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pPAct2noUTR (GB0192)

Plasmid

#68177

Purpose

Provides the Arabidopsis thaliana actin2 gene promoter (without 5' UTR) as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

AtAct2 promoter (no UTR)

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

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CMV-CC2

Plasmid

#51221

Purpose

expression of amino acid 917-1040 of chicken DCTN1 p150Glued in mammalian cells

Depositor

Trina Schroer

Article

Quintyne et al J Cell Biol. 1999 Oct 18;147(2):321-34.

Insert

DCTN1 (DCTN1 Chicken)

Expression

Mammalian

Mutation

aa917-1040

Promoter

CMV

Available Since

March 20, 2014

Availability

Academic Institutions and Nonprofits only
pAc5.1B-EGFP-DmCaf40

Plasmid

#79251

Purpose

Expresses EGFP-tagged DmCaf40 in S2 cells

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

DmCaf40 (Rcd-1 Fly)

Use

Schneider 2 cell expression

Tags

EGFP

Promoter

Ac5 promoter

Available Since

July 19, 2016

Availability

Academic Institutions and Nonprofits only

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pHH0103_NCK2-3/3

Plasmid

#91256

Purpose

Protein expression and purification of human SH3 domain construct NCK2-3/3

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

NCK2-3/3 (NCK2 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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Vh-HA-FRB

Plasmid

#20225

Depositor

Carolyn Bertozzi

Article

Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

Insert

FRB (MTOR Human)

Tags

HA and Vh

Expression

Mammalian

Mutation

T2098L mutation

Available Since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only
YFP(159-238) in pcDNAI/Amp

Plasmid

#60889

Purpose

This vector attaches YFP(159-238) to the N-terminus of a protein.

Depositor

Catherine Berlot

Article

Hynes et al J Biol Chem. 2004 Jul 16;279(29):30279-8

Insert

YFP(159-238)

Expression

Mammalian

Promoter

CMV

Available Since

July 30, 2015

Availability

Academic Institutions and Nonprofits only

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pJBL3664

Plasmid

#162240

Purpose

Transcribes E. coli SRP RNA sequence for SHAPE-Seq

Depositor

Julius Lucks

Article

Yu et al Mol Cell. 2021 Feb 18;81(4):870-883.e10.

Inserts

E. coli SRP RNA
HDV

Expression

Bacterial

Promoter

J23119

Available Since

Dec. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pAAVsc.U6-sgGTTG.CMV/CB-Gluc

Plasmid

#121512

Purpose

Expresses sgRNA targeting the intron in the MADM alleles (GT and TG).

Depositor

Guangping Gao

Article

Wang et al Nat Biotechnol. 2018 Oct;36(9):839-842.

Insert

sgGTTG

Use

AAV

Expression

Mammalian

Promoter

U6

Available Since

July 22, 2019

Availability

Academic Institutions and Nonprofits only

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pET15b-GB

Plasmid

#129689

Purpose

express E. coli GreB

Depositor

Jeff Gelles

Article

Tetone et al Proc Natl Acad Sci U S A. 2017 Feb 14;11

Insert

his6/thrombin/GreB (greB E. coli)

Tags

His6

Expression

Bacterial

Available Since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO DNAJA4

Plasmid

#19467

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

DNAJA4 (DNAJA4 Human)

Expression

Mammalian

Available Since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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pILGFP84

Plasmid

#83542

Purpose

Used to evaluate the expression output of SSA1 promoter with yEGFP used as the reporter gene.

Depositor

Claudia Vickers

Article

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Insert

SSA1 promoter-yEGFP

Expression

Yeast

Promoter

SSA1

Available Since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pscALPS-SIV-COL-BWC01 vpr

Plasmid

#115840

Purpose

Encodes codon optimized 3xFLAG-SIVCOLVpr (KF214240) and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVCOLVpr (KF214240)

Use

Lentiviral

Tags

3x-FLAG

Promoter

SFFV

Available Since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pscALPS-SIV-RCM-vpr

Plasmid

#115838

Purpose

Encodes codon optimized 3xFLAG-SIVRCM Vpr (HM803689) and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVRCM Vpr (HM803689)

Use

Lentiviral

Tags

3x-FLAG

Promoter

SFFV

Available Since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pscALPS-SIV-SUN-K08 vpr

Plasmid

#115832

Purpose

Encodes codon optimized 3xFLAG-SIVSUN Vpr (FR751162) and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVSUN Vpr (FR751162)

Use

Lentiviral

Tags

3x-FLAG

Promoter

SFFV

Available Since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pscALPS-SIV-GSN-CN166 vpr

Plasmid

#115831

Purpose

Encodes codon optimized 3xFLAG SIVGSN-CN166 Vpr (AF468659) and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVGSN-CN166 Vpr (AF468659)

Use

Lentiviral

Tags

3x-FLAG

Promoter

SFFV

Available Since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pscALPS-SIV-SYK vpr

Plasmid

#115821

Purpose

Encodes codon optimized 3xFLAG-SIVSYK Vpr (L06042) and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVSYK Vpr (L06042)

Use

Lentiviral

Tags

3x-FLAG

Promoter

SFFV

Available Since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pscALPS-SIV-TAL vpr

Plasmid

#115820

Purpose

Encodes codon optimized 3xFLAG-SIVTAL Vpr (AM182197) and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVTAL Vpr (AM182197)

Use

Lentiviral

Tags

3x-FLAG

Promoter

SFFV

Available Since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pEPOR1CB0113

Plasmid

#117552

Purpose

Level 1 Golden Gate Cassette: eSaCas9 expression cassette for dicotyledonous plants

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

2x35S+5'UTR OMEGA (pICH51288) + eSaCas9 (no stop codon) (pEPOR0SP0012) + YFP (pICSL50005) + Nos (pICH41421)

Expression

Plant

Promoter

2x35S+5'UTR OMEGA (pICH51288)

Available Since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pHcPro (GB0067)

Plasmid

#68196

Purpose

Provides the CDS of the Tobacco echt virus HcPro protein as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

TEV HcPro coding region

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 26, 2015

Availability

Academic Institutions and Nonprofits only

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p2Flag CMV2-ZO-2-1st-PDZ-mut

Plasmid

#27416

Depositor

Marius Sudol

Article

Oka et al Biochem J. 2010 Nov 25. 432(3):461-72.

Insert

Zonula Occludens 2 (TJP2 Human)

Tags

2xFlag

Expression

Mammalian

Mutation

K38E, F44H, I46E and V48E

Available Since

April 12, 2011

Availability

Academic Institutions and Nonprofits only