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pBad-Xis/Int Reset Flipper

Plasmid

#38207

Depositor

Drew Endy

Article

Bonnet et al Proc Natl Acad Sci U S A. 2012 Jun 5;109

Inserts

Bxb1-Excisionase
Bxb1 Integrase

Use

Synthetic Biology; Biobrick cloning vector

Promoter

pBAD

Available Since

July 30, 2012

Availability

Academic Institutions and Nonprofits only

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pBad-Int Set Flipper

Plasmid

#38209

Depositor

Drew Endy

Article

Bonnet et al Proc Natl Acad Sci U S A. 2012 Jun 5;109

Insert

Bxb1 Integrase

Use

Synthetic Biology; Biobrick cloning vector

Tags

His

Promoter

pBAD/AraC

Available Since

July 30, 2012

Availability

Academic Institutions and Nonprofits only

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Ii-Str_neomycin

Plasmid

#65308

Purpose

Luminal ER hook only (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Invariant chain (p33)

Expression

Mammalian

Mutation

Streptavidin in its lumenal part

Promoter

CMV

Available Since

Aug. 26, 2015

Availability

Academic Institutions and Nonprofits only

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pME-Gal4

Plasmid

#53366

Purpose

full-length native Gal4 with stop in Gateway middle entry clone

Depositor

Joshua Bonkowsky

Article

Fujimoto et al Dev Dyn. 2011 Oct;240(10):2324-34. doi:

Insert

native (full-length) Gal4

Promoter

no promoter

Available Since

May 16, 2014

Availability

Academic Institutions and Nonprofits only

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CMV-VARNAM

Plasmid

#115552

Purpose

Red fluorescent voltage indicator for mammalian expression

Depositor

Vincent Pieribone

Article

Kannan et al Nat Methods. 2018 Dec;15(12):1108-1116.

Insert

VARNAM

Tags

Golgi and ER export signal

Expression

Mammalian

Promoter

CMV IE94

Available Since

Nov. 12, 2018

Availability

Academic Institutions and Nonprofits only

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lentiGuide-Puro PRMT5-CRISPRi

Plasmid

#164637

Purpose

lentiGuide-Puro backbone with cloned target sequence for human PRMT5. The insert is: 5´- CACCGAGCCGCGTGTCCAGCGGGA-3. sgRNA achieves a downregulation of PRMT5 in combination with dCAS9-KRAB-MCP2

Depositor

Günter Schneider

Insert

target guide sequence PRMT5 inhibition (PRMT5 Human)

Use

CRISPR and Lentiviral; Interference

Expression

Mammalian

Promoter

U6 and EF1A

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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Ii-Str_puromycin

Plasmid

#65309

Purpose

Luminal ER hook only (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Invariant chain (p33)

Expression

Mammalian

Mutation

Streptavidin in its lumenal part

Promoter

CMV

Available Since

June 19, 2015

Availability

Academic Institutions and Nonprofits only

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ii-Str_SBP-mCherry-GPI

Plasmid

#65297

Purpose

synchronize trafficking of mCherry-GPI from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

GPI anchor

Tags

IL-2 Signal Sequence and Streptavidin Binding Pro…

Expression

Mammalian

Promoter

CMV

Available Since

June 10, 2015

Availability

Academic Institutions and Nonprofits only

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pBEVY-GT

Plasmid

#51231

Purpose

bi-directional expression vector for yeast, galactose inducible, TRP1 selection

Depositor

Charles Miller

Article

Miller CA et al Nucleic Acids Res. 1998 Aug 1;26(15):357

Type

Empty backbone

Expression

Yeast

Promoter

GAL1,10

Available Since

March 21, 2014

Availability

Academic Institutions and Nonprofits only

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pBEVY-T

Plasmid

#51232

Purpose

bi-directional expression vector for yeast, constitutively active, TRP1 selection

Depositor

Charles Miller

Article

Miller CA et al Nucleic Acids Res. 1998 Aug 1;26(15):357

Type

Empty backbone

Expression

Yeast

Promoter

GPD/ADH1

Available Since

March 21, 2014

Availability

Academic Institutions and Nonprofits only

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LENG

Plasmid

#111162

Purpose

miR-E (miR-30 variant)-based RNAi with GFP reporter

Depositor

Johannes Zuber

Article

Fellmann et al Cell Rep. 2013 Dec 26;5(6):1704-13. doi:

Type

Empty backbone

Use

Retroviral

Tags

GFP

Available Since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

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pBEVY-GA

Plasmid

#51227

Purpose

bi-directional expression vector for yeast, galactose inducible, ADE2 selection

Depositor

Charles Miller

Article

Miller CA et al Nucleic Acids Res. 1998 Aug 1;26(15):357

Type

Empty backbone

Expression

Yeast

Promoter

GAL1,10

Available Since

March 21, 2014

Availability

Academic Institutions and Nonprofits only

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pEF6 Vps54-V5-6His

Plasmid

#175098

Purpose

V5 tagged Vps54 is expressed in mammalian cells

Depositor

Juan Bonifacino

Article

Perez-Victoria et al Mol Biol Cell. 2008 Jun;19(6):2350-62. d

Insert

Vps54 (Vps54 Mouse)

Tags

V5

Expression

Mammalian

Available Since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pJB37

Plasmid

#202602

Purpose

Arabinose inducible expression vector expressing retron Rcat-Sen2

Depositor

Athanasios Typas

Article

Bobonis et al Nat Protoc. 2024 May 9. doi: 10.1038/s41

Insert

RcaT-Sen2 toxin

Expression

Bacterial

Available Since

July 20, 2023

Availability

Academic Institutions and Nonprofits only

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pCS2 xRor2-mCherry

Plasmid

#67162

Purpose

mammalian expression / in vitro transcription

Depositor

Alexandra Schambony

Article

Feike et al Biochem Biophys Res Commun. 2010 Oct 1;4

Insert

Tyrosine-protein kinase transmembrane receptor ROR2 (ror2 Frog)

Use

In vitro transcription

Tags

mCherry

Expression

Mammalian

Available Since

July 16, 2015

Availability

Academic Institutions and Nonprofits only
pGEMT-T-HSV2.3 TCR alpha chain

Plasmid

#74076

Purpose

gBT-I TCR a chain cDNA in pGEM-T

Depositor

Francis R. Carbone

Article

Mueller et al Immunol Cell Biol. 2002 Apr;80(2):156-63

Insert

TCR alpha clone HSV2.3 (Tcra Mouse)

Expression

Bacterial

Available Since

March 14, 2016

Availability

Academic Institutions and Nonprofits only

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pBlueRIP.cOVA

Plasmid

#69595

Purpose

contains cytoplasmic form of ovalbumin (OVA) cDNA in pBlueRIP

Depositor

Francis R. Carbone

Article

Kurts et al J Exp Med. 1996 Sep 1;184(3):923-30.

Insert

OVA (OVAL Chicken)

Expression

Mammalian

Promoter

rat insulin promoter (RIP)

Available Since

March 7, 2016

Availability

Academic Institutions and Nonprofits only

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pCS2 xRor2-Flag

Plasmid

#67164

Purpose

mammalian expression / in vitro transcription

Depositor

Alexandra Schambony

Article

Feike et al Biochem Biophys Res Commun. 2010 Oct 1;4

Insert

Tyrosine-protein kinase transmembrane receptor ROR2 (ror2 Frog)

Use

In vitro transcription

Tags

FLag

Expression

Mammalian

Available Since

July 16, 2015

Availability

Academic Institutions and Nonprofits only
pCI-neo-AP-4 (Myc)3-σ4

Plasmid

#198331

Purpose

Expresses the triple Myc-tagged mouse AP-4 σ4 subunit in mammalian cells

Depositor

Juan Bonifacino

Article

Mattera et al Mol Biol Cell. 2022 Oct 1;33(12):ar109.

Insert

AP-4 σ4

Tags

triple Myc tag

Expression

Mammalian

Mutation

silent substitution in codon 131

Promoter

CMV

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pBlueRIP.OVA

Plasmid

#69597

Purpose

contains soluble form of ovalbumin (OVA) cDNA in pBlueRIP (used to generate RIP-OVAhi and RIP-OVA lo mice)

Depositor

Francis R. Carbone

Article

Blanas et al Science. 1996 Dec 6;274(5293):1707-9.

Insert

OVA (OVAL Chicken)

Expression

Mammalian

Promoter

rat insulin promoter (RIP)

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

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