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pcDNA3.1-TK1

Plasmid

#100544

Purpose

mammalian expression of thymidine kinase 1

Depositor

Yuh-Shan Jou

Article

Yeh et al Oncotarget. 2017 Sep 8;8(44):77734-77751

Insert

thymidine kinase 1 (TK1 Human)

Expression

Mammalian

Available Since

Sept. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP(C3)-Nup153-N

Plasmid

#64318

Purpose

GFP-tagged mammalian expression of N-terminal domain of Nup153

Depositor

Birthe Fahrenkrog

Article

Duheron et al Nucleus. 2014;5(6):601-12. doi: 10.4161/

Insert

Nup153 (NUP153 Human)

Tags

GFP

Expression

Mammalian

Mutation

N-terminal domain

Promoter

CMV

Available Since

April 23, 2015

Availability

Academic Institutions and Nonprofits only
pAAV-nEF Con/Foff hChR2(H134R)-EYFP

Plasmid

#55647

Purpose

Cre-on/Flp-off ChR2-EYFP under the short Ef1a promoter

Depositor

Karl Deisseroth

Article

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Insert

ChR2(H134R)-EYFP

Use

AAV; Cre on/flp off chr2-eyfp

Tags

EYFP

Expression

Mammalian

Promoter

Short Ef1a

Available Since

Aug. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pLKO.1-sh-mSOX9-2

Plasmid

#40645

Depositor

Bob Weinberg

Article

Guo et al Cell. 2012 Mar 2;148(5):1015-28.

Insert

sh-mSOX9-2 (Sox9 Mouse)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

hU6

Available Since

Oct. 29, 2012

Availability

Academic Institutions and Nonprofits only
pQexp

Plasmid

#25820

Depositor

George Church

Article

Tolonen et al Mol Microbiol. 2009 Dec . 74(6):1300-13.

Insert

pQexp

Expression

Bacterial

Available Since

Sept. 14, 2010

Availability

Academic Institutions and Nonprofits only

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Rogi1-CMV-LAMB3-T2A-GFP-bGH

Plasmid

#179864

Purpose

Targets Rogi1 genomic region with HDR and expresses LAMB3-T2A-GFP

Depositor

George Church

Article

Aznauryan et al Cell Rep Methods. 2022 Jan 14;2(1):10015

Insert

LAMB3-T2A-GFP

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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ITPKA-mScarlet-I

Plasmid

#98829

Purpose

In vivo visualization of actin cytoskeleton (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

ITPKA

Tags

mScarlet-I

Expression

Mammalian

Promoter

CMV

Available Since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-sCre

Plasmid

#55635

Purpose

Expresses sCre in Mammalian Cells

Depositor

Karl Deisseroth

Article

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Insert

Cre

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

EF1alpha

Available Since

July 10, 2014

Availability

Academic Institutions and Nonprofits only

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pQCXIP X2 DEST (w270-1)

Plasmid

#17474

Purpose

Retroviral Gateway destination vector, RNAi, 3’LTR insertion, Puro

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Gateway Cloning, RNAi, and Retroviral

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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p(HA)HIF1alpha(401delta603)R27G

Plasmid

#52215

Purpose

mammalian expression of HA tagged HIF1alpha with a deletion of the N-terminal activation domain and R27G mutation

Depositor

Eric Huang

Article

Koshiji et al EMBO J. 2004 May 5;23(9):1949-56. Epub 2

Insert

HIF1alpha (401delta603) R27G (HIF1A Human)

Tags

HA

Expression

Mammalian

Mutation

aa 401-603 deleted, removes the oxygen-dependent …

Promoter

CMV

Available Since

April 7, 2014

Availability

Academic Institutions and Nonprofits only
pCAG-FRT-stop-FRT(FSF)- loxP-stop-loxP(LSL)-SP-HA-HRPtm-WPRE-bGHpolyA

Plasmid

#197044

Purpose

In situ cell-surface proteome extraction by extracellular labeling (iPEEL)

Depositor

Liqun Luo

Article

Shuster et al Neuron. 2022 Dec 7;110(23):3882-3896.e9.

Inserts

pCAG-FSF-LSL
SP-HA-HRPtm
WPRE-bGHpolyA

Tags

HA, myc

Expression

Mammalian

Promoter

pCAG

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-ClpX

Plasmid

#234733

Purpose

Human-codon optimized E. coli ClpX in mammalian cells

Depositor

David Liu

Article

Witte et al Science. 2025 May 15;388(6748):eadt5199.

Insert

ClpX

Expression

Mammalian

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2+ H2B-mCherry

Plasmid

#99265

Purpose

Contains a fusion of human histone H2B to fluorescent protein mCherry in the pCS2+ expression vector. It has the SP6 RNA polymerase site for in vitro transcription.

Depositor

Philipp Keller

Article

Wan et al Cell. 2019 Oct 3;179(2):355-372.e23. doi

Insert

mCherry

Tags

H2B

Expression

Mammalian

Promoter

SP6

Available Since

Sept. 6, 2017

Availability

Academic Institutions and Nonprofits only

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FUGW-Mito-TurboID

Plasmid

#200963

Purpose

3rd gen lentiviral plasmid for expressing mitochondria localized TurboID with EGFP

Depositor

Keriann Backus

Article

Yan et al Cell Chem Biol. 2023 Jul 20;30(7):811-82

Insert

Mito-TurboID

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Available Since

Aug. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pSAM2_mCherry_Gata4

Plasmid

#72690

Purpose

Doxycyline regulated expression of Gata4 in mammalian cells

Depositor

Timothy Kamp

Article

Lalit et al Cell Stem Cell. 2016 Mar 3;18(3):354-67.

Insert

Gata 4 (Gata4 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV-Doxycycline

Available Since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pGFP-ATIC

Plasmid

#99110

Purpose

Expresses human GFP-ATIC fusion in mammalian cells

Depositor

Stephen Benkovic

Article

An et al Science. 2008 Apr 4;320(5872):103-6. doi

Insert

ATIC (ATIC Human)

Tags

GFP

Expression

Mammalian

Mutation

S65T mutation in GFP gene

Promoter

CMV

Available Since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pTetON-DeAct-SpvB

Plasmid

#89455

Purpose

Tet/Dox-inducible expression of EGFP-SpvB (Salmonella SpvB 375-591) in mammalian cells

Depositor

Brad Zuchero

Article

Harterink et al Nat Methods. 2017 Apr 10. doi: 10.1038/n

Insert

DeAct-SpvB (spvB Salmonella enterica)

Tags

EGFP

Expression

Mammalian

Mutation

Truncation spanning SpvB amino acids 375-591 (mon…

Promoter

TRE3G

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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Lenti.pFOXA2.GFP

Plasmid

#59404

Purpose

expresses eGFP from a 400 bp human FOXA2 promoter to select floor plate and ventral midbrain progenitor cells during development

Depositor

Kai-Christian Sonntag

Article

Aguila et al Stem Cells Transl Med. 2014 Jul 14. pii:

Insert

400 bp FOXA2 promoter, eGFP (FOXA2 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

pFOXA2

Available Since

April 19, 2016

Availability

Academic Institutions and Nonprofits only

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CMVp-dCas9-3xNLS-VP64 (Construct 1)

Plasmid

#55195

Purpose

Expresses taCas9 in Mammalian cells for transactivating endogenous and synthetic promoters. The backbone is a lentiviral vector.

Depositor

Timothy Lu

Article

Nissim et al Mol Cell. 2014 May 14. pii: S1097-2765(1

Insert

dCas9

Use

CRISPR and Synthetic Biology

Tags

VP64

Expression

Mammalian

Promoter

CMV/hUBC

Available Since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR - EGFP sgRNA 5

Plasmid

#51764

Purpose

Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

Depositor

Feng Zhang

Article

Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi:

Inserts

Cas9
Puromycin resistance
EGFP sgRNA 5

Use

CRISPR and Lentiviral

Promoter

EFS and U6

Available Since

March 11, 2014

Availability

Academic Institutions and Nonprofits only

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