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pRL542

Plasmid

#70282

Purpose

ColK-based vector; provides a nicking function to permit conjugal transfer and bears a polylinker for insertion of methylase-encoding genes

Depositor

Peter Wolk

Article

Elhai et al J Bacteriol. 1997 Mar;179(6):1998-2005.

Type

Empty backbone

Expression

Bacterial

Available Since

Feb. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV-MBP-yATOM_mCherry

Plasmid

#209707

Purpose

Mammalian expression of y-ATOM biosensor for mCherry

Depositor

Stewart Loh

Article

Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

Insert

MBP-yATOM_mCherry

Tags

MBP

Expression

Mammalian

Available Since

Nov. 13, 2023

Availability

Academic Institutions and Nonprofits only

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lacI-Ptac-lacO-BsaI-BsaI-IMBB4-pUC

Plasmid

#170772

Purpose

test plasmid for Golden Gate assembly, expresses lacI repressor

Depositor

Ichiro Matsumura

Article

Matsumura et al Biotechniques. 2022 May;72(5):185-193. d

Insert

lacI

Promoter

lacI promoter

Available Since

Feb. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pRRL.SP107.gp47.eGFP

Plasmid

#30472

Depositor

Didier Trono

Article

Barde et al Gene Ther. 2011 Nov;18(11):1087-97. doi:

Insert

SP107/gp47/eGFP

Use

Lentiviral

Available Since

Aug. 24, 2011

Availability

Academic Institutions and Nonprofits only

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pCMV-MBP-yATOM_Ras

Plasmid

#209709

Purpose

Mammalian expression of y-ATOM biosensor for HRas

Depositor

Stewart Loh

Article

Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

Insert

MBP-yATOM_Ras

Tags

MBP

Expression

Mammalian

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pDEST-APIGH

Plasmid

#112804

Purpose

gateway cloning destination vector to express enhancer driven Gal4 in Drosophila

Depositor

Chun Han, Yuh-Nung Jan

Article

Han et al Neuron. 2012 Jan 12;73(1):64-78. doi: 10

Type

Empty backbone

Expression

Bacterial

Available Since

Sept. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pLV.SP146.gp91.GP91.cHS4

Plasmid

#30480

Depositor

Didier Trono

Article

Barde et al Gene Ther. 2011 Nov;18(11):1087-97. doi:

Insert

SP146/gp91/GP91/cHS4

Use

Lentiviral

Available Since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only

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pMXLC_pFa

Plasmid

#114219

Purpose

POC12355, MetClo assembly vector with p15A replication origin and chloramphenicol resistance for BsaI-based MetClo, adaptor sequence type p-a

Depositor

Christopher A. O'Callaghan

Article

Lin et al Nucleic Acids Res. 2018 Jul 9. pii: 5050

Insert

LacZalpha

Use

Synthetic Biology

Available Since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pMXLK_dFq

Plasmid

#114237

Purpose

POC12377, MetClo assembly vector with p15A replication origin and kanamycin resistance for BsaI-based MetClo, adaptor sequence type d-q

Depositor

Christopher A. O'Callaghan

Article

Lin et al Nucleic Acids Res. 2018 Jul 9. pii: 5050

Insert

LacZalpha

Use

Synthetic Biology

Available Since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pMXLK_pFa

Plasmid

#114229

Purpose

POC12367, MetClo assembly vector with p15A replication origin and kanamycin resistance for BsaI-based MetClo, adaptor sequence type p-a

Depositor

Christopher A. O'Callaghan

Article

Lin et al Nucleic Acids Res. 2018 Jul 9. pii: 5050

Insert

LacZalpha

Use

Synthetic Biology

Available Since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pLV.CMVenh.gp91.GP91.cHS4

Plasmid

#30479

Depositor

Didier Trono

Article

Barde et al Gene Ther. 2011 Nov;18(11):1087-97. doi:

Insert

CMVenh/gp91/GP91/cHS4

Use

Lentiviral

Available Since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only

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pRRL.SP107.gp91.eGFP

Plasmid

#30473

Depositor

Didier Trono

Article

Barde et al Gene Ther. 2011 Nov;18(11):1087-97. doi:

Insert

SP107/gp91/eGFP

Use

Lentiviral

Available Since

Aug. 24, 2011

Availability

Academic Institutions and Nonprofits only

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pMXLP_pFa

Plasmid

#114239

Purpose

POC12379, MetClo assembly vector with p15A replication origin and ampicillin resistance for BsaI-based MetClo, adaptor sequence type p-a

Depositor

Christopher A. O'Callaghan

Article

Lin et al Nucleic Acids Res. 2018 Jul 9. pii: 5050

Insert

LacZalpha

Use

Synthetic Biology

Available Since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only

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POC1468

Plasmid

#213756

Purpose

BsaI-associated non-switchable methylase

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Appl Microbiol Biotechnol. 2024 Jan 25;1

Insert

Non-switchable methylase M2.BsaI

Tags

His6-tag

Expression

Bacterial

Promoter

T7

Available Since

July 17, 2024

Availability

Academic Institutions and Nonprofits only

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POC1467

Plasmid

#213755

Purpose

BsaI-associated non-switchable methylase

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Appl Microbiol Biotechnol. 2024 Jan 25;1

Insert

Non-switchable methylase M2.BsaI

Tags

His6-tag

Expression

Bacterial

Promoter

T7

Available Since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-RBP-WDR5

Plasmid

#209702

Purpose

Mammalian expression of WDR5 with N terminal RBP fusion

Depositor

Stewart Loh

Article

Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

Insert

RBP-WDR5

Tags

RBP

Expression

Mammalian

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-Cox4-Clover

Plasmid

#209721

Purpose

Mammalian expression of clover localized to mitochondria

Depositor

Stewart Loh

Article

Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

Insert

Cox4-Clover

Tags

Cos4

Expression

Mammalian

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-MBP-yATOM_WDR5

Plasmid

#209706

Purpose

Mammalian expression of y-ATOM biosensor for WDR5

Depositor

Stewart Loh

Article

Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

Insert

MBP-yATOM_WDR5

Tags

MBP

Expression

Mammalian

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-MBP-yATOM_SH2

Plasmid

#209708

Purpose

Mammalian expression of y-ATOM biosensor for SH2

Depositor

Stewart Loh

Article

Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

Insert

MBP-yATOM_SH2

Tags

MBP

Expression

Mammalian

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-Cox4-SH2

Plasmid

#209717

Purpose

Mammalian expression of SH2 localized to mitochondria

Depositor

Stewart Loh

Article

Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

Insert

Cox4-SH2

Tags

Cos4

Expression

Mammalian

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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