We narrowed to 13,276 results for: SHI

1. pCS2plus-TDP-43

   Plasmid

   #162608

   Purpose

   Allows for transcription of TDP-43 mRNA for injection into zebrafish embryos for BiFC assays

   Depositor

   Marco Morsch

   Article

   Don et al Mol Neurobiol. 2021 May;58(5):2061-2074.

   Insert

   TARDBP (TARDBP Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFastBac1_GI.1_A37C-A44C

   Plasmid

   #162582

   Purpose

   Expresses norovirus GI.1 VP1 protein with mutations A37C-A44C in Sf9 insect cells

   Depositor

   Peter Kwong

   Article

   Verardi et al NPJ Vaccines. 2020 Dec 14;5(1):110. doi:

   Insert

   VP1 protein from human norovirus GI.1
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Alanine 37 changed to Cysteine and Alanine 44 cha…

   Promoter

   polyhedring

   Available since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFastBac1_GI.1_A37I-A44L

   Plasmid

   #162583

   Purpose

   Expresses norovirus GI.1 VP1 protein with mutations A37I-A44L in Sf9 insect cells

   Depositor

   Peter Kwong

   Article

   Verardi et al NPJ Vaccines. 2020 Dec 14;5(1):110. doi:

   Insert

   VP1 protein from human norovirus GI.1
   

   Use

   Tags

   Expression

   Insect

   Mutation

   A37I and A44L

   Promoter

   polyhedrin

   Available since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFastBac1_GI.1_Q141V-P221L

   Plasmid

   #162584

   Purpose

   Expresses norovirus GI.1 VP1 protein with mutations Q141V-P221L in Sf9 insect cells

   Depositor

   Peter Kwong

   Article

   Verardi et al NPJ Vaccines. 2020 Dec 14;5(1):110. doi:

   Insert

   VP1 protein from human norovirus GI.1
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Glutamine 141 changed to Valine and Proline 221 c…

   Promoter

   polyhedrin

   Available since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFastBac1_GI.1_L144C-P221C

   Plasmid

   #162585

   Purpose

   Expresses norovirus GI.1 VP1 protein with mutations L144C-P221C in Sf9 insect cells

   Depositor

   Peter Kwong

   Article

   Verardi et al NPJ Vaccines. 2020 Dec 14;5(1):110. doi:

   Insert

   VP1 protein from human norovirus GI.1
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Leucine 144 changed to Cysteine and Proline 221 c…

   Promoter

   polyhderin

   Available since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFastBac1_GI.1_G131C-N172C

   Plasmid

   #162586

   Purpose

   Expresses norovirus GI.1 VP1 protein with mutations G131C-N172C in Sf9 insect cells

   Depositor

   Peter Kwong

   Article

   Verardi et al NPJ Vaccines. 2020 Dec 14;5(1):110. doi:

   Insert

   VP1 protein from human norovirus GI.1
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Glycine 131 changed to Cysteine and Asparagine 17…

   Promoter

   polyhedrin

   Available since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFastBac1_GI.1_N167C-L169C

   Plasmid

   #162587

   Purpose

   Expresses norovirus GI.1 VP1 protein with mutations N167C-L169C in Sf9 insect cells

   Depositor

   Peter Kwong

   Article

   Verardi et al NPJ Vaccines. 2020 Dec 14;5(1):110. doi:

   Insert

   VP1 protein from human norovirus GI.1
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Asparagine 167 changed to Cysteine and Leucine 16…

   Promoter

   polyhedrin

   Available since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. LentiV_Neo_ZFP64_Delta_TAD

   Plasmid

   #118698

   Purpose

   Express ZFP64 without transcription activation domain(TAD)

   Depositor

   Christopher Vakoc

   Article

   Lu et al Cancer Cell. 2018 Dec 10;34(6):970-981.e

   Insert

   ZFP64_Delta_TAD (ZFP64 Human)
   

   Use

   Lentiviral

   Tags

   3XFLAG was fused to the N terminal of ZFP64 cDNA

   Expression

   Mutation

   Delete amino acid from 453 to 681

   Promoter

   Available since

   Dec. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti-VRER-P2A-GFP-PGK-Puro

   Plasmid

   #110862

   Purpose

   Lentiviral vector for constitutive expression of Cas9-VRER-P2A-GFP (not codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   Cas9-VRER
   

   Use

   Lentiviral

   Tags

   3X FLAG

   Expression

   Mutation

   D1135V, G1218R, R1335E, T1337R

   Promoter

   EF1s

   Available since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti-HF1-P2A-GFP-PGK-Puro

    Plasmid

    #110863

    Purpose

    Lentiviral vector for constitutive expression of Cas9-HF1-P2A-GFP (not codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    Cas9-HF1
    

    Use

    Lentiviral

    Tags

    3X FLAG

    Expression

    Mutation

    N497A, R661A, Q695A, Q926A

    Promoter

    EF1s

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCOG40-HRS (257-276) Hs

    Plasmid

    #83236

    Purpose

    pGST-HRS (257-276) Hs, Expresses the GST-HRS in E.coli

    Depositor

    Gali Prag

    Article

    Levin-Kravets et al Nat Methods. 2016 Nov;13(11):945-952. do

    Insert

    HRS (257-276) Hs (HGS Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    258-277

    Promoter

    Available since

    Nov. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLBCX-Large-T-antigenK1 mutant

    Plasmid

    #189978

    Purpose

    Retroviral vector used to immortalize human cells

    Depositor

    William Kaelin

    Article

    Shi et al Cancer Cell. 2022 Sep 12;40(9):939-956.e

    Insert

    Large-T-antigenK1 mutant
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMSCV-MerCreMer-hygro

    Plasmid

    #188982

    Purpose

    Retroviral expression of tamoxifen-inducible Cre recombinase

    Depositor

    William Kaelin

    Article

    Shi et al Cancer Cell. 2022 Sep 12;40(9):939-956.e

    Insert

    Tamoxifen-inducible Cre recombinase
    

    Use

    Cre/Lox and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV2-sgTrp53-sgAtrx-EFS-Cre

    Plasmid

    #189977

    Purpose

    Expresses Cre cDNA and sgRNAs targeting murine Trp53 and Atrx

    Depositor

    William Kaelin

    Article

    Shi et al Cancer Cell. 2022 Sep 12;40(9):939-956.e

    Insert

    sgTrp53, sgAtrx, Cre recombinase
    

    Use

    AAV, Cre/Lox, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. Human DNA Binding Domain-Focused CRISPR Knockout Library

    Pooled Library

    #123334

    Purpose

    Expresses gRNA targeting the DNA binding domain of human transcription factors.

    Depositor

    Christopher Vakoc

    Article

    Huang et al Genes Dev. 2018 Jul 1;32(13-14):915-928.

    Expression

    Mammalian

    Species

    Homo sapiens

    Use

    CRISPR and Lentiviral

    Available since

    May 2, 2019

    Availability

    Academic Institutions and Nonprofits only

16. pAAV-hSyn-ASAP3-WPRE

    Plasmid

    #132331

    Purpose

    expresses ASAP3(ASAP-family genetically encoded voltage indicator) in mammalian cells, gene delivered by pAAV-hSyn vector

    Depositor

    Michael Lin

    Article

    Villette et al Cell. 2019 Dec 12;179(7):1590-1608.e23.

    Insert

    ASAP3
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSynapsin

    Available since

    Dec. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. gCBEv2

    Plasmid

    #220617

    Purpose

    vector for encoding an engineered glycosylase-based cytosine (C) base editor (gCBEv2) driven by EF1a promoter, guide RNAs compatible with SpCas9 driven by hU6, mCherry driven by CBh promoter

    Depositor

    Huawei Tong

    Article

    Tong et al Nat Commun. 2024 Jun 8;15(1):4897. doi:

    Insert

    hUNG2-nSpCas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    hUNG2(del1-88;N213D,K184A,A214V), SpCas9(D10A)

    Promoter

    hU6, EF1a

    Available since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. gGBEv6.3-SpCas9

    Plasmid

    #202629

    Purpose

    vector for encoding an engineered glycosylase-based guanine base editor (gGBEv6.3) with SpCas9 nickase driven by EF1a promoter, guide RNA compatible with SpCas9 driven by hU6, mCherry driven by CBh promoter

    Depositor

    Huawei Tong

    Article

    Tong et al Natl Sci Rev. 2023 May 16;10(8):nwad143.

    Insert

    nSpCas9(D10A)-MPGv6.3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    MPGv6.3 = G163R, N169G, D175R, C178N, S198A, K202…

    Promoter

    hU6, EF1a, CBh

    Available since

    June 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCBh_3x Flag-NLS-enOsCas12f1-NLS_pA_phU6_gRNA scaffold-spacer_pCMV_mCherry_pA

    Plasmid

    #197026

    Purpose

    Vector encoding a human codon-optimized enOsCas12f1 driven by CBh promoter, guide RNAs compatible with enOsCas12f1 driven by hU6, and mCherry driven by CMV promoter

    Depositor

    Xiangfeng Kong

    Article

    Kong et al Nat Commun. 2023 Apr 11;14(1):2046. doi:

    Insert

    humanized enOsCas12f1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D52R / T132R

    Promoter

    CBh, CMV, hU6

    Available since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCBh_3x Flag-NLS-enRhCas12f1-NLS_pA_phU6_gRNA scaffold-spacer_pCMV_mCherry_pA

    Plasmid

    #197027

    Purpose

    Vector encoding a human codon-optimized enRhCas12f1 driven by CBh promoter, guide RNAs compatible with enRhCas12f1 driven by hU6, and mCherry driven by CMV promoter

    Depositor

    Xiangfeng Kong

    Article

    Kong et al Nat Commun. 2023 Apr 11;14(1):2046. doi:

    Insert

    humanized enRhCas12f1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    L270R

    Promoter

    CBh, CMV, hU6

    Available since

    May 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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