We narrowed to 15,951 results for: grna

1. Perturb-FISH

   Plasmid

   #220626

   Purpose

   expression of gRNAs under U6T7 promoter

   Depositor

   Samouil Farhi

   Article

   Binan et al Cell. 2025 Mar 10:S0092-8674(25)00197-7.

   Insert

   sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   BsmBI

   Available since

   March 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. SiC-V2-Cas9G7

   Plasmid

   #133043

   Purpose

   Doxycycline-inducible SiC-V2 vector with Cas9G7 (sgRNA 7 targeting SpCas9 gene). In cells coexpressing Cas9 nuclease, this construct causes self-inactivation/editing of SpCas9 gene upon Dox addition.

   Depositor

   Sriram Neelamegham

   Article

   Kelkar et al Mol Ther. 2019 Sep 12. pii: S1525-0016(1

   Insert

   Tet repressor
   

   Use

   Lentiviral

   Tags

   Cerulean

   Expression

   Mutation

   Promoter

   Available since

   Nov. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pX330-PITCh-H3C2

   Plasmid

   #207780

   Purpose

   Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of H3C2 for knock-in.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   sgRNA Targeting C-terminus of H3C2 (H3C2 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Dec. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. LentiGuide-Neo

   Plasmid

   #139449

   Purpose

   Lentiviral vector with gRNA scaffold and neomycin selectable marker

   Depositor

   Caroline Goujon

   Article

   Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

   Insert

   no sgRNA inserted; resistance gene: neoR
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a / U6

   Available since

   Aug. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. p-dCas9-SSAP-MS2-BB_BbsI

   Plasmid

   #183826

   Purpose

   pU6-MS2-gRNA-backbone(BbsI)-CBH-dSpCas9-T2A-EBFP

   Depositor

   Le Cong

   Article

   Wang et al Nat Cell Biol. 2022 Feb;24(2):268-278. d

   Insert

   U6-guideRNA-dCas9-T2A-EBFP
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CBH

   Available since

   May 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCRISPEY-GAL-Kan-ADE2

   Plasmid

   #232105

   Purpose

   Expresses galactose-inducible CRISPR guide RNA and repair template for creating a frameshift mutation in ADE2 gene of yeast.

   Depositor

   Jeffrey Lewis

   Article

   Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

   Insert

   ADE2 gRNA and repair template
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Repair template introduces C at nt 466 of ADE2 to…

   Promoter

   GAL7

   Available since

   Feb. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pT040

   Plasmid

   #67640

   Purpose

   URA3 vector for cloning guide sequences using BclI-SwaI sites into guide RNA expression cassette

   Depositor

   John Wyrick

   Article

   Laughery et al Yeast. 2015 Dec;32(12):711-20. doi: 10.1

   Insert

   single guide RNA expression cassette
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   pSNR52

   Available since

   Aug. 31, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMOD_B2103

   Plasmid

   #91061

   Purpose

   Module B, Promoter: CmYLCV, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers , Terminator: 35S

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   CmYLCV

   Available since

   July 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pORANGE GFP-Grin1 KI #3

   Plasmid

   #139659

   Purpose

   Endogenous tagging of GluN1: N-terminal (amino acid position: A20)

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   gRNA and GFP donor
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and Cbh

   Available since

   May 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pORANGE GFP-Grin1 KI #2

    Plasmid

    #139658

    Purpose

    Endogenous tagging of GluN1: N-terminal (amino acid position: D23)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and Cbh

    Available since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pX330-PITCh-TUBA1B

    Plasmid

    #207763

    Purpose

    Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the N-terminus of TUBA1B for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting N-terminus of TUBA1B (TUBA1B Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. LentiCRISPRv2puro-Ptgs2_B6

    Plasmid

    #170300

    Purpose

    A knock-out vector for the mouse Ptgs2 in C57BL6

    Depositor

    Michiyuki Matsuda

    Article

    Konishi et al Cancer Res. 2021 May 25. pii: 0008-5472.

    Insert

    A gRNA targeting the mouse tyrosinase gene.
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. LentiCRISPRv2hyg-Ptgs1

    Plasmid

    #170299

    Purpose

    A knock-out vector for the mouse Ptgs1

    Depositor

    Michiyuki Matsuda

    Article

    Konishi et al Cancer Res. 2021 May 25. pii: 0008-5472.

    Insert

    A gRNA targeting the mouse Ptgs1 gene.
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. Cas9-GFP_sg_mTfeb

    Plasmid

    #79006

    Purpose

    Expresses WT Cas9 and GFP, along with a sgRNA to mouse Tfeb.

    Depositor

    Reuben Shaw

    Article

    Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

    Insert

    sgTfeb (Tfeb Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    June 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLKO5.U6.DR130(CasRX)_Empty.EFS.tRFP657

    Plasmid

    #212961

    Purpose

    Lentiviral plasmid for the cloning of mature gRNAs of the RfxCas13d (CasRx) CRISPR system. As a marker, the plasmid also encodes the tRFP657 fluorescence protein.

    Depositor

    Roland Rad

    Article

    Montero et al Nat Methods. 2024 Feb 26. doi: 10.1038/s

    Insert

    DR1_CasRx_backbone
    

    Use

    CRISPR and Lentiviral

    Tags

    TagRFP657

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLC-mCherry-MLKL

    Plasmid

    #75167

    Purpose

    LentiCRISPR-mCherry with sgRNA targeting human MLKL

    Depositor

    Beat Bornhauser

    Article

    McComb et al Sci Transl Med. 2016 May 18;8(339):339ra

    Insert

    MLKL sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMOD_B2303

    Plasmid

    #91068

    Purpose

    Module B, Promoter: CmYLCV, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with tRNA spacers , Terminator: 35S

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    SapI ccdb cassette for cloning multiple gRNA spacers with tRNA spacers
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    CmYLCV

    Available since

    July 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDIRECT_25F

    Plasmid

    #91143

    Purpose

    Direct Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:TaCas9 + TaU6:gRNA, Plant Selection: PvUbi2:hpt II

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Engineering Reagent: ZmUbi:TaCas9 + TaU6:gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. hCas9-VPR

    Plasmid

    #68497

    Purpose

    nuclease competent SP-Cas9 fused to VPR

    Depositor

    George Church

    Article

    Kiani et al Nat Methods. 2015 Sep 7. doi: 10.1038/nm

    Insert

    SP-Cas9-VPR
    

    Use

    Tags

    VPR

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLC-EGFP-RIP3

    Plasmid

    #75165

    Purpose

    LentiCRISPR-EGFP with sgRNA targeting human RIP3

    Depositor

    Beat Bornhauser

    Article

    McComb et al Sci Transl Med. 2016 May 18;8(339):339ra

    Insert

    RIP3 sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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