We narrowed to 32,277 results for: uros

1. pAAV-nEF-Con/Fon-ChR2-mCherry

   Plasmid

   #137142

   Purpose

   Intersectional viral expression of ChR2-mCherry in cells expressing both Cre AND Flp

   Depositor

   Karl Deisseroth, INTRSECT 2.0 Project

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Has Service

   AAV8

   Insert

   Con/Fon-ChR2-mCherry
   

   Use

   AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

   Mutation

   H134R

   Promoter

   nEF

   Available Since

   June 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-nEF-Con/Foff 2.0-ChR2-EYFP

   Plasmid

   #137163

   Purpose

   Intersectional viral expression of ChR2-EYFP in cells expressing Cre AND NOT Flp

   Depositor

   Karl Deisseroth, INTRSECT 2.0 Project

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Has Service

   AAV8

   Insert

   Con/Foff 2.0-ChR2-EYFP
   

   Use

   AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

   Mutation

   H134R

   Promoter

   nEF

   Available Since

   June 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-nEF-Coff/Fon-ChR2-mCherry

   Plasmid

   #137144

   Purpose

   Intersectional viral expression of ChR2-mCherry in cells expressing Flp AND NOT Cre

   Depositor

   Karl Deisseroth, INTRSECT 2.0 Project

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Has Service

   AAV8

   Insert

   Coff/Fon-ChR2-mCherry
   

   Use

   AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

   Mutation

   H134R

   Promoter

   nEF

   Available Since

   June 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-nEF-Con/Foff 2.0-NpHR3.3-EYFP

   Plasmid

   #137153

   Purpose

   Intersectional viral expression of NpHR3.3-EYFP in cells expressing Cre AND NOT Flp

   Depositor

   Karl Deisseroth, INTRSECT 2.0 Project

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Has Service

   AAV8

   Insert

   Con/Foff 2.0-NpHR3.3-EYFP
   

   Use

   AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

   Mutation

   W179F

   Promoter

   nEF

   Available Since

   June 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-nEF-Con/Fon-ChR2(ET/TC)-EYFP

   Plasmid

   #137139

   Purpose

   Intersectional viral expression of ChR2(ET/TC)-EYFP in cells expressing both Cre AND Flp

   Depositor

   Karl Deisseroth, INTRSECT 2.0 Project

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Has Service

   AAV8

   Insert

   Con/Fon-ChR2(ET/TC)-EYFP
   

   Use

   AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

   Mutation

   E123T, T159C

   Promoter

   nEF

   Available Since

   Aug. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-nEF-Con/Foff 2.0-Arch3.3-EYFP

   Plasmid

   #137149

   Purpose

   Intersectional viral expression of Arch3.3-EYFP in cells expressing Cre AND NOT Flp

   Depositor

   Karl Deisseroth, INTRSECT 2.0 Project

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Has Service

   AAV8

   Insert

   Con/Foff 2.0-Arch3.3-EYFP
   

   Use

   AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

   Mutation

   n/a

   Promoter

   nEF

   Available Since

   June 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-nEF-Con/Foff 2.0-ChR2(ET/TC)-EYFP

   Plasmid

   #137140

   Purpose

   Intersectional viral expression of ChR2(ET/TC)-EYFP in cells expressing Cre AND NOT Flp

   Depositor

   Karl Deisseroth, INTRSECT 2.0 Project

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Has Service

   AAV8

   Insert

   Con/Foff 2.0-ChR2(ET/TC)-EYFP
   

   Use

   AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

   Mutation

   E123T, T159C

   Promoter

   nEF

   Available Since

   June 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMRX-IP-muGFP-hATG2A ΔWIR

   Plasmid

   #215507

   Purpose

   Expresses GFP tagged human ATG2A with the mutation in WIPI interacting region.

   Depositor

   Noboru Mizushima

   Article

   Shimizu et al Hum Mol Genet. 2023 Aug 7;32(16):2623-26

   Insert

   Autophagy related 2A (ATG2A Human)
   

   Use

   Retroviral

   Tags

   muGFP

   Expression

   Mammalian

   Mutation

   P656R (natural variant with no functional relevan…

   Available Since

   June 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. His-PHD2-181-426

   Plasmid

   #223551

   Purpose

   His-tagged human PHD2 catalytic domain with TEV cleavage site between His and PHD2 for PHD2 purification

   Depositor

   Somshuvra Mukhopadhyay

   Article

   Gurol et al Proc Natl Acad Sci U S A. 2024 Jun 25;12

   Insert

   PHD2 (EGLN1 Human)
   

   Tags

   6x His

   Expression

   Bacterial

   Mutation

   Deleted amino acids 1-180.

   Promoter

   trc

   Available Since

   Sept. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. Pvalb-2A-Flpo Flp-in replacement vector

    Plasmid

    #61572

    Purpose

    Recombinase-mediated cassette exchange in ES cells to insert the Flpo recombinase gene at the mouse Pvalb gene stop codon

    Depositor

    Hongkui Zeng

    Article

    Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

    Insert

    Pvalb-2A-Flpo
    

    Use

    Recombinase-mediated cassette exchange using flp …

    Available Since

    Feb. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. PINK1 gRNA (BRDN0001487057)

    Plasmid

    #78037

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PINK1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PINK1 (PINK1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-nEF-Con/Foff 2.0-bREACHes-EYFP

    Plasmid

    #137146

    Purpose

    Intersectional viral expression of bREACHes-EYFP in cells expressing Cre AND NOT Flp

    Depositor

    Karl Deisseroth, INTRSECT 2.0 Project

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Insert

    Con/Foff 2.0-bREACHes-EYFP
    

    Use

    AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

    Mutation

    n/a

    Promoter

    nEF

    Available Since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDONR223_TBK1_WT

    Plasmid

    #82285

    Purpose

    Gateway Donor vector containing TBK1, part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, William Hahn, David Root

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    TBK1 (TBK1 Human)
    

    Use

    Gateway entry vector

    Mutation

    N388D; K570Q

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-nEF-Coff/Fon-Arch3.3-p2a-EYFP

    Plasmid

    #137150

    Purpose

    Intersectional viral expression of Arch3.3-EYFP in cells expressing Flp AND NOT Cre

    Depositor

    Karl Deisseroth, INTRSECT 2.0 Project

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Has Service

    AAV8

    Insert

    Coff/Fon-Arch3.3-p2a-EYFP
    

    Use

    AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

    Mutation

    n/a

    Promoter

    nEF

    Available Since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAAV-nEF-Con/Foff 2.0-ChR2-mCherry

    Plasmid

    #137143

    Purpose

    Intersectional viral expression of ChR2-mCherry in cells expressing Cre AND NOT Flp

    Depositor

    Karl Deisseroth, INTRSECT 2.0 Project

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Has Service

    AAV8

    Insert

    Con/Foff 2.0-ChR2-mCherry
    

    Use

    AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

    Mutation

    H134R

    Promoter

    nEF

    Available Since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDEST53-LRRK2-3XKD-R1441C

    Plasmid

    #25050

    Depositor

    Mark Cookson

    Article

    Greggio et al Neurobiol Dis. 2006 Aug . 23(2):329-41.

    Insert

    LRRK2 (LRRK2 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    "Kinase Dead" Lysine 1906 mutated to A…

    Available Since

    June 17, 2010

    Availability

    Academic Institutions and Nonprofits only

17. EIF2AK2 gRNA (BRDN0001148668)

    Plasmid

    #75639

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human EIF2AK2

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    EIF2AK2 (EIF2AK2 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Cav2.1 EIVA (E1707A) pcDNA3

    Plasmid

    #206125

    Purpose

    expression of rat Cav2.1 calcium channel with a E1686R mutation to enhance expression and a mutation in the P loop of Domain IV to to abolish divalent cation binding

    Depositor

    Annette Dolphin

    Article

    Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

    Insert

    cacna1a (Cacna1a Rat)
    

    Expression

    Mammalian

    Mutation

    E1707A

    Promoter

    CMV

    Available Since

    Oct. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. Cav2.1 HA EIA (E320A) pcDNA3

    Plasmid

    #206124

    Purpose

    expression of rat Cav2.1 calcium channel with a E1686R mutation to enhance expression and a mutation in the P loop of Domain I to abolish divalent cation binding

    Depositor

    Annette Dolphin

    Article

    Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

    Insert

    cacna1a (Cacna1a Rat)
    

    Expression

    Mammalian

    Mutation

    E320A

    Promoter

    CMV

    Available Since

    Oct. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Snap25-LSL-2A-GFP targeting vector

    Plasmid

    #61575

    Purpose

    Target a Cre-dependent GFP expression cassette to the stop codon of the mouse Snap25 gene

    Depositor

    Hongkui Zeng

    Article

    Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

    Insert

    Snap25-LSL-2A-GFP
    

    Use

    Cre/Lox and Mouse Targeting

    Available Since

    Feb. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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