We narrowed to 5,835 results for: pCas

1. pCA528 CAPN7 (1-75)

   Plasmid

   #193031

   Purpose

   Bacterial expression plasmid for CAPN7 (1-75) N-terminal MIT domain

   Depositor

   Wesley Sundquist

   Article

   Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

   Insert

   CAPN7 (CAPN7 Human)
   

   Tags

   6xHis-Sumo

   Expression

   Bacterial

   Mutation

   Residues 1-75

   Promoter

   T7

   Available Since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. SpCas9-miniCGBE1

   Plasmid

   #198548

   Purpose

   Expresses human codon-optimized SpCas9-miniCGBE1 and blasticidin resistance: EFS promoter-SpCas9-miniCGBE1-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

   Insert

   SpCas9-miniCGBE1
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Promoter

   EFS

   Available Since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. SpCas9-NG-miniCGBE1

   Plasmid

   #198538

   Purpose

   Expresses human codon-optimized SpCas9-NG-miniCGBE1 and blasticidin resistance: EFS promoter-SpCas9-NG-miniCGBE1-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

   Insert

   SpCas9-NG-miniCGBE1
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Available Since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. SpCas9-NRRH

   Plasmid

   #198540

   Purpose

   Expresses human codon-optimized SpCas9-NRRH and blasticidin resistance: EFS promoter-SpCas9-NRRH-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

   Insert

   SpCas9-NRRH
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Promoter

   EFS

   Available Since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. SpCas9-NRCH

   Plasmid

   #198542

   Purpose

   Expresses human codon-optimized SpCas9-NRCH and blasticidin resistance: EFS promoter-SpCas9-NRCH-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

   Insert

   SpCas9-NRCH
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Promoter

   EFS

   Available Since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCas3-Apr

   Plasmid

   #208000

   Purpose

   Expresses all necessary components of Type I-C CRISPR-Cas system

   Depositor

   Pablo Ivan Nikel

   Article

   Lammens et al Microbiol Spectr. 2023 Nov 17:e0270723.

   Inserts

   RhaR (rhaR )
   RhaS (rhaS )
   pRhaBAD
   I-C crRNA
   Cas3
   Cas5
   Cas8
   

   Expression

   Mammalian

   Available Since

   Nov. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG_SRGAP2A_GAP(R527L_K566A)

   Plasmid

   #222617

   Purpose

   Expresses full length SRGAP2 with R527L, K566A mt in the Rac1-specific GAP domain that abrogates the GTPase activating function of SRGAP2 without locking the GAP-domain in a form bound to Rac1-GDP.

   Depositor

   Franck Polleux

   Article

   Fossati et al Neuron. 2016 Jul 20;91(2):356-69. doi: 1

   Insert

   SRGAP2 (SRGAP2 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Point mutations R527L and K566A (Rac1-specific GA…

   Available Since

   Oct. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSAVED-CHAT_PCaspase

   Plasmid

   #214043

   Purpose

   Bacterial expression plasmid for SAVED-CHAT and PCaspase N-terminal fragment (aa 1-153) from Haliangium ochraceum

   Depositor

   Raymond Staals

   Article

   Steens et al Science. 2024 Feb 2;383(6682):512-519. d

   Insert

   SAVED-CHAT
   

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   lacUV5 promoter

   Available Since

   June 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSAVED-CHAT_PCaspase_PCi

   Plasmid

   #214049

   Purpose

   Bacterial expression plasmid for SAVED-CHAT, PCaspase, and PCi from Haliangium ochraceum

   Depositor

   Raymond Staals

   Article

   Steens et al Science. 2024 Feb 2;383(6682):512-519. d

   Insert

   SAVED-CHAT-Pcaspase-PCi
   

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   lacUV5 promoter

   Available Since

   June 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAT.482

    Plasmid

    #203952

    Purpose

    Spectinomycin-based RSF1010 acceptor Level T

    Depositor

    Alistair McCormick

    Article

    Victoria et al Plant Physiol. 2024 May 7:kiae261. doi:

    Type

    Empty backbone

    Use

    Synthetic Biology

    Available Since

    May 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG_ALX4-Flag-HA

    Plasmid

    #215028

    Purpose

    Mammalian expression of human ALX4

    Depositor

    Joanna Wysocka

    Article

    Kim et al Cell. 2024 Feb 1;187(3):692-711.e26. doi

    Insert

    ALX homeobox 4 (ALX4 Human)
    

    Tags

    Flag-HA

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-ORF3cStrep

    Plasmid

    #208728

    Purpose

    Expresses ORF3c-Strep in mammalian cells

    Depositor

    Andrew Firth

    Insert

    ORF3c (ORF3a )
    

    Tags

    Strep

    Expression

    Mammalian

    Available Since

    Feb. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG-ORF3cHA

    Plasmid

    #208653

    Purpose

    Express ORF3c-HA in mammalian cells

    Depositor

    Andrew Firth

    Insert

    ORF3c (ORF3a )
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Feb. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAB1-YFP-GW

    Plasmid

    #209363

    Purpose

    Gateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis CAB1 promoter driving expression of N-terminal YFP fusion protein.

    Depositor

    Lucia Strader

    Article

    Michniewicz et al BMC Res Notes. 2015 Mar 3;8:63. doi: 10.

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    Jan. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGL0_156 [PcatP]

    Plasmid

    #210678

    Purpose

    Level 0 part

    Depositor

    Charlie Gilbert, Cultivarium Tools, Nili Ostrov

    Insert

    PcatP
    

    Use

    Synthetic Biology

    Available Since

    Jan. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. LLP781_SnoopCatcher-KRAB-CO

    Plasmid

    #211774

    Purpose

    SnoopTag counterpart binding domain, SnoopCatcher, fused to transcriptional repressor KRAB, with GFP selection

    Depositor

    Ryan Lister

    Article

    Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

    Insert

    Traptavidin-KRAB
    

    Tags

    3xV5

    Expression

    Mammalian

    Mutation

    Last 300 bp codon-optimised (CO) to detect KRAB e…

    Promoter

    pEF1a and pSV40

    Available Since

    Dec. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG/ATP11C-c-HA

    Plasmid

    #209222

    Purpose

    Mammalian expression of ATP11C isoform c

    Depositor

    Hye-Won Shin

    Article

    Inoue et al J Cell Sci. 2021 Oct 15;134(20):jcs25852

    Insert

    ATP11C isoform c (ATP11C Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG/ATP11C-b-HA

    Plasmid

    #209221

    Purpose

    Mammalian expression of ATP11C isoform b

    Depositor

    Hye-Won Shin

    Article

    Inoue et al J Cell Sci. 2021 Oct 15;134(20):jcs25852

    Insert

    ATP11C isoform b (ATP11C Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. Cav2.2 pCAGGS

    Plasmid

    #206101

    Purpose

    expression of rabbit Cav2.2 calcium channel, the 3'UTR is deleted, this enhances the expression

    Depositor

    Annette Dolphin

    Article

    Page et al J Neurosci. 2004 Jun 9;24(23):5400-9. do

    Insert

    cacna1b (CACNA1B Rabbit)
    

    Expression

    Mammalian

    Promoter

    CMV/B-actin

    Available Since

    Nov. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAG/ATP8B3-HA

    Plasmid

    #209211

    Purpose

    Mammalian expression of ATP8B3

    Depositor

    Hye-Won Shin

    Article

    Takatsu et al J Biol Chem. 2011 Nov 4;286(44):38159-38

    Insert

    ATP8B3 (ATP8B3 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Oct. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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