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pAGM9121_Sce- α Galactosidase SP

Plasmid

#153504

Purpose

α Galactosidase Signal Peptide Donor Plasmid

Depositor

Sylvestre Marillonnet

Article

Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

Insert

α Galactosidase SP

Use

Synthetic Biology

Available Since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pAGM9121_Aae-UPO* SP

Plasmid

#153506

Purpose

Agrocybe aegerita Unspecific peroxygenase (UPO*) Signal Peptide Donor Plasmid

Depositor

Sylvestre Marillonnet

Article

Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

Insert

Unspecific peroxygenase* SP

Use

Synthetic Biology

Available Since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pAGM9121_Mth-UPO SP

Plasmid

#153507

Purpose

Unspecific peroxygenase Signal Peptide Donor Plasmid

Depositor

Sylvestre Marillonnet

Article

Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

Insert

Unspecific peroxygenase SP

Use

Synthetic Biology

Available Since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pAGM9121_Sce-Invertase 2 SP

Plasmid

#153493

Purpose

S. cerevisiae Invertase Signal Peptide Donor Plasmid

Depositor

Sylvestre Marillonnet

Article

Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

Insert

Invertase 2 SP

Use

Synthetic Biology

Available Since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pAGM9121_Sce-Acid Phosphatase SP

Plasmid

#153494

Purpose

S. cerevisiae Acid Phosphatase Signal Peptide Donor Plasmid

Depositor

Sylvestre Marillonnet

Article

Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

Insert

Acid Phosphatase SP

Use

Synthetic Biology

Available Since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pAGM9121_Gma-UPO SP

Plasmid

#153495

Purpose

Galerina marginata Unspecific Peroxygenase Signal Peptide Donor Plasmid

Depositor

Sylvestre Marillonnet

Article

Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

Insert

Unspecific peroxygenase SP

Use

Synthetic Biology

Available Since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pNAM-B1

Plasmid

#154056

Purpose

Level 0 Golden Gate vector, contains domesticated NAM-B1

Depositor

Cristobal Uauy

Article

Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:

Insert

TaNAM-B1

Use

Synthetic Biology

Expression

Bacterial

Mutation

Synonymous mutation at base 3257, in exon 3, to r…

Promoter

N/A

Available Since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pAGM9121_Mro-UPO SP

Plasmid

#153499

Purpose

Marasmius rotula Unspecific Peroxygenase Signal Peptide Donor Plasmid

Depositor

Sylvestre Marillonnet

Article

Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

Insert

Unspecific peroxygenase SP

Use

Synthetic Biology

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pAGM41991

Plasmid

#153219

Purpose

Level M binary vector for plant transformation; for cloning Cas9 and guide RNAs; single copy in Agrobacterium

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

Type

Empty backbone

Expression

Plant

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pCellFree_G06_ZIKV NS4A

Plasmid

#145356

Purpose

Cell-free expression vector for Zika virus NS4A protein with C-term mCherry

Depositor

Rob Parton

Article

Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

Insert

ZIKV NS4A

Tags

mCherry and 8xHis

Expression

Bacterial

Promoter

T7

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a-Avi-Coh (E154C)-HIS

Plasmid

#153447

Purpose

E. coli expression of Rc. CohE construct with E154C mutation.

Depositor

Michael Nash

Article

Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:

Insert

Rc.CohE

Tags

6xHis and Avi-tag

Expression

Bacterial

Mutation

E154C mutation on CohE

Promoter

T7 promoter

Available Since

July 23, 2020

Availability

Academic Institutions and Nonprofits only

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pFd022.R2

Plasmid

#137967

Purpose

Expresses Prochlorococcus phage P-SSM2 phage Fd

Depositor

Jonathan Silberg

Article

Campbell et al J Biol Chem. 2020 May 19. pii: RA120.013

Insert

Prochlorococcus phage P-SSM2 ferredoxin

Use

Synthetic Biology

Available Since

June 4, 2020

Availability

Academic Institutions and Nonprofits only

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pJTA022

Plasmid

#137975

Purpose

Inucible expression of Prochlorococcus phage P-SSM2 phage Fd for purification

Depositor

Jonathan Silberg

Article

Campbell et al J Biol Chem. 2020 May 19. pii: RA120.013

Insert

Prochlorococcus phage P-SSM2 ferredoxin

Use

Synthetic Biology

Available Since

June 4, 2020

Availability

Academic Institutions and Nonprofits only

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pUC19-hsg(NT:NT)

Plasmid

#138260

Purpose

Expression of a non-targeting sgRNA to the left and right of iCas9 recognition site to be used as a control in Traffic Light reporter system

Depositor

Xiao Wang

Article

Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

Insert

Nontargeting sgRNA

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

March 3, 2020

Availability

Academic Institutions and Nonprofits only

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pSB1C3-FeGFP

Plasmid

#138261

Purpose

Donor-eGFP vector to be used in plasmid to plasmid integration reporter assay. eGFP is conditionally expressed when integrated at iCas9-sites on acceptor plasmid

Depositor

Xiao Wang

Article

Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

Insert

eGFP

Use

CRISPR

Expression

Mammalian

Promoter

None

Available Since

March 3, 2020

Availability

Academic Institutions and Nonprofits only

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pCZGY2750

Plasmid

#135094

Purpose

Site specific CRISPR/Cas9 editing of C. elegans Chr IV

Depositor

Yishi Jin

Article

Andrusiak et al Neuron. 2019 Jul 24. pii: S0896-6273(19)

Insert

sgRNA for cxTi10082 (actgttggatgcctgtgtag)

Use

CRISPR

Expression

Worm

Promoter

U6

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pFB HTB_hMSH6_T1219D

Plasmid

#135466

Purpose

Expression human MSH6_T1219D mutant protein using baculovirus

Depositor

Peggy Hsieh

Article

Geng et al Anal Biochem. 2011 Jun 15;413(2):179-84.

Insert

human MSH6_T1219D (MSH6 Human)

Tags

His-tag

Expression

Insect

Mutation

changed threonine to aspartic acid

Available Since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pSB4C5_J23101-vdh

Plasmid

#128130

Purpose

Expressing vdh gene (for the enzyme transforming benzaldehyde to benzoate) under control of the constitutive promoter J23101, and RBS B0032

Depositor

Jean-Loup Faulon

Article

Pandi et al Nat Commun. 2019 Aug 28;10(1):3880. doi:

Insert

vdh

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23101

Available Since

Nov. 18, 2019

Availability

Academic Institutions and Nonprofits only

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p1147-pCSDest-AcrIIC3Nme-mCherry-miR122BS

Plasmid

#126203

Purpose

Mammalian expression of AcrIIC3Nme fused to mCherry with 3x miR-122 binding sites in 3' UTR

Depositor

Erik Sontheimer

Article

Lee et al RNA. 2019 Aug 22. pii: rna.071704.119. d

Insert

codon-optimized AcrIIC3Nme

Tags

FLAG/NLS and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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p1149-pCSDest-AcrIIA4-mCherry-miR122BS

Plasmid

#126205

Purpose

Mammalian expression of AcrIIA4 fused to mCherry with 3x miR-122 binding sites in 3' UTR

Depositor

Erik Sontheimer

Article

Lee et al RNA. 2019 Aug 22. pii: rna.071704.119. d

Insert

codon-optimized AcrIIA4

Tags

FLAG/NLS and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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