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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 5,900 results for: pCas

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Showing: 821 - 840 of 5900 results

  4. pCas3-APOBEC1

    Plasmid
    #229536
    Purpose
    pMV_hyg encoding Cas3(wt)-rAPOBEC1 fusion construct and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloning
    Depositor
    Kevin Verstrepen
    Article
    Zimmermann et al Nat Commun. 2023 Jun 9;14(1):3389. doi:
    Inserts
    rAPOBEC1
    Cas3
    Uracil glycosylase inhibitor
    Tags
    XTEN
    Expression
    Bacterial and Yeast
    Mutation
    codon optimized for S.cerevisiae and codon optimi…
    Available Since
    Jan. 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    229536-plasmid-map-sequence-id-458911

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  5. pCas3-CDA1

    Plasmid
    #229534
    Purpose
    pMV_hyg encoding Cas3(wt)-CDA1 fusion construct and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloning
    Depositor
    Kevin Verstrepen
    Article
    Zimmermann et al Nat Commun. 2023 Jun 9;14(1):3389. doi:
    Inserts
    Cas3
    Cytidine deaminase
    Uracil glycosylase inhibitor
    Tags
    XTEN
    Expression
    Bacterial and Yeast
    Mutation
    codon optimized for S.cerevisiae and codon optimi…
    Available Since
    Jan. 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    229534-plasmid-map-sequence-id-458891

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  9. SpCas9-miniCGBE1

    Plasmid
    #198548
    Purpose
    Expresses human codon-optimized SpCas9-miniCGBE1 and blasticidin resistance: EFS promoter-SpCas9-miniCGBE1-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.
    Insert
    SpCas9-miniCGBE1
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Promoter
    EFS
    Available Since
    Nov. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    198548-plasmid-map-sequence-id-388891

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  11. SpCas9-NRRH

    Plasmid
    #198540
    Purpose
    Expresses human codon-optimized SpCas9-NRRH and blasticidin resistance: EFS promoter-SpCas9-NRRH-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.
    Insert
    SpCas9-NRRH
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Promoter
    EFS
    Available Since
    Nov. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    198540-plasmid-map-sequence-id-388897

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  12. SpCas9-NRCH

    Plasmid
    #198542
    Purpose
    Expresses human codon-optimized SpCas9-NRCH and blasticidin resistance: EFS promoter-SpCas9-NRCH-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.
    Insert
    SpCas9-NRCH
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Promoter
    EFS
    Available Since
    Nov. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    198542-plasmid-map-sequence-id-388906

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  14. pCAG_SRGAP2A_GAP(R527L_K566A)

    Plasmid
    #222617
    Purpose
    Expresses full length SRGAP2 with R527L, K566A mt in the Rac1-specific GAP domain that abrogates the GTPase activating function of SRGAP2 without locking the GAP-domain in a form bound to Rac1-GDP.
    Depositor
    Franck Polleux
    Article
    Fossati et al Neuron. 2016 Jul 20;91(2):356-69. doi: 1
    Insert
    SRGAP2 (SRGAP2 Human)
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Point mutations R527L and K566A (Rac1-specific GA…
    Available Since
    Oct. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    222617-plasmid-map-sequence-id-448408

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  19. pCAG-ORF3cStrep

    Plasmid
    #208728
    Purpose
    Expresses ORF3c-Strep in mammalian cells
    Depositor
    Andrew Firth
    Insert
    ORF3c (ORF3a )
    Tags
    Strep
    Expression
    Mammalian
    Available Since
    Feb. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    208728-plasmid-map-sequence-id-420909

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  20. pCAG-ORF3cHA

    Plasmid
    #208653
    Purpose
    Express ORF3c-HA in mammalian cells
    Depositor
    Andrew Firth
    Insert
    ORF3c (ORF3a )
    Tags
    HA
    Expression
    Mammalian
    Available Since
    Feb. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    208653-plasmid-map-sequence-id-420908

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Showing: 821 - 840 of 5900 results