Addgene

We narrowed to 16,288 results for: IGH@

Showing: 821 - 840 of 16288 results

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Sniper2P

Plasmid

#193857

Purpose

Expresses human codon-optimized Sniper2P and blasticidin resistance: EFS promoter-Sniper2P-NLS-FLAG-P2A-BSD

Depositor

Hyongbum Kim

Article

Kim et al Nat Chem Biol. 2023 Mar 9. doi: 10.1038/

Insert

Sniper2L

Use

CRISPR and Lentiviral

Tags

BSD, FLAG, and NLS

Expression

Mammalian

Mutation

E1007L

Promoter

EFS

Available since

March 24, 2023

Availability

Academic Institutions and Nonprofits only

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ShadowY-CLC

Plasmid

#193560

Purpose

Expresses human Clathrin Light Chain B tagged with ShadowY in mammalian cells.

Depositor

Justin Taraska

Article

Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

Insert

Clathrin light chain B (CLTB Human)

Use

Tags

ShadowY

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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CLC-ShadowY

Plasmid

#193561

Purpose

Expresses human Clathrin Light Chain B tagged with ShadowY in mammalian cells.

Depositor

Justin Taraska

Article

Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

Insert

Clathrin light chain B (CLTB Human)

Use

Tags

ShadowY

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pT3TS-Hypa-nCas9n-A69

Plasmid

#125645

Purpose

micro-injection of poly-adenylated Hypa-nCas9n RNA

Depositor

Jason Berman

Article

Prykhozhij et al Methods Mol Biol. 2020;2115:385-405. doi

Insert

Hypa-nCas9n

Use

Rna in vitro transcription vector

Tags

Expression

Mutation

N692A, M694A, Q695A, H698A from wild-type Cas9

Promoter

T3

Available since

July 11, 2019

Availability

Academic Institutions and Nonprofits only

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pRevLCav2deltaC

Plasmid

#60809

Purpose

to make truncation of yeast Rev3

Depositor

Youri Pavlov, Tahir Tahirov

Article

Siebler et al DNA Repair (Amst). 2014 May 9. pii: S156

Insert

REV3 (REV3 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

rev3ΔC encodes for Rev3 lacking the C-terminus (a…

Promoter

Available since

March 9, 2015

Availability

Academic Institutions and Nonprofits only

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pQLL-Mariner2_AG

Plasmid

#217063

Purpose

Donor plasmid of Mariner2_AG Transposon

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

TIRs of Mariner2_AG transposon

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pRetroX-PTuner DD-M.EcoGII-v5-Telomeric repeat-binding-factor1

Plasmid

#122084

Purpose

Express M.EcoGII fused to TRF1 with detabilization domain - inducible

Depositor

Laure Crabbe

Article

Sobecki et al Cell Rep. 2018 Dec 4;25(10):2891-2903.e5

Insert

DD-linker-M.EcoGII-V5-TERF1

Use

Retroviral

Tags

V5 tag on Nter of TERF1

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

May 2, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-X1-Neo-DDRGK1-WT-HA

Plasmid

#139845

Purpose

Lentiviral expression of human DDRGK1-WT-HA

Depositor

Jacob Corn

Article

Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

Insert

DDRGK1 (DDRGK1 Human)

Use

Lentiviral

Tags

HA

Expression

Mutation

Promoter

Available since

July 6, 2020

Availability

Academic Institutions and Nonprofits only

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TetOn-CLIMP63-mCherry-eGFP

Plasmid

#139871

Purpose

Dox-inducible lentiviral expression of human CLIMP63

Depositor

Jacob Corn

Article

Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

Insert

CLIMP63 (CKAP4 Human)

Use

Lentiviral

Tags

mCherry-eGFP

Expression

Mutation

Promoter

Available since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-Mariner2_AG

Plasmid

#217135

Purpose

Mammalian expression vector for expression of Mariner2_AG transposase (CMV promoter)

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

Mariner2_AG transposase

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pGreenFire_blastocidin

Plasmid

#174103

Purpose

pGreenFire MPRA plasmid for cloning

Depositor

Paul Khavari

Article

Guo et al Nat Genet. 2023 Nov;55(11):1876-1891. do

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Aug. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pBW2426_pCAG-PV1-iCre-N270-L1-GID1-NLS-BGHpA

Plasmid

#108729

Purpose

Expresses N-terminus to aa270 of Cre recombinase fused to GID1 CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

Cre recombinase fragment N-terminus to aa270

Use

Cre/Lox and Synthetic Biology

Tags

GID1,NLS

Expression

Mammalian

Mutation

Promoter

pCAG

Available since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pGY577

Plasmid

#166663

Purpose

Light-inducible Cre recombinase (LiCre) lentiviral expression plasmid for light-induced Cre/Lox recombination

Depositor

Gaël Yvert

Article

Duplus-Bottin et al Elife. 2021 Feb 23;10. pii: 61268. doi:

Insert

LiCre

Use

Cre/Lox and Lentiviral

Tags

Expression

Mammalian

Mutation

Cre: E340A, D341A

Promoter

CMV

Available since

April 26, 2021

Availability

Academic Institutions and Nonprofits only

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pBR_attB(bxb)_ccdB_lox

Plasmid

#183763

Purpose

Bxb1-specific donor plasmid for the cloning of multiple inserts by Golden Gate assembly (BpiI)

Depositor

Richard Davis

Article

Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

Insert

ccdB

Use

Cre/Lox and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 13, 2022

Availability

Academic Institutions and Nonprofits only

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Bxb1_AJMA

Plasmid

#183760

Purpose

Bxb1-specific donor plasmid for integrating transcription units for expressing ASAP2f (2 copies), jRCaMP1b and membrane-localised miRFP703

Depositor

Richard Davis

Article

Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

Inserts

ASAP2f
jRCaMP1b
miRFP703
ASAP2f

Use

Tags

Lck and NES

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pBW2436_pCAG-PV1-GAI-L1-Cre-271C-NLS-BGHpA

Plasmid

#108730

Purpose

Expresses aa271 to C-terminus of Cre recombinase fused to GAI CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

Cre recombinase fragment aa271 to C-terminus

Use

Cre/Lox and Synthetic Biology

Tags

GAI and NLS

Expression

Mammalian

Mutation

Promoter

pCAG

Available since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pENTR-eGFP-attP(bxb)-*BsdR

Plasmid

#183751

Purpose

Bxb1 landing pad cassette.

Depositor

Richard Davis

Article

Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

Insert

loxP-eGFP-attP-Bsd-lox251

Use

Cre/Lox and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 13, 2022

Availability

Academic Institutions and Nonprofits only

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HaloTag-WDR5

Plasmid

#200880

Purpose

Mammalian expression of human WDR5 with N-terminal HaloTag fusion

Depositor

Stefan Knapp

Article

Schwalm et al Cell Chem Biol. 2023 Jun 20:S2451-9456(2

Insert

HaloTag-WDR5 (WDR5 Human)

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

Available since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-X1-Neo-HA-CCPG1

Plasmid

#139867

Purpose

Lentiviral expression of HA-tagged human CCPG1

Depositor

Jacob Corn

Article

Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

Insert

CCPG1 (CCPG1 Human)

Use

Lentiviral

Tags

HA

Expression

Mutation

Promoter

Available since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-ArchT-GFP

Plasmid

#29777

Purpose

AAV-mediated expression of ArchT-GFP for neuronal inhibition

Depositor

Edward Boyden

Article

Han et al Front Syst Neurosci. 2011;5:18. Epub 201

Insert

ArchT-GFP

Use

AAV; Adeno-associated virus

Tags

GFP

Expression

Mammalian

Mutation

N/A

Promoter

CAG

Available since

July 7, 2011

Availability

Academic Institutions and Nonprofits only

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