We narrowed to 3,239 results for: pEGFP

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pEGFP-N2-2XNLS-RNaseH1 delta 1-27 (WT)

Plasmid

#196702

Purpose

Plasmid for transient mammalian expression of wild type RNase H1 tagged with 2xNLS and EGFP that can be used to specifically degrade the nuclear R-loops.

Depositor

Karlene Cimprich

Article

Crossley et al Nature. 2023 Jan;613(7942):187-194. doi:

Insert

human RNaseH1 wild type

Expression

Mammalian

Available Since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-MRLC1 T18D, S19D

Plasmid

#35682

Depositor

Tom Egelhoff

Article

Beach et al BMC Cell Biol. 2011 Dec 2;12:52.

Insert

MRLC1 (MYL9 Human)

Tags

EGFP

Expression

Mammalian

Mutation

T18D and S19D

Promoter

CMV

Available Since

April 24, 2012

Availability

Academic Institutions and Nonprofits only
pEGFP-MRLC T18A,S19A

Plasmid

#35681

Depositor

Tom Egelhoff

Article

Beach et al BMC Cell Biol. 2011 Dec 2;12:52.

Insert

MRLC1 (MYL9 Human)

Tags

EGFP

Expression

Mammalian

Mutation

T18A and S19A

Promoter

CMV

Available Since

April 24, 2012

Availability

Academic Institutions and Nonprofits only
pEGFP-h53BP1 (siRNA resistant)

Plasmid

#110301

Purpose

Mammalian expression of a EGFP-tagged full length human 53BP1 (resistant to siRNA targeting AGAACGAGGAGACGGTAATAGTGGG)

Depositor

Chris Kok-Lung Chan

Article

Tiwari et al Nat Commun. 2018 Feb 14;9(1):677. doi: 1

Insert

p53-binding protein 1 (TP53BP1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

GAACGAGGA to GAGCGGGGC

Available Since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP_mNG2(11)_Clathrin light chain

Plasmid

#82608

Purpose

Expresses mNG2(11) tagged clathrin light chain in mammalian cells

Depositor

Bo Huang

Article

Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

Insert

mNG2(11)_Clathrin light chain

Expression

Mammalian

Available Since

May 15, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-hsRPA70 (MSW#473)

Plasmid

#208067

Purpose

Expression of GFP-tagged hsRPA1 in human cells

Depositor

Marc Wold

Article

Haring et al J Biol Chem. 2008 Jul 4;283(27):19095-11

Insert

RPA1 (RPA1 Human)

Tags

GFP

Expression

Mammalian

Available Since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-hsRPA32 (MSW#497)

Plasmid

#208068

Purpose

Expression of GFP-tagged hsRPA2 in human cells

Depositor

Marc Wold

Article

Haring et al J Biol Chem. 2008 Jul 4;283(27):19095-11

Insert

RPA2 (RPA2 Human)

Tags

GFP

Expression

Mammalian

Mutation

T88I- please see depositor comments

Available Since

Dec. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-hsRPA14 (MSW#503)

Plasmid

#208069

Purpose

Expression of GFP-tagged hsRPA3 in human cells

Depositor

Marc Wold

Article

Haring et al J Biol Chem. 2008 Jul 4;283(27):19095-11

Insert

RPA3 (RPA3 Human)

Tags

GFP

Expression

Mammalian

Available Since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFPC1-FLAG-Synaptojanin 1-145

Plasmid

#22293

Depositor

Pietro De Camilli

Article

Perera et al Proc Natl Acad Sci U S A. 2006 Dec 19. 1

Insert

Synaptojanin 1_145 (SYNJ1 Human)

Tags

Flag and GFP

Expression

Bacterial and Mammalian

Available Since

Oct. 21, 2009

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-FUS/TLS-FLAGC

Plasmid

#60362

Purpose

Plasmid for expression of FLAG-GFP tagged human FUS/TLS (C-terminal tag). Confers resistance to G418.

Depositor

Sébastien Britton, Patrick Calsou

Article

Britton et al Nucleic Acids Res. 2014 Oct 1;42(14):904

Insert

FUS (FUS Human)

Tags

FLAG and GFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 8, 2015

Availability

Academic Institutions and Nonprofits only

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pEGFP Centrin2 (Nigg UK185)

Plasmid

#41147

Depositor

Erich Nigg

Article

Kleylein-Sohn et al Dev Cell. 2007 Aug;13(2):190-202.

Insert

Centrin-2 (CETN2 Human)

Tags

eGFP

Expression

Mammalian

Promoter

CMV

Available Since

June 7, 2013

Availability

Academic Institutions and Nonprofits only

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pEGFP-C2-MVP delta607-623aa

Plasmid

#204544

Purpose

Expresses EGFP-tagged MVP delta607-623aa in mammalian cells

Depositor

Masaaki Komatsu

Article

Kurusu et al Dev Cell. 2023 May 9:S1534-5807(23)00191

Insert

MVP (MVP Human)

Expression

Mammalian

Mutation

delta607-623aa

Available Since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-TetR-NLS-VP16

Plasmid

#103834

Purpose

Transcription activator (transactivation domain of viral VP16 protein) that is constitutively recruited tetO arrays

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

EGFP-TetR-NLS-VP16 (tetR )

Expression

Mammalian

Promoter

SV2

Available Since

Dec. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-phSyn-flex-SypEGFP

Plasmid

#153203

Purpose

Can be used to generate AAV virus that will mark the presynaptic terminal (synaptophysin-fused) EGFP in the presence of Cre in neurons from the synapsin promoter

Depositor

Lynette Lim

Insert

synaptophysin-EGFP

Use

AAV

Promoter

rat synapsin

Available Since

June 2, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1 Lifeact-EGFP-2XNLS

Plasmid

#58467

Purpose

Expresses a nuclear-targeted actin filament reporter, consisting of the peptide Lifeact, on a CMV promoter

Depositor

Dyche Mullins

Article

Belin et al Mol Biol Cell. 2013 Apr;24(7):982-94. do

Inserts

Lifeact
2X NLS with 2X stop codons

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

April 26, 2016

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1X-Nup93-mut

Plasmid

#87335

Purpose

To express human Nup93 in mammalian cells. It contains synonymous mutations that are not targeted by shRNA

Depositor

Lei Lu

Article

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

NUP93 (NUP93 Human)

Tags

EGFP

Expression

Mammalian

Mutation

shRNA resistant changes

Promoter

CMV

Available Since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1 hMOG-alpha1-EGFP

Plasmid

#126463

Purpose

Expression a human MOG (isoform alpha1) EGFP fusion protein in mammalian cells

Depositor

Markus Reindl

Article

Mader et al J Neuroinflammation. 2011 Dec 28;8:184.

Insert

Myelin oligodendrocyte glycoprotein (MOG Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 28, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1 human cofilin S3E

Plasmid

#50861

Purpose

expresses psuedo-phosporylated, non-activatable cofilin fused to EGFP in mammalian cells

Depositor

James Bamburg

Article

Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.

Insert

cofilin 1 S3E (CFL1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

S3E psuedo-phosporylated, non-activatable

Promoter

CMV

Available Since

Feb. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1 Utr230-EGFP-3XNLS

Plasmid

#58466

Purpose

Expresses a nuclear-targeted actin filament reporter, consisting of the first 230 amino acids of human utrophin, on a CMV promoter

Depositor

Dyche Mullins

Article

Belin et al Mol Biol Cell. 2013 Apr;24(7):982-94. do

Inserts

Utr230 with 3X glycine linker (UTRN Human)
3X NLS with 3X stop codons

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1 human cofilin S3A

Plasmid

#50860

Purpose

expresses constituvely active cofilin fused to EGFP in mammalian cells

Depositor

James Bamburg

Article

Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.

Insert

cofilin 1 S3A (CFL1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

S3A constitutively active

Promoter

CMV

Available Since

Feb. 5, 2014

Availability

Academic Institutions and Nonprofits only

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