We narrowed to 13,293 results for: Uty

1. LShS-pHG165a E230K

   Plasmid

   #90057

   Purpose

   Constitutive expression of a chimeric LacI:GalS transcription repressor

   Depositor

   Liskin Swint-Kruse

   Insert

   LShS/E230K
   

   Expression

   Bacterial

   Mutation

   E230K

   Promoter

   Iq

   Available Since

   June 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRRlsinPGK_CREGFP_WPRE

   Plasmid

   #91789

   Purpose

   Constitutive expression of creGFP fusion using PGK promoter in a lentiviral backbone.

   Depositor

   Beverly Torok-Storb

   Insert

   Cre-GFP fusion protein
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   PGK

   Available Since

   June 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pENTR11 CA NFAT1

   Plasmid

   #11792

   Depositor

   Anjana Rao

   Insert

   NFAT1 (Nfatc2 Mouse)
   

   Tags

   HA

   Expression

   Bacterial

   Mutation

   Constitutively active: multiple serine to alanine…

   Available Since

   Aug. 7, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJK521

   Plasmid

   #72440

   Purpose

   Produces Acetobacter aceti 1023 small hypothetical protein adjacent to purEK genes (AaOrfX)

   Depositor

   T. J. Kappock

   Insert

   hypothetical protein
   

   Use

   Unspecified

   Mutation

   synthetic gene with ~40 silent substitutions

   Promoter

   n/a

   Available Since

   March 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJK536

   Plasmid

   #72444

   Purpose

   Produces Acetobacter aceti 1023 small hypothetical protein adjacent to purEK genes (AaOrfX)

   Depositor

   T. J. Kappock

   Insert

   hypothetical protein
   

   Expression

   Bacterial

   Mutation

   synthetic gene with ~40 silent substitutions

   Promoter

   T7

   Available Since

   Feb. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCLX-PGK-hCDK4

   Plasmid

   #114314

   Purpose

   Constitutive expression of the CDK4 protein

   Depositor

   Patrick Salmon

   Insert

   CDK4 (CDK4 Human)
   

   Use

   Lentiviral

   Available Since

   Sept. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLNCX chick src Y527F

   Plasmid

   #13660

   Depositor

   Joan Brugge

   Insert

   src Y527F (SRC Chicken)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   Y527F. Constitutive active mutant.

   Available Since

   Jan. 4, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCIG3 Rab5CA

   Plasmid

   #78268

   Purpose

   Rab5 Constitutively active (Q79L) expression

   Depositor

   Felicia Goodrum

   Insert

   RAB5A (RAB5A Human)
   

   Use

   Lentiviral

   Tags

   IRES-EGFP and mCherry

   Expression

   Mammalian

   Mutation

   Q79L

   Promoter

   CMV, Lentiviral LTR

   Available Since

   May 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti-ABE7.10-P2A-Puro

   Plasmid

   #112674

   Purpose

   Lentivirus for constitutive expression of ABE7.10 (not codon optimized)

   Depositor

   Lukas Dow

   Insert

   ABE7.10
   

   Use

   Lentiviral

   Mutation

   D10A

   Promoter

   EF1s

   Available Since

   July 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLNCX chick src E378G

    Plasmid

    #13661

    Depositor

    Joan Brugge

    Insert

    src E378G (SRC Chicken)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    E378G. Constitutive active mutant.

    Available Since

    Jan. 4, 2007

    Availability

    Academic Institutions and Nonprofits only

11. Paxillin_PSCFP2_ mEOS2

    Plasmid

    #114192

    Purpose

    studying focal adhesion proteins by superresolution microscopy (PALM) with dual labelling

    Depositor

    Aleksandra Radenovic

    Insert

    Paxillin (PXN Chicken)
    

    Tags

    PS-CFP2 and mEos2

    Expression

    Mammalian

    Promoter

    SV40

    Available Since

    Oct. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJK523

    Plasmid

    #72442

    Purpose

    Produces Acetobacter aceti 1023 small hypothetical protein adjacent to purEK genes, with N-terminal His6 tag (H6AaOrfX)

    Depositor

    T. J. Kappock

    Insert

    hypothetical protein
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    synthetic gene with ~40 silent substitutions

    Promoter

    T7

    Available Since

    March 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJK553

    Plasmid

    #72452

    Purpose

    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase with N-terminal His6 tag (H6AaPurE1)

    Depositor

    T. J. Kappock

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    synthetic gene with ~40 silent substitutions

    Promoter

    T7

    Available Since

    Feb. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pT2/SV-Venus-SIINFEKL

    Plasmid

    #111624

    Purpose

    Sleeping Beauty transposon containing SV-40-driven Venus-LEQLE-SIINFEKL-TEW for antigen presentation studies

    Depositor

    Jon Yewdell

    Insert

    Venus-LEQLE-SIINFEKL-TEW
    

    Tags

    LEQLE-SIINFEKL-TEW

    Expression

    Mammalian

    Promoter

    SV40

    Available Since

    June 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. hNTo1-qgRNA-pYJA5

    Plasmid

    #217781

    Purpose

    Non-targeting control 1 qgRNA-pYJA5 plasmid from the T.spiezzo library; it can be used as a ready-to-go control and provides the three constant regions for the three-fragment PCRs for qgRNA cloning

    Depositor

    Adriano Aguzzi

    Article

    Yin et al Nat Biomed Eng. 2024 Dec 4. doi: 10.1038

    Insert

    Quadruple sgRNA-expression cassette for nontargeting control for gene ablation
    

    Use

    CRISPR, Lentiviral, and Synthetic Biology

    Expression

    Mammalian

    Promoter

    human U6, mouse U6, human H1, human 7SK

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. hNTo2-qgRNA-pYJA5

    Plasmid

    #217782

    Purpose

    Non-targeting control 2 qgRNA-pYJA5 plasmid from the T.spiezzo library; it can be used as a ready-to-go control and provides the three constant regions for the three-fragment PCRs for qgRNA cloning

    Depositor

    Adriano Aguzzi

    Article

    Yin et al Nat Biomed Eng. 2024 Dec 4. doi: 10.1038

    Insert

    Quadruple sgRNA-expression cassette for nontargeting control for gene ablation
    

    Use

    CRISPR, Lentiviral, and Synthetic Biology

    Expression

    Mammalian

    Promoter

    human U6, mouse U6, human H1, human 7SK

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. Gs(short)-CASE

    Plasmid

    #168124

    Purpose

    Encodes a BRET-based activity sensor for the heterotrimeric G protein Gs. Composed of the subunits G alpha s(short isoform) (GNAS) tagged with NanoLuciferase, G beta 1 (GNB1) and Venus-tagged G gamma 1 (GNG1).

    Depositor

    Gunnar Schulte

    Article

    Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.

    Insert

    GNAS, GNB1, GNG1 (GNGT1, GNAS, GNB1 Human)
    

    Use

    Luciferase

    Tags

    cpVenus on GNG1

    Expression

    Mammalian

    Mutation

    NLuc is inserted at N136/V137 within GNAS

    Available Since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. Go1-CASE

    Plasmid

    #168123

    Purpose

    Encodes a BRET-based activity sensor for the heterotrimeric G protein Go1. Composed of the subunits G alpha o1 (GNAO1) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).

    Depositor

    Gunnar Schulte

    Article

    Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.

    Insert

    GNAO1, GNB3, GNG9 (GNAO1, GNB3, GNGT2 Human)
    

    Use

    Luciferase

    Tags

    cpVenus on GNG9

    Expression

    Mammalian

    Mutation

    NLuc is inserted at L91/G92 within GNAO1

    Available Since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. hNTa2-qgRNA-pYJA5

    Plasmid

    #217780

    Purpose

    Non-targeting control 2 qgRNA-pYJA5 plasmid from the T.gonfio library; it can be used as a ready-to-go control and provides the three constant regions for the three-fragment PCRs for qgRNA cloning

    Depositor

    Adriano Aguzzi

    Article

    Yin et al Nat Biomed Eng. 2024 Dec 4. doi: 10.1038

    Insert

    Quadruple sgRNA-expression cassette for nontargeting control for gene activation and epigenetic silencing
    

    Use

    CRISPR, Lentiviral, and Synthetic Biology

    Expression

    Mammalian

    Promoter

    human U6, mouse U6, human H1, human 7SK

    Available Since

    April 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. hNTa1-qgRNA-pYJA5

    Plasmid

    #217779

    Purpose

    Non-targeting control 1 qgRNA-pYJA5 plasmid from the T.gonfio library; it can be used as a ready-to-go control and provides the three constant regions for the three-fragment PCRs for qgRNA cloning

    Depositor

    Adriano Aguzzi

    Article

    Yin et al Nat Biomed Eng. 2024 Dec 4. doi: 10.1038

    Insert

    Quadruple sgRNA-expression cassette for nontargeting control for gene activation and epigenetic silencing
    

    Use

    CRISPR, Lentiviral, and Synthetic Biology

    Expression

    Mammalian

    Promoter

    human U6, mouse U6, human H1, human 7SK

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge