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CL20_mEGFP_PTEN_RPL11

Plasmid

#205903

Purpose

Express mEGFP-tagged fusion protein, PTEN_RPL11 from patient-derived sequence

Depositor

Richard Kriwacki

Publication

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PTEN_RPL11 (PTEN, RPL11 Human)

Tags

mEGFP

Expression

Mammalian

Available since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pFH2.97_PB-EPN1-MCP-T2A-eGFP-PGK-Blast

Plasmid

#205542

Purpose

Express EPN1-MCP-T2A-eGFP and blasticidin resistance marker

Depositor

Michael Elowitz

Publication

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

EPN1-MCP

Use

Piggybac

Expression

Mammalian

Mutation

WT

Promoter

EF1

Available since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pFH2.102_PB-EPN11-MCP_I-T2A-eGFP-PGK-Blast

Plasmid

#205547

Purpose

Express EPN11-MCP-I-T2A-eGFP and blasticidin resistance marker

Depositor

Michael Elowitz

Publication

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

EPN11-MCP-I

Use

Piggybac

Expression

Mammalian

Mutation

WT

Promoter

EF1

Available since

Sept. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pGL3-MTS-CCDB-N136-RsDddA-A-UGI-PURO

Plasmid

#205463

Purpose

A backbone containing a RsDddA fragment A for constructing mitochondria-targeted RsDdCBE expression plasmid

Depositor

Bin Shen

Publication

Cheng et al Mol Ther Nucleic Acids. 2023 Sep 9;34:10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

MTS; N136; ccdb; RsDddA-A; UGI

Expression

Mammalian

Available since

Aug. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pGL3-MTS-CCDB-N136-RsDddA-C-UGI-PURO

Plasmid

#205465

Purpose

A backbone containing a RsDddA fragment C for constructing mitochondria-targeted RsDdCBE expression plasmid

Depositor

Bin Shen

Publication

Cheng et al Mol Ther Nucleic Acids. 2023 Sep 9;34:10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

MTS; N136; ccdb; RsDddA-C; UGI

Expression

Mammalian

Available since

Aug. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pJPT003-PM-HaloTag-1x FLAG

Plasmid

#202494

Purpose

Express "PM" HaloTag

Depositor

Neha Kamat

Publication

Peruzzi et al Nat Commun. 2024 Jul 4;15(1):5618. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

HaloTag

Tags

1x FLAG and Palmitoylation-Myristoylation Site

Expression

Mammalian

Promoter

CMV

Available since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-KIR3DX1-COMP5AP-AviTag-9xHis

Plasmid

#157338

Purpose

Mammalian expression of secreted protein fused to COMP5AP-AviTag-9xHis.

Depositor

Chris Garcia

Publication

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

KIR3DX1 (KIR3DX1 Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsXRN1-del1174-1649_AH

Plasmid

#148904

Purpose

Mammalian Expression of HsXRN1-del1174-1649

Depositor

Elisa Izaurralde

Publication

Chang et al Nucleic Acids Res. 2019 Sep 26;47(17):92

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

HsXRN1-del1174-1649 (XRN1 Human)

Expression

Mammalian

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pDF0385 pcmv-hudiscas7-11-r1045-gggs-r1122

Plasmid

#186982

Purpose

mammalian expression of cas7-11-r1045-gggs-r1122

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Publication

Kato et al Cell. 2022 May 25. pii: S0092-8674(22)00

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

cas7-11-r1045-gggs-r1122

Expression

Mammalian

Mutation

r1045-gggs-r1122 deletion

Available since

Aug. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pIG-746pUC19-tNGFR-P2A-CD5

Plasmid

#186121

Purpose

Knockin of truncated NGFR to target gene

Depositor

Alexander Marson

Publication

Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CD5 (CD5 Human)

Use

CRISPR

Mutation

WT with tNGFR sequence

Available since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pTR-379 tNGFR-P2A-CD48

Plasmid

#186073

Purpose

Knockin of truncated NGFR to target gene

Depositor

Alexander Marson

Publication

Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

Insert

CD48 (CD48 Human)

Use

CRISPR

Mutation

WT with tNGFR sequence

Available since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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MSCV-mouse Mdm2 wild type-IRES-GFP

Plasmid

#180487

Purpose

Role of Mdm2 variants in tumorigenesis

Depositor

Martine Roussel

Publication

Dang et al Cancer Res. 2002 Feb 15;62(4):1222-30.

Insert

Mouse

Use

Retroviral

Expression

Mammalian

Mutation

wt

Promoter

MSCV

Available since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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C211

Plasmid

#174360

Purpose

For expressing siRNA-resistant human PABPC1 mutant (I110L, D111E, D117E, A121G, G139A, Y140F, T147S, R166K) in human cell lines via transfection

Depositor

David Bartel

Publication

Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

Insert

PABPC1 (PABPC1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

I110L, D111E, D117E, A121G, G139A, Y140F, T147S, …

Available since

Feb. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pDSARN-vasa-eSpCas9sv40

Plasmid

#173670

Purpose

eSpCas9 expression vector for Anopheles

Depositor

Eric Marois

Publication

Klug et al PLoS Pathog. 2022 Oct 12;18(10):e1010881

Insert

eSpCas9 with Anopheles vasa promoter and SV40 terminator

Use

CRISPR

Expression

Insect

Mutation

mutations in Cas9 reducing off-target activity (S…

Promoter

vasa

Available since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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LV_EF1a_PTEN(C124S)-P2A-Hygro_Barcode

Plasmid

#170248

Purpose

Barcoded lentiviral vector to express PTEN (C124S) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

Depositor

Prashant Mali

Publication

Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

Insert

PTEN (PTEN Human)

Use

Lentiviral

Mutation

C124S

Promoter

EF1a

Available since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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EGFP-uTEV1-SQStmd

Plasmid

#171009

Purpose

HiLITR protease with SQS C-terminal targeting information (ER)

Depositor

Alice Ting

Publication

Coukos et al Elife. 2021 Aug 20;10. pii: 69142. doi:

Insert

EGFP-uTEV1-SQS(tmd)

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Promoter

pTRE-tight (TetOn)

Available since

Aug. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pMBP-HRV3C-AcrIF9

Plasmid

#141442

Purpose

Expression of AcrIF9 in E. coli, tagged with an HRV3C-cleavage fusion to Maltose Binding Protein.

Depositor

Blake Wiedenheft

Publication

Hirschi et al Nat Commun. 2020 Jun 1;11(1):2730. doi:

Insert

AcrIF9

Tags

HRV3C cleavage site and Maltose Binding Protein

Expression

Bacterial

Promoter

ptac

Available since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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pCFJ2262A - [1-2] ENTR - Fluor - ce-mNeon (1 intron, syntrons(3), no_atg, no_stop)

Plasmid

#159851

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Publication

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Fluor - ce-mNeon (1 intron, syntrons(3), no_atg, no_stop)

Expression

Worm

Mutation

Not applicable

Available since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pMG035 MBP-Axin∆BCD-His

Plasmid

#154053

Purpose

Expresses MBP-Axin∆BCD-His (human Axin with aa465-518 deletion as a fusion protein with MBP and His- tags) in E.coli

Depositor

Jesse Zalatan

Publication

Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

Insert

Axin1 (AXIN1 Human)

Tags

His6 and MBP

Expression

Bacterial

Promoter

Tac

Available since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pCFJ2245 - [1-2] ENTR - Fluor - ce-tagBFP2 (1 intron, syntrons(3), no_atg, no_stop)

Plasmid

#159843

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Publication

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Fluor - ce-tagBFP2 (1 intron, syntrons(3), no_atg, no_stop)

Expression

Worm

Mutation

Not applicable

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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