagBFP.

Depositor

Jacob Corn

Article

Muhar et al Nature. 2025 Feb;638(8050):519-527. doi:

Insert

mTagBFP

Tags

Sortase recognition site, 6xHis

Expression

Bacterial

Promoter

T7

Available Since

Jan. 28, 2026

Availability

Academic Institutions and Nonprofits only

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LLP1003

Plasmid

#239925

Purpose

sgRNA compatible with TCTP-SynPro-07 and CaMV 35S-SynPro-08 as Level-0 part (Golden Gate cloning)

Depositor

Ryan Lister

Article

Khan et al Nat Biotechnol. 2025 Mar;43(3):416-430.

Insert

sgRNA

Use

Synthetic Biology

Mutation

N/A

Available Since

Jan. 28, 2026

Availability

Academic Institutions and Nonprofits only

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pJC2281 - pHR-SFFV-rtTA3-PGK-Bsr

Plasmid

#250247

Purpose

Expression of reverse tet transactivator for use in the Tet-On system.

Depositor

Jacob Corn

Article

Muhar et al Nature. 2025 Feb;638(8050):519-527. doi:

Insert

rtTA3

Use

Lentiviral

Promoter

SFFV

Available Since

Jan. 15, 2026

Availability

Academic Institutions and Nonprofits only

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pME234

Plasmid

#238104

Purpose

Expresses ME-ABE ABE7.10-HK with SpCas9 (NG

Depositor

Alexis Komor

Article

Evanoff et al Nat Biotechnol. 2026 Mar 18. doi: 10.103

Insert

TadA*7.10(Y123H/N157K)-m_SpnCas9(D10A)

Use

CRISPR

Tags

SV40 bpNLS and SV40 bpNLS-P2A-mCherry

Expression

Mammalian

Mutation

TadA modifications include Y123H, N157K; NG-nCas9…

Available Since

Jan. 9, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV2-Sst44-mTagBFP2

Plasmid

#211807

Purpose

Expresses BFP in a subset of cortical somatostatin neurons.

Depositor

Christopher Harvey

Article

Green et al Nature. 2023 Aug;620(7973):366-373. doi:

Insert

mTagBFP2

Use

AAV

Tags

NLS, FLAG

Expression

Mammalian

Promoter

Sst44 enhancer, modified CMV promoter

Available Since

Jan. 5, 2026

Availability

Academic Institutions and Nonprofits only

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pEP0003_pIVTRup(BsmBI)_ABEmax

Plasmid

#242537

Purpose

IVT

Depositor

Christoph Bock, Paul Datlinger

Article

Datlinger et al Nature. 2025 Oct;646(8086):963-972. doi:

Insert

ABEmax (NGG-ABE)

Use

CRISPR

Mutation

D10A

Available Since

Nov. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pCART0003_pIVTRup(BsmBI)_ABEmax7.10-SpRY

Plasmid

#242539

Purpose

IVT

Depositor

Christoph Bock, Paul Datlinger

Article

Datlinger et al Nature. 2025 Oct;646(8086):963-972. doi:

Insert

ABEmax7.10-SpRY (SpRY-ABE)

Use

CRISPR

Mutation

D10A

Available Since

Nov. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pEP0004_pIVTRup(BsmBI)_AncBE4max

Plasmid

#242538

Purpose

IVT

Depositor

Christoph Bock, Paul Datlinger

Article

Datlinger et al Nature. 2025 Oct;646(8086):963-972. doi:

Insert

AncBE4max (NGG-CBE)

Use

CRISPR

Mutation

D10A

Available Since

Nov. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pEN_TT PCSK5(sgRR,M452I)-V5

Plasmid

#232450

Purpose

Gateway entry vector for an inducible sg09 resistant PCSK5_M452I mutant

Depositor

Kevin Janes

Article

Marohl et al Mol Cancer Res. 2025 Oct 28. doi: 10.115

Insert

PCSK5(M452I) (PCSK5 Human)

Use

Gateway entry vector

Tags

V5

Mutation

a75g_t84c_g87c; M452I

Available Since

Nov. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pEN_TT PCSK5(sgRR,T288P)-V5

Plasmid

#232451

Purpose

Gateway entry vector for an inducible sg09 resistant PCSK5_T288P mutant

Depositor

Kevin Janes

Article

Marohl et al Mol Cancer Res. 2025 Oct 28. doi: 10.115

Insert

PCSK5(T288P) (PCSK5 Human)

Use

Gateway entry vector

Tags

V5

Mutation

a75g_t84c_g87c; T288P

Available Since

Nov. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pSLIK PCSK5(sgRR)-V5 hygro

Plasmid

#232452

Purpose

Lentiviral expression vector for an inducible sg09 resistant PCSK5

Depositor

Kevin Janes

Article

Marohl et al Mol Cancer Res. 2025 Oct 28. doi: 10.115

Insert

PCSK5 (PCSK5 Human)

Use

Lentiviral

Tags

V5

Mutation

a75g_t84c_g87c

Available Since

Nov. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pSLIK PCSK5(sgRR,M452I)-V5 hygro

Plasmid

#232453

Purpose

Lentiviral expression vector for an inducible sg09 resistant PCSK5_M452I mutant

Depositor

Kevin Janes

Article

Marohl et al Mol Cancer Res. 2025 Oct 28. doi: 10.115

Insert

PCSK5(M452I) (PCSK5 Human)

Use

Lentiviral

Tags

V5

Mutation

a75g_t84c_g87c; M452I

Available Since

Nov. 6, 2025

Availability

Academic Institutions and Nonprofits only

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