We narrowed to 17,263 results for: LEA

1. pCMV-myc3-Mdm2 p60

   Plasmid

   #52059

   Purpose

   expression of truncated Mdm2 in mammalian cells

   Depositor

   Tyler Jacks, Trudy Oliver

   Article

   Oliver et al Mol Cell. 2011 Jul 8;43(1):57-71. doi: 1

   Insert

   Mdm2 p60 (truncated) (MDM2 Human)
   

   Use

   Tags

   myc3

   Expression

   Mammalian

   Mutation

   p60 (truncated at D367, DVDP motif)

   Promoter

   CMV

   Available since

   March 20, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pAAV-TRE-HTR6-SNAPf-TRIP

   Plasmid

   #189617

   Purpose

   Expresses cilia-targeted RhoA inhibitory peptide, tet-inducible

   Depositor

   David Clapham

   Article

   Sheu et al Cell. 2022 Sep 1;185(18):3390-3407.e18.

   Insert

   HTR6-TRIP_alpha
   

   Use

   AAV

   Tags

   alphaTag in the N-term of HTR6, SNAP-tag in the C…

   Expression

   Mutation

   Promoter

   TRE

   Available since

   May 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pNOC_dCas9_sgRNA NC

   Plasmid

   #176259

   Purpose

   pNOC episomal plasmid harboring the dead version of humanized spCas9 gene sequence tagged with Nlux and spacer sequence on sgRNA is replaced by the type IIS restriction site for endonuclease BaeI and

   Depositor

   John van der Oost

   Article

   Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

   Insert

   dead spCas9
   

   Use

   CRISPR, Luciferase, and Synthetic Biology ; Expre…

   Tags

   luciferase

   Expression

   Mutation

   H840A and D10A mutations on spCas9 to inactivate …

   Promoter

   Available since

   Oct. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAAV-hSyn-mCherry.3xFLAG.NOS1AP-S-WPRE

   Plasmid

   #127867

   Purpose

   pAAV plasmid expressing an mCherry.3xFLAG.NOS1AP-S fusion protein under the hSyn promoter

   Depositor

   Florian Freudenberg

   Article

   Candemir et al Eur Neuropsychopharmacol. 2016 Apr;26(4)

   Insert

   Nos1ap (Nos1ap Human, Mouse)
   

   Use

   AAV

   Tags

   3xFLAG and mCherry

   Expression

   Mutation

   constructed by blunt end fusion of the 5’ 51 nucl…

   Promoter

   hSyn

   Available since

   Aug. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGEX 4T-1 GST-Ago2 S387A

   Plasmid

   #60656

   Purpose

   Expresses GST-Ago2 S387A mutant in mammalian cells

   Depositor

   Carl Novina

   Article

   Horman et al Mol Cell. 2013 May 9;50(3):356-67. doi:

   Insert

   Argonaute 2 (AGO2 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   S387A

   Promoter

   tac

   Available since

   Dec. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAAV-EFS-DIO-GRAB_CRF1.0

   Plasmid

   #208662

   Purpose

   Expresses the genetically-encoded fluorescent corticotropin-releasing factor (CRF) sensor GRAB_CRF1.0 in a cre-dependent manner

   Depositor

   Yulong Li

   Article

   Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

   Insert

   GPCR activation based corticotropin-releasing factor (CRF) sensor GRAB_CRF1.0
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   EFS

   Available since

   Dec. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Recruited polymerase expression - pCMV-T7-ePhi29(+exo)-CC(N5) (LM2982)

   Plasmid

   #208972

   Purpose

   Recruited ePhi29(+exo) DNA polymerase with a C-terminal N5 coiled coil (CC) domain, expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   ePhi29(+exo)-BPNLS-CC(N5)
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   ePhi29(+exo)(M8R/V51A/M97T/G197D/E221K/Q497P/K512…

   Promoter

   CMV and T7

   Available since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCMV-PDGFR-SbMVpC-43LK-iLID(139)-NES-NES-SbMVpN-iLID-SbMVseq-tTA

   Plasmid

   #210503

   Purpose

   expresses LAUNCHERsbmv component in mammalian cells

   Depositor

   Dongmin Lee

   Article

   Cui et al Nat Chem Biol. 2024 Mar;20(3):353-364. d

   Insert

   PDGFR-SbMVpC-43LK-iLID(139)-NES-NES-SbMVpN-iLID-SbMVseq-tTA
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3.1(+)-RasAR(R89L)-NES

   Plasmid

   #205568

   Purpose

   RasAR negative-control mutant plasmid. Targeted to cytosol.

   Depositor

   Jin Zhang

   Article

   Weeks et al J Am Chem Soc. 2022 Sep 28;144(38):17432

   Insert

   RasAR(R89L)-NES (RAF1 Human, Synthetic)
   

   Use

   Tags

   Nuclear export signal (NES)

   Expression

   Mammalian

   Mutation

   Arg 89 mutated to Leu in Raf1 RBD region.

   Promoter

   CMV

   Available since

   Oct. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pHAGE2-TetOminiCMV-SK

    Plasmid

    #136577

    Purpose

    Dox-inducible polycistronic lentiviral vector expressing mouse Sox2, Klf4

    Depositor

    Hans Schöler

    Article

    Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

    Insert

    Sox2-T2A-Klf4 (Klf4, Sox2 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    tetO-miniCMV (dox-inducible)

    Available since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pelB-MBP-BB0323-ss

    Plasmid

    #137067

    Purpose

    E. coli expression clone (T7lac promoter) for mature BB0323 (aa 20-377) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavage

    Depositor

    Debra Hansen

    Article

    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

    Insert

    AAC66700.1
    

    Use

    Tags

    PelB signal peptide + His10 + maltose binding pro…

    Expression

    Bacterial

    Mutation

    mature BB0323: lacks the BB0323 signal peptide

    Promoter

    T7lac

    Available since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLKO5-SgHottip-GFP-CRISPRa

    Plasmid

    #134990

    Purpose

    dCas9-mediated activation of HOTTIP in mammalian cells

    Depositor

    Suming Huang

    Article

    Luo et al Cancer Cell. 2019 Dec 9;36(6):645-659.e8

    Insert

    HOTTIP (HOTTIP Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. NFATc1-CRISPR #1 (PX458-EF1a-pSpCas9(BB)-2A-GFP)

    Plasmid

    #75236

    Purpose

    CRISPR/Cas9 plasmid against human NFATc1

    Depositor

    Ria Baumgrass

    Article

    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

    Insert

    sgRNA against NFATc1 (NFATC1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAAV-hSyn-mCherry.3xFLAG.NOS1AP396-500-WPRE

    Plasmid

    #174134

    Purpose

    pAAV plasmid expressing an mCherry.3xFLAG.NOS1AP396-500 fusion protein under the hSyn promoter

    Depositor

    Florian Freudenberg

    Article

    Freudenberg et al EBioMedicine. 2021 Aug 26;71:103565. doi

    Insert

    Nos1ap (Nos1ap Mouse)
    

    Use

    AAV

    Tags

    3xFLAG and mCherry

    Expression

    Mutation

    Only contains the sequences coding for amino acid…

    Promoter

    hSyn

    Available since

    Oct. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMIG-hH4-AVITEV

    Plasmid

    #74051

    Purpose

    retroviral expression plasmid for human histone H4 with C-terminal BirA-biotinylation signal and TEV celavage site

    Depositor

    Ria Baumgrass

    Article

    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

    Insert

    human Histone-H4 (H4C16 Human)
    

    Use

    Retroviral

    Tags

    AVI-TEV

    Expression

    Mammalian

    Mutation

    Promoter

    pMSCV-LTRs

    Available since

    June 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. NFATc1-CRISPR #2 (PX458-EF1a-pSpCas9(BB)-2A-GFP)

    Plasmid

    #75237

    Purpose

    CRISPR/Cas9 plasmid against human NFATc1

    Depositor

    Ria Baumgrass

    Article

    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

    Insert

    sgRNA against NFATc1 (NFATC1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pHR-FUSN-miRFP670-Cry2WT

    Plasmid

    #122441

    Purpose

    Expresses disordered protein FUS(1-214) fused with fluorescent protein miRFP670 and optogenetic protein Cry2WT

    Depositor

    Clifford Brangwynne

    Article

    Shin et al Cell. 2018 Nov 29;175(6):1481-1491.e13.

    Insert

    FUS (FUS Human)
    

    Use

    Lentiviral

    Tags

    Cry2WT and miRFP670

    Expression

    Mammalian

    Mutation

    Contains only amino acids 1-214

    Promoter

    SFFV

    Available since

    March 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3.1(+)-C-eGFP-Ctdnep1_D67EsiR

    Plasmid

    #196518

    Purpose

    Expression of Ctdnep1_D67E-GFP

    Depositor

    Edgar Gomes

    Article

    Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

    Insert

    Ctdnep1_D67EsiR (CTDNEP1 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    D67E mutation (phosphastase dead). Silent mutatio…

    Promoter

    CMV promoter; T7 promoter

    Available since

    March 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. mature ISG15 C-term. domain (79-157)

    Plasmid

    #110764

    Purpose

    Bacterial expression of proISG15 C-term. domain

    Depositor

    David Komander

    Article

    Swatek et al Proc Natl Acad Sci U S A. 2018 Mar 6;115

    Insert

    ISG15 (ISG15 Human)
    

    Use

    Tags

    N-His6-3C

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSUMO-HsSyx3

    Plasmid

    #179288

    Purpose

    E. coli expression plasmid (T7 promoter) for expression-optimized DNA of human Syx (aa 393-792) with N-terminal His6 + SUMO + TEV protease cleavage site

    Depositor

    Debra Hansen

    Article

    Boyd et al FASEB J. 2022 Jul;36(7):e22378. doi: 10.

    Insert

    Syx
    

    Use

    Tags

    His6 + SUMO + TEV protease cleavage site

    Expression

    Bacterial

    Mutation

    residues 393-792 from accession number NP_0010361…

    Promoter

    T7

    Available since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge