We narrowed to 10,386 results for: Coli
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Plasmid#131379PurposeLow level expression of E.coli G.stearothermophillus MutY chimera protein with amino acid changes F307A and S308A in Gs MutY.DepositorInsertEcNGsC MutY chimera F307A S308A
ExpressionBacterialMutationFusion of two MutY genes. Residues 1-225 are fro…PromotertacAvailable SinceJan. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
EcNGsC-F307Y-pKK223
Plasmid#131381PurposeLow level expression of E.coli G.stearothermophillus MutY chimera protein with amino acid change F307Y in Gs MutY.DepositorInsertEcNGsC MutY chimera F307Y
ExpressionBacterialMutationFusion of two MutY genes. Residues 1-225 are from…PromotertacAvailable SinceJan. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
EcNGsC-F307A-pKK223
Plasmid#131382PurposeLow level expression of E.coli G.stearothermophillus MutY chimera protein with amino acid change F307A in Gs MutY.DepositorInsertEcNGsC MutY chimera F307A
ExpressionBacterialMutationFusion of two MutY genes. Residues 1-225 are from…PromotertacAvailable SinceJan. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
EcNGsC-S308V-pKK223
Plasmid#131393PurposeLow level expression of E.coli G.stearothermophillus MutY chimera protein with amino acid change S308V in Gs MutY.DepositorInsertEcNGsC MutY chimera S308V
ExpressionBacterialMutationFusion of two MutY genes. Residues 1-225 are fro…PromotertacAvailable SinceJan. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
EcNGsC-S308A-pKK223
Plasmid#131394PurposeLow level expression of E.coli G.stearothermophillus MutY chimera protein with amino acid change S308A in Gs MutY.DepositorInsertEcNGsC MutY chimera S308A
ExpressionBacterialMutationFusion of two MutY genes. Residues 1-225 are fro…PromotertacAvailable SinceJan. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET29a-hNABP1NhNABP2C
Plasmid#128312PurposeExpress chimeric protein hNABP1 N-terminus and hNABP2 C-terminus in E. coli with a C-terminal His-tagDepositorTagsHisExpressionBacterialMutationchimeric protein hNABP1N+hNABP2CPromoterT7Available SinceNov. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET29a-hNABP1-N
Plasmid#128310PurposeExpress hNABP1 N-terminus (1–141 aa) in E. coli with a C-terminal His-tagDepositorInserthNABP1 N-terminal domain (OB fold, 1–141 aa) (NABP1 Human)
TagsHisExpressionBacterialMutationN-terminal domain (OB fold, 1–140 aa)PromoterT7Available SinceAug. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET29a-hNABP2-C
Plasmid#128309PurposeExpress hNABP2 C-terminus(122–211 aa) in E. coli with a C-terminal His-tagDepositorInserthNABP2 C-terminal domain (122–211 aa) (NABP2 Human)
TagsHisExpressionBacterialMutationC-terminal domain (122–211 aa)PromoterT7Available SinceAug. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSR357.12
Plasmid#125107PurposeExpression of narL(1-134aa)-ydfI(129-213aa) chimera under PLtetO-1, output Promoter PydfJ115DepositorInsertsnarL(REC)-ydfI(DBD)134
sfgfp
tetR
UseSynthetic BiologyExpressionBacterialMutationA84T - see depositor comments below and Q183K-see…Available SinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET-DEST42-ARL4D-V5-His6
Plasmid#67426PurposeExpression of hArl4D with C-term V5 and His6 tag in E. coliDepositorInsertARL4D (ARL4D Human)
TagsHis and V5ExpressionBacterialMutationbp 272 A to C; N91T mutation; bp 327 T to G; sil…PromoterT7/lacOAvailable SinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28-p53 (1-73) (K24N)
Plasmid#62079PurposeExpression of human p53 (1-73) (K24N) in e. coliDepositorInsertp53 (TP53 Human)
TagsHisExpressionBacterialMutationcontains TAD residues 1-73; K24NPromoterT7Available SinceJan. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
hSRAP.TWIN1.a111-215
Plasmid#49820Purposeexpression vector for SRAP(111-215) in pTWIN1 vectorDepositorInsertsteroid receptor RNA activator protein aa 111-215 (SRA1 Human)
Tagsintein & chitin binding domainExpressionBacterialMutationwildtype codon-optimized for E. coli expressionPromoterT7Available SinceJan. 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
hSRAP.TWIN1.a105-236.V105M
Plasmid#49819Purposeexpression vector for SRAP(105-236)V105M in pTWIN1 vectorDepositorInsertsteroid receptor RNA activator protein aa 105-236 (SRA1 Human)
Tagsintein & chitin binding domainExpressionBacterialMutationV105M codon-optimized for E. coli expressionPromoterT7Available SinceJan. 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
hSRAP.TWIN1.a105-215.V105M
Plasmid#49818Purposeexpression vector for SRAP(105-215)V105M in pTWIN1 vectorDepositorInsertsteroid receptor RNA activator protein aa 105-215 (SRA1 Human)
Tagsintein & chitin binding domainExpressionBacterialMutationV105M codon-optimized for E. coli expressionPromoterT7Available SinceJan. 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28b-irp3 WT
Plasmid#87106Purposeexpresses Irp3 WT, the thiazolinyl imine reductase from Yersinia enterocoliticaDepositorInsertirp3 WT
Tagshis-tagExpressionBacterialPromoterT7Available SinceMarch 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBCSK_HIS3_II
Plasmid#109059Purposeyeast shuttle vector with deletable CEN6-ARSH4 cassette. For teaching purposes.DepositorInsertHIS3 (HIS3 Zygosaccharomyces rouxii, Budding Yeast)
UseCre/LoxExpressionBacterial and YeastPromoterHIS3Available SinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBCSK_HIS3_I
Plasmid#109058Purposeyeast shuttle vector with deletable CEN6-ARSH4 cassette. For teaching purposes.DepositorInsertHIS3 (HIS3 Zygosaccharomyces rouxii, Budding Yeast)
UseCre/LoxExpressionBacterial and YeastPromoterHIS3Available SinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBCSK_LEU2_I
Plasmid#109060Purposeyeast shuttle vector for insertion into yeast chromosomes in pBCSK backbone. LoxP sites upstream of LEU2. For teaching purposesDepositorInsertLEU2 (LEU2 Zygosaccharomyces rouxii, Budding Yeast)
UseCre/LoxExpressionBacterial and YeastPromoterLEU2Available SinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBCSK_LEU2_II
Plasmid#109061Purposeyeast shuttle vector for insertion into yeast chromosomes in pBCSK backbone. LoxP sites downstream of LEU2. For teaching purposes.DepositorInsertLEU2 (LEU2 Zygosaccharomyces rouxii, Budding Yeast)
UseCre/LoxExpressionBacterial and YeastPromoterLEU2Available SinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBSIIKS_HIS3_I
Plasmid#109062Purposeyeast shuttle vector with deletable CEN6-ARSH4 cassette in pBIIKS backbone. LoxP site upstream of HIS3. For teaching purposes.DepositorInsertHIS3 (HIS3 Zygosaccharomyces rouxii, Budding Yeast)
UseCre/LoxExpressionBacterial and YeastPromoterHIS3Available SinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only