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pYES2.1-pomSir1

Plasmid

#107447

Purpose

Overexpression of MET fusion protein in S. cerevisiae

Depositor

Clare O'Connor

Article

Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

Insert

Sir1 (sir1 Fission Yeast)

Tags

V5-His

Expression

Yeast

Mutation

H523R, A750T

Promoter

GAL1

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pICSL50015

Plasmid

#50318

Purpose

Please note- this plasmid is currently not distributed in the MoClo Plant Parts Kit (# 1000000047)

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

CDS, mNEON (Branchistoma lanceolatum)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pFGL1258

Plasmid

#116898

Purpose

TetOFF:GFP:CenpA (centromere marker)

Depositor

Naweed Naqvi

Article

Yadav et al MBio. 2019 Jul 30;10(4). pii: mBio.01581

Inserts

CenpA promoter region
CenpA

Use

Fungal expression (in magnaporthe oryzae)

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pHES841

Plasmid

#89194

Purpose

Expresses YFP under control of synthetic promoter containing Zif268 binding sites.

Depositor

Hana El-Samad

Article

Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

Insert

pZ-YFP

Expression

Yeast

Promoter

pZ

Available Since

Jan. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pYES2.1-cerMET16

Plasmid

#107442

Purpose

Overexpression of MET fusion protein in S. cerevisiae

Depositor

Clare O'Connor

Article

Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

Insert

MET16 (MET16 Budding Yeast)

Tags

V5-His

Expression

Yeast

Promoter

GAL1

Available Since

April 21, 2018

Availability

Academic Institutions and Nonprofits only

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pHES874

Plasmid

#89196

Purpose

Expresses mCherry under control of synthetic promoter containing Zif268 binding sites.

Depositor

Hana El-Samad

Article

Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

Insert

pZ-mCherry

Expression

Yeast

Promoter

pZ

Available Since

Oct. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pMLC-BB1

Plasmid

#166922

Purpose

Backbone for assembly of multi-level gene expression controllers

Depositor

Thomas Gorochowski

Article

Greco et al Nat Commun. 2021 Mar 19;12(1):1738. doi:

Insert

CyOFP - Orange fluorescent protein - flanked by BsaI sites

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23100 constitutive

Available Since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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pEPOR0SP0013

Plasmid

#117521

Purpose

Level0 Golden Gate part: CDS, SpCas9-p

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

SpCas9-p (with stop codon)

Use

CRISPR

Available Since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pTHS

Plasmid

#166938

Purpose

Toehold switch multi-level gene expression controller (MLC)

Depositor

Thomas Gorochowski

Article

Greco et al Nat Commun. 2021 Mar 19;12(1):1738. doi:

Insert

Genetic circuit expressing GFP- Toehold switch

Use

Synthetic Biology

Expression

Bacterial

Promoter

PTAC

Available Since

July 13, 2021

Availability

Academic Institutions and Nonprofits only

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pEntry Tshld-GFP

Plasmid

#53211

Purpose

pEntry Tshld-GFP is a product of BP recombination reaction between attB flanked Tshld system-encoded PCR product and pDonor 201 plasmid.

Depositor

Matthew Pratt

Article

Lin et al Chembiochem. 2014 Mar 11. doi: 10.1002/c

Insert

Tshld-GFP

Tags

EGFP, Flag, and HA

Promoter

none

Available Since

May 29, 2014

Availability

Academic Institutions and Nonprofits only

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