We narrowed to 14,761 results for: flag
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Plasmid#221329PurposeDox-inducible expression of FLAG-IRSp53-T2A-HA-Cdc42 G12V in mammalian cells by retroviral transductionDepositorUseRetroviralMutationG12VPromoterTRE3GAvailable SinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only
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pLenti-opCas12a-6xNLS
Plasmid#209022PurposeLentiviral vector expressing human codon-optimized OpCas12a with 6xNLSDepositorInsertopCas12a-6xNLS-Flag-Myc-NeoR
UseCRISPR and LentiviralTagsFLAG and MYCExpressionMammalianMutationE174R/S542RPromoterEF-1aAvailable SinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPB CAG SMARCA4-IRES-EGFP
Plasmid#153948PurposepiggyBac transposon vector with CAG promoter expressing wild-type SMARCA4 (FLAG-tagged) and EGFPDepositorAvailable SinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-PHFF-sh-hBVRA
Plasmid#100285PurposeExpression vector for phycocyanobilin (PCB) synthesis and shRNA for human BVRA gene in mammalian cellsDepositorInsertsMTS-PcyA-FLAG-P2A-MTS-HA-HO1-P2A-MTS-Myc-Fd-P2A-MTS-Fnr-T7 (synthetic genes)
shRNA for human BVRA
TagsFLAG, T7 and Mitochondria-targeting sequence (MTS…ExpressionMammalianMutationAll genes are codon-optimized for expression in h…PromoterCAG promoter and H1 promoterAvailable SinceSept. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-F-Med26-NTD R61A K62A
Plasmid#49256PurposeMammalian expression of flag-tagged human Med26-NTD R61A K62ADepositorAvailable SinceFeb. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pU6-(BsaI)_CBh-UC-Cas9-T2A-mCherry
Plasmid#135014PurposeExpression vector for sgRNAs cloned into the BsaI sites and for expression of Cas9 with 126aa MRN-recruiting domain from HSV-1 UL12 fused to C-terminus of Cas9, linked to mCherry via a T2A peptideDepositorInserthumanized S. pyogenes Cas9
Tags3x Flag, 3xFLAG (N terminal on insert), NLS, and …ExpressionMammalianMutation126aa domain from HSV-1 UL12 fused to the C-termi…PromoterCBhAvailable SinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-PHFF-sh-mBVRA
Plasmid#100286PurposeExpression vector for phycocyanobilin (PCB) synthesis and shRNA for mouse BVRA gene in mammalian cellsDepositorInsertsMTS-PcyA-FLAG-P2A-MTS-HA-HO1-P2A-MTS-Myc-Fd-P2A-MTS-Fnr-T7 (synthetic genes)
shRNA for mouse BVRA
TagsFLAG, T7 and Mitochondria-targeting sequence (MTS…ExpressionMammalianMutationAll genes are codon-optimized for expression in h…PromoterCAG promoter and H1 promoterAvailable SinceSept. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pU6-(BsaI)_CBh-UN-Cas9-T2A-mCherry
Plasmid#135013PurposeExpression vector for sgRNAs cloned into the BsaI sites and for expression of Cas9 with 126aa MRN-recruiting domain from HSV-1 UL12 fused to N-terminus of Cas9, linked to mCherry via a T2A peptideDepositorInserthumanized S. pyogenes Cas9
Tags3x Flag, 3xFLAG (N terminal on insert), NLS, and …ExpressionMammalianMutation126aa domain from HSV-1 UL12 fused to the N-termi…PromoterCBhAvailable SinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_Tau Authentic Alu 10-12V337M
Plasmid#194169PurposeInsert: intronic Tau authentic Alu and repeat elements. Introns are flanked by the tau cDNA exons 10-12 with FTLD-Tau V337M mutant. Expresses tau circular RNA 12-->10 VM with 3X flag tag in exon 10.DepositorInsertMicrotubule-associated protein tau 10-12 V337M
Tags3X flagExpressionMammalianMutationChanged Valine 337 to MethinonineAvailable SinceJan. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-CARD14-E138A
Plasmid#69921PurposeExpresses mutant FLAG-CARD14 in mammalian cellsDepositorInsertCARD14 isoform 3 (CARD14 Human)
TagsFLAGExpressionBacterial and MammalianMutationE138APromoterCMVAvailable SinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti-opCas12a-Ultra-6xNLS
Plasmid#209024PurposeLentiviral vector expressing human codon-optimized opCas12a-Ultra with 6xNLSDepositorInsertopCas12a-Ultra-6xNLS-Flag-Myc-NeoR
UseCRISPR and LentiviralTagsFLAG and MYCExpressionMammalianMutationE174R/M537R/S542R/F870LPromoterEF-1aAvailable SinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-FH-AGO2-5xA
Plasmid#91979PurposeExpresses FLAG-HA-AGO2 [S824A, S828A, T830A, S831A, S834A (5xA)]DepositorInsertAGO2 (AGO2 Human)
UseLentiviralTagsFLAG-HAExpressionMammalianMutationS824A, S828A, T830A, S831A, S834A (5xA)Available SinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Cterm_3xFL_BRD9_BET-PGK-Puro-IRES-GFP
Plasmid#75123PurposeRetroviral expression plasmid encoding a human BRD9 bromodomain-swap mutant containing the first bromodomain of BRD4 with a C terminal 3x FLAG-tagDepositorInsertBRD9 (BRD9 Human)
UseRetroviralTags3x FLAG-tagMutationbromodomain replaced with the first bromodomain o…Available SinceMay 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-FH-AGO2-5xE
Plasmid#91981PurposeExpresses FLAG-HA-AGO2 [S824E, S828E, T830E, S831E, S834E (5xE)]DepositorInsertAGO2 (AGO2 Human)
UseLentiviralTagsFLAG-HAExpressionMammalianMutationS824E, S828E, T830E, S831E, S834E (5xE)Available SinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Puro-FH-AGO2-5xE
Plasmid#91993PurposeExpresses FLAG-HA-AGO2 [S824E, S828E, T830E, S831E, S834E (5xE)]DepositorInsertAGO2 (AGO2 Human)
UseRetroviralTagsFLAG-HAExpressionMammalianMutationS824E, S828E, T830E, S831E, S834E (5xE)Available SinceJan. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Puro-FH-AGO2-5xA
Plasmid#91992PurposeExpresses FLAG-HA-AGO2 [S824A, S828A, T830A, S831A, S834A (5xA)]DepositorInsertAGO2 (AGO2 Human)
UseRetroviralTagsFLAG-HAExpressionMammalianMutationS824A, S828A, T830A, S831A, S834A (5xA)Available SinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-CARD14-G117S
Plasmid#69920PurposeExpresses mutant FLAG-CARD14 in mammalian cellsDepositorInsertCARD14 isoform 3 (CARD14 Human)
TagsFLAGExpressionBacterial and MammalianMutationG117SPromoterCMVAvailable SinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPB CAG SMARCA4 T910M-IRES-EGFP
Plasmid#153949PurposepiggyBac transposon vector with CAG promoter expressing T910M mutant SMARCA4 (FLAG-tagged) and EGFPDepositorAvailable SinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-CARD14-D176H
Plasmid#69919PurposeExpresses mutant FLAG-CARD14 in mammalian cellsDepositorInsertCARD14 isoform 3 (CARD14 Human)
TagsFLAGExpressionBacterial and MammalianMutationD176HPromoterCMVAvailable SinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET11d-FHis-DND1-Y102A
Plasmid#70082PurposeBacterial expression of FLAG-and 6xHis-tagged human DND1 protein with defective RRM (Y102A). Codons are optimized for bacteria expression.DepositorInsertDnd1 (DND1 Human)
Tags6xHIS and FLAGExpressionBacterialMutationY102A. Codon optimized for bacterial expressionAvailable SinceNov. 22, 2017AvailabilityAcademic Institutions and Nonprofits only