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Plasmid#209317PurposeExpresses a sgRNA template with a protospacer cloning site flanked by BsaI restriction sites for easy insertion of a targeting cassette.DepositorInsertGroup B Streptococcus-compatible single guide RNA
UseTagsExpressionBacterialMutationPromoterXyl/tet promoter that is constitutively activeAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pVRa26_837
Plasmid#49679DepositorInsertECF26_837
UseSynthetic BiologyTagsHis6-PreScissionExpressionBacterialMutationCodon optimized for E.coliPromoterpT7-lacOAvailable sinceJan. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCAG_PCP-T5Exo
Plasmid#214736Purposeencodes T5 exonuclease with a PP7 coat protein fused to the N terminusDepositorInsertCAG_PCP-T5Exo_bGH
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceMarch 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSK+ chickBMPR-1B DN
Plasmid#49529Purposecloning vector for dominant negative chick BMP receptor 1BDepositorInsertBMPR-1B DN (BMPR1B Chicken)
UseTagsExpressionBacterialMutationdominant negative mutation (K231R); also P2A, M11…PromoterT3Available sinceMay 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
OVA-miSFIT-2xPerfect
Plasmid#124673PurposeTuning expression of cytosolic Ovalbumin coupled to EGFPDepositorInsertCyto-Ovalbumin
UseTagsExpressionMammalianMutationTruncated OVA for cytoplasmic expressionPromoterminimal CMVAvailable sinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
OVA-miSFIT-1xPerfect
Plasmid#124674PurposeTuning expression of cytosolic Ovalbumin coupled to EGFPDepositorInsertCyto-Ovalbumin
UseTagsExpressionMammalianMutationTruncated OVA for cytoplasmic expressionPromoterminimal CMVAvailable sinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLINE1ΔORF1-2-scFvFc
Plasmid#206022PurposeThe ORF1 and ORF2 sequences were removed from pLINE1-scFvFcDepositorInsertmouse LINE1 vector harboring an scFv-Fc expression unit, lacking the ORF1 and ORF2 sequences
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
HyWnt3:Luc
Plasmid#192986PurposePlasmid to test the Hydra Wnt3 promoter activityDepositorInsertHydra Wnt3 promoter
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEHlyA5
Plasmid#168298PurposeSecretion of nanobodies and other polypeptides fused to C-terminal secretion signal of HlyADepositorInsertN‑terminal His tag, VHH, HA and E‑tags, C‑HlyA
UseTagsE-tag, HA-tag, His-tag, Prescission site, and VHH…ExpressionBacterialMutationPromoterLacI-PlacAvailable sinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
HySp5-310
Plasmid#192995PurposePlasmid that encodes for Hydra Sp5, lacking the SP boxDepositorInsertHydra Sp5
UseTagsHAExpressionMammalianMutationno SP boxPromoterAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEM-T-Easy-HySp5-557
Plasmid#193000PurposePlasmid to generate an ISH probe for Hydra Sp5DepositorInsertHydra Sp5
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
HySp5-227
Plasmid#192996PurposePlasmid that encodes for Hydra Sp5, lacking the SP box and DNA binding domainDepositorInsertHydra Sp5
UseTagsHAExpressionMammalianMutationno SP box and DNA binding domainPromoterAvailable sinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
HySp5:Luc
Plasmid#192985PurposePlasmid to test the Hydra Sp5 promoter activityDepositorInsertHydra Sp5 promoter
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
HySp5-337
Plasmid#192994PurposePlasmid that encodes for Hydra Sp5, lacking the DNA binding domainDepositorInsertHydra Sp5
UseTagsHAExpressionMammalianMutationno DNA binding domainPromoterAvailable sinceDec. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
ZfWnt3:Luc
Plasmid#192992PurposePlasmid to test the Zebrafish Wnt3 promoter activityDepositorInsertZebrafish Wnt3 promoter
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAGM9121_Gma-UPO SP
Plasmid#153495PurposeGalerina marginata Unspecific Peroxygenase Signal Peptide Donor PlasmidDepositorInsertUnspecific peroxygenase SP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
loxP-myctagBFP-STOP-loxP-mCherry-NTR
Plasmid#108456PurposeThis plasmid can be used to recombine the loxP-myctagBFP-STOP-loxP-mCherry-NTR cassette (or only mCherry-NTR) into a BACDepositorInsertloxP-myctagBFP-STOP-loxP-mCherry-NTR
UseCre/LoxTagsExpressionMutationPromoterAvailable sinceJune 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLAS2w.Phyg-CHST8
Plasmid#232650Purposeexpresses CHST8 wild-type in mammalian cellsDepositorInsertCHST8 (CHST8 Human)
UseLentiviralTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceMarch 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLINE1mORF1-ΔORF2-scFvFc
Plasmid#206023PurposeThe base next to the ORF1 start codon (ATG) was deleted in pLINE1ΔORF2-scFvFcDepositorInsertmouse LINE1 vector harboring an scFv-Fc expression unit, encoding mutated ORF1 but lacking ORF2
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRRG227-Wnt1
Plasmid#99073PurposedsRNA and riboprobe synthesis for Schmidtea mediterranea Wnt1 (also known as WntP-1)DepositorInsertWnt1 (also known as WntP-1) in situ probe
UseT/a cloning vector for dsrna generation and the g…TagsExpressionMutationPromoterAvailable sinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only